| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 613,399 | T→C | 6.1% | G44G (GGT→GGC) | U712_03020 → | 60 kDa chaperonin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 613,399 | 0 | T | C | 6.1% | 199.9 / 4.2 | 65 | G44G (GGT→GGC) | U712_03020 | 60 kDa chaperonin |
| Reads supporting (aligned to +/- strand): ref base T (38/23); new base C (2/2); total (40/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
CATGGCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACGGTACAACAACT > CP006881/613267‑613537 | cATGGCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCg > 7:2109/1‑139 (MQ=255) aTGGCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGt > 1:267732/1‑139 (MQ=255) aTGGCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATACATACAGATGTCGAATCACATTCTGATGATTTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTATATAGAAAAAATTAGCTTCACCgg > 2:172131/1‑138 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGtt > 4:143638/1‑131 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGtt < 3:143638/131‑1 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCg < 8:217378/136‑1 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCg > 7:217378/1‑136 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGtt > 5:311743/1‑138 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGtt > 6:150262/1‑138 (MQ=255) ggCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTa > 1:315611/1‑139 (MQ=255) aaGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATcac < 7:46934/139‑1 (MQ=255) ttAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGa < 8:101828/139‑1 (MQ=255) ttAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGa < 6:88255/139‑1 (MQ=255) ttAAGTTTAGTGAAGAAGCGCGCCGCGCAATGCTTCGCGGTTGCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGa < 6:235795/139‑1 (MQ=255) tatgTTTCGTTAAGACGGCCGCCGCGAATTGATTGGCGGTGCGTATGCACTTGCTGATGCTGTTAATTAAACTTATGGACAAAAAGGACGCAACGTGTTTCTAGACAAAAAATTCGGTTCTCCGTTAATCACAAATGa < 2:173908/135‑1 (MQ=255) tGAAGAAGCTCGCCGCGAAATGCTTCGCGGTGTCGATGAATTTGCTGATGCTGTTAATGTAACTTTAGGACCAAATGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAAc < 6:311743/138‑1 (MQ=255) cgccgcGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTa > 3:163998/1‑136 (MQ=255) cgcgGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTACTCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTagaag > 4:198207/1‑139 (MQ=255) ggTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGAAGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGAcg > 1:162282/1‑139 (MQ=255) tgtCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGa < 8:300367/99‑1 (MQ=255) tgtCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGa > 7:300367/1‑99 (MQ=255) tgtCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACTGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGAcgcg > 7:289748/1‑139 (MQ=255) tCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGtt > 8:211241/1‑139 (MQ=255) tGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGaa < 3:273924/139‑1 (MQ=255) cTTGCTGATGCTGTTAAAATAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCTAGCTATACGACGCGTTTGAAGACa > 2:107319/1‑139 (MQ=255) tGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACa > 7:179643/1‑137 (MQ=255) tGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACa < 8:179643/137‑1 (MQ=255) tGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATg > 6:281896/1‑139 (MQ=255) tGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATg > 6:21243/1‑139 (MQ=255) tGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATg > 8:332534/1‑139 (MQ=255) gCTGATGCTGTTAAAGTATCTTTAGGACCAAAATGACGCAACGTTGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt < 8:73474/137‑1 (MQ=255) tGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAg > 8:66398/1‑139 (MQ=255) tGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAg > 4:85352/1‑139 (MQ=255) ttAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCTAGCTAGCAGACGCGGTTGAAAACATGGGTGCTAAGCt > 5:290518/1‑139 (MQ=255) ttAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt > 4:182678/1‑127 (MQ=255) ttAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt < 3:182678/127‑1 (MQ=255) aCTTTAGGACCAAAAGGACGCACCGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCTAGCTAGAAGCCGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCt > 6:9646/1‑138 (MQ=255) aCTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCt > 1:160654/1‑138 (MQ=255) aCTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCt > 7:189782/1‑138 (MQ=255) aCTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCt > 5:191268/1‑138 (MQ=255) aCTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCACCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTCTTGCt > 5:132252/1‑138 (MQ=255) tttAGGACCTAAAGTACGCAACGTGGTTATAGAGAAAATATTCGGTTCTACGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGaa < 6:290518/139‑1 (MQ=255) tttAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCg < 1:7717/80‑1 (MQ=255) tttAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCg > 2:7717/1‑80 (MQ=255) tttAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACaa < 5:257023/117‑2 (MQ=255) tttAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt > 6:257023/1‑117 (MQ=255) ggACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTa < 5:150262/138‑1 (MQ=255) gACCAACAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGc < 4:230357/139‑1 (MQ=255) gACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGc < 7:211241/139‑1 (MQ=255) aaaGGACGCAACGTGGTTCTATAGAAAAAATTCGGTTCTCCGGTATTCACAAATGACGGTGTAACAAACGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAATCATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGc > 5:335135/1‑138 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt > 1:333477/1‑107 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt < 2:333477/107‑1 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATggg > 5:212901/1‑110 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATggg < 6:212901/110‑1 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGc > 7:183137/1‑138 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGc > 7:57776/1‑138 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGc > 1:310392/1‑138 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGCTCTCCGTTAATCACAAATGACGGTGTAACa > 3:335353/1‑66 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGCTCTCCGTTAATCACAAATGACGGTGTAACa > 3:330176/1‑66 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGCTCTCCGTTAATCACAAATGACGGTGTAACa < 4:330176/66‑1 (MQ=255) aaaGGACGCAACGTGGTTCTAGAGAAAAAATTCGGCTCTCCGTTAATCACAAATGACGGTGTAACa < 4:335353/66‑1 (MQ=255) cGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAaaaca > 1:52743/1‑138 (MQ=255) aaaaaaTCCGGTTCTCCGTTAATCACAAATGACGGTGTAACAAACGCTAAAGAAATCGAGCAAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACg < 7:148770/139‑1 (MQ=255) aaaaTTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt < 3:214083/81‑1 (MQ=255) aaaaTTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACAt > 4:214083/1‑81 (MQ=255) aaaaTTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACgg > 5:212721/1‑138 (MQ=255) gTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTTAAAACATGGGGGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACGGTACaacaac < 5:281896/139‑1 (MQ=255) ttCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACGGTACAACAACt < 7:66398/139‑1 (MQ=255) | CATGGCAAAAGAAATTAAGTTTAGTGAAGAAGCTCGCCGCGCAATGCTTCGCGGTGTCGATGCACTTGCTGATGCTGTTAAAGTAACTTTAGGACCAAAAGGACGCAACGTGGTTCTAGAGAAAAAATTCGGTTCTCCGTTAATCACAAATGACGGTGTAACAATCGCTAAAGAAATCGAGCTAGAAGACGCGTTTGAAAACATGGGTGCTAAGCTTGTTGCTGAAGTAGCCAGCAAAACAAACGACGTTGCCGGTGACGGTACAACAACT > CP006881/613267‑613537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |