BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,006	T→G	5.9%	V40G (GTT→GGT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,006	0	T	G	5.9%	5053.1 / 45.4	2027	V40G (GTT→GGT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (893/958); new base G (149/17); total (1050/977)
Fisher's exact test for biased strand distribution p-value = 1.24e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2038 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

     acacaGTAAACAGCTATGACCATGATTACGCAAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAGTTCACTGGCCTTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  8:15526/138‑1 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAccc                                                                                                                                       <  1:254918/139‑1 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCTGGACTCGGAAAACCCTGGCGTTACCCAAc                                                                                                                                    >  8:253785/1‑138 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTTACCCCACt                                                                                                                                   >  7:7213/1‑139 (MQ=255)

            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  4:37084/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                             <  4:214921/139‑1 (MQ=255)

                   tATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGccgt                                                                                                                         >  4:309366/1‑137 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                               >  3:285621/1‑131 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                     <  2:270842/139‑1 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCGGGCGGTGCCCAACTTAATCGCCTTgcag                                                                                                                     >  8:277989/1‑139 (MQ=255)

                          aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                  >  6:67947/1‑139 (MQ=255)

                           tGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGCAAACCCGGGCGGTACCCAACGTAATAGCCGTgcagcctg                                                                                                                 >  2:41223/1‑136 (MQ=255)

                           tGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                       <  2:96846/133‑1 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCGGGCGTTACCCAACGTAATCGCCGTGCAGcacct                                                                                                                >  3:119362/1‑137 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  1:296263/115‑1 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcagcccct                                                                                                                >  6:355404/1‑135 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  8:190170/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  8:172995/1‑139 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGGCTGGGTAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGcacct                                                                                                                >  6:98500/1‑137 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGCAACCCCTGGCGTTACCCCACGTAATCGCCTTGCAGcccag                                                                                                                >  2:64370/1‑138 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTAAAACCTCGGTCCGGGGGAAACCCCGGCGGTGCCCCCCATAATAGCCCTGGAGcacca                                                                                                                >  2:159259/1‑137 (MQ=255)

                             aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                               <  2:238998/139‑1 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  3:45516/113‑1 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                      >  3:148425/1‑139 (MQ=255)

                                           gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  5:18747/139‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                        <  4:55537/115‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  4:283409/138‑1 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 >  2:10595/1‑132 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCCGGGCCGTACCCCACTTAATAGCCGTTCAGCACATCCCCCATTCGCCACCAGGCGt                                                                                           >  4:254745/1‑138 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTAAAACGTCGGGGCGGGGGAAACCCGGGCGGTTACCAACTAAATACCCTTTGCGCCCAACCCCCATTAGGCAGCgggggg                                                                                           >  5:14357/1‑135 (MQ=255)

                                                   gCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                         <  5:359901/139‑1 (MQ=255)

                                                    cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                         <  3:231925/138‑1 (MQ=255)

                                                         agagtctAGAGGA‑GCCCGGGTACGGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGac                                                                                  <  8:299732/135‑2 (MQ=255)

                                                          gACTCTCGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCCaa                                                                                  <  6:47636/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCCGCTGGGGGATTAGCg                                                                                    >  7:98272/1‑137 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCGTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  6:319134/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  6:178772/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  3:256055/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  5:272750/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  4:73532/139‑1 (MQ=255)

                                                          gACTCTAGAGGAGCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGGGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGaa                                                                                  <  5:305821/139‑1 (MQ=255)

                                                          gACGCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGTGAAAACCCGGGGGTTACCCAACTTAATCGCCTTGCAGAACATCCCCCTTTCGCCAGCTGGCGTATTAGCGaa                                                                                  <  1:75473/139‑1 (MQ=255)

                                                          acagctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  1:362027/135‑1 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCAACCCCCTTTCTCCAGCTGGCGTAATAGcagtc                                                                                 >  8:364289/1‑135 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAag                                                                                 <  5:112101/139‑1 (MQ=255)

                                                            ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  5:334279/122‑1 (MQ=255)

                                                                 gagGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTGGTTTTTCAACGTCGTGACTGGGAAAACCCTGGCGTTTACCAACATAATAGCCATGCAGCCCATCCCCCTTTCGCCATCTGGGGGAATAGCGAATAGGgcc                                                                           >  4:161140/1‑139 (MQ=255)

                                                                     aTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCATTCGCCCCCGGGCCTAATAGCGAAGAGGCCCGCAc                                                                       >  1:51852/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGGCTGGGGAAACCCGGGCGGTTCCCCACATAATAGCCTTGCCGCCCAAACCCCGTTCCCCAGCGTGCGGAATTACGCAGAGGCCCGcccc                                                                      >  3:6106/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTAAACGTAGTGACTGGGAAAACCCAGGCGGTTCCCAAAATAATAGCCATTACGCCCCAACCCCTTTATCCACCGGGCGGAATAGACAATAGGCCCCCAcc                                                                      >  4:335/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                      >  7:13601/1‑107 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGGAATAGCGAAGAGGCCCGcccc                                                                      >  1:220637/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGcccc                                                                      >  3:309865/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGcccc                                                                      >  7:276596/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  1:2052/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  2:214820/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATAGCCTTGCCGCCCATCCCCCCTTCGCCACCTGGCGTAATATCGAAGAGGcccccccc                                                                      >  3:86191/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCCTGGCGTTTCCCAACATAAAACCCTTGCCGCCCAGCCCCCATTCGCCCCCTGGCGGTATATAGAAGAGGcccccccc                                                                      >  1:128896/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGCGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  3:356889/127‑1 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                               >  6:297766/1‑81 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                               <  8:348466/97‑1 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                            <  8:122691/116‑1 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCg                                                                     >  3:176024/1‑139 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTGGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  7:196527/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  8:208839/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  7:353837/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACTACGTCGTGACTGGGAAAAGCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:48660/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:325932/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:303776/139‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  8:57855/139‑1 (MQ=255)

                                                                             gTACCGAGCGCGAATTCACTGGCCGTCGTGTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCTGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  2:338890/139‑1 (MQ=255)

                                                                              tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTTCCCAACTTAATCGCCATGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATTGCGAAGAGGCCCCCCCCCATAGccc                                                              >  7:24146/1‑139 (MQ=255)

                                                                              tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                              <  7:1549/139‑1 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        >  8:227635/1‑63 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCtt                                                            <  4:23192/139‑1 (MQ=255)

                                                                                 cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATATCGAAGATGCCCGCCCCGATCGCCCtga                                                           >  6:140658/1‑137 (MQ=255)

                                                                                  gAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTcc                                                          >  6:163123/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACGTAATCGCCTTGCAGCCCATCCCCCATTCGCCACCGGGCGGAATAGCGAAGAGGCCCGCCCCGCTCGCCCATccc                                                         >  4:140566/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  1:183617/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  6:209119/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  1:1464/139‑1 (MQ=255)

                                                                                     cTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCCCCGATCGCCCTTCCCaa                                                       >  3:331696/1‑139 (MQ=255)

                                                                                        gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGGAGCACAAGCCCCGTTAGCCAGCTGGCGGTATATCGAAGAGGCCCCCACCGCTCCCCCGTCCCAAccg                                                    >  3:193679/1‑137 (MQ=255)

                                                                                        gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCCCCTCCCCCTTTCGCCCGCTGGCGTAAATGCGAAGAGGCCCGCCCCCAACGCCCTTCCCAACAg                                                    >  2:203784/1‑139 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGGGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  8:75604/139‑1 (MQ=255)

                                                                                                              cAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                              <  6:119021/139‑1 (MQ=255)

                                                                                                                 cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                           >  5:91793/1‑139 (MQ=255)

                                                                                                                 cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCCCCCCGATCGCCCTTCCCGCCAGTTGCGCAGCCTgaatggcgaatggc                           >  5:164364/1‑139 (MQ=255)

                                                                                                                   tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                         >  4:113523/1‑139 (MQ=255)

                                                                                                                   tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                         <  6:333577/139‑1 (MQ=255)

                                                                                                                    cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                        <  7:172995/139‑1 (MQ=255)

                                                                                                                           gggAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  5:71179/139‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  7:22234/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  4:184273/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  1:10249/138‑1 (MQ=255)

                                                                                                                                  cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          >  6:249132/1‑139 (MQ=255)

                                                                                                                                  cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGAGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          <  2:301733/139‑1 (MQ=255)

                                                                                                                                  cccTGGCGTTACCCAACTTAATCGCCTGGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          <  1:334250/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        >  8:323878/1‑139 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  5:248963/48‑1 (MQ=255)

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`