Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 727,843 A→G 5.1% K10R (AAG→AGG)  U712_03535 → Unsaturated rhamnogalacturonyl hydrolase yesR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881727,8430AG 5.1% 255.9 / 3.7 82K10R (AAG→AGG) U712_03535Unsaturated rhamnogalacturonyl hydrolase yesR
Reads supporting (aligned to +/- strand):  ref base A (44/32);  new base G (2/2);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

GTCAGCCGTCTCGTTATTGCCTGTGATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATCTGT  >  CP006881/727709‑727980
                                                                                                                                      |                                                                                                                                         
gTCAGCCGTCTCGTTATTGCCTGTGATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGt                                                                                                                                        >  1:225886/1‑138 (MQ=255)
    gCCGTCTCGTTATTGCCTGTGATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGt                                                                                                                                   <  5:282636/139‑1 (MQ=255)
                     tgtgGTTCTTGTTTTGTTTTTGTTTCAGAAATATATCGTTAAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTTTTGCAGATGGCACAGGTTATCTTTGATGAAGAAAAGGTGACAACGGTTTTTGACCGGATc                                                                                                                  >  4:218128/1‑139 (MQ=255)
                      gtgATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCg                                                                                                                 >  8:310224/1‑139 (MQ=255)
                      gtgATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCg                                                                                                                 >  8:47480/1‑139 (MQ=255)
                       tgATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGt                                                                                                                >  6:124476/1‑139 (MQ=255)
                       tgATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGt                                                                                                                >  1:363935/1‑139 (MQ=255)
                             ttGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACg                                                                                                          <  2:5932/139‑1 (MQ=255)
                                ttttCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGa                                                                                                        <  3:100175/138‑1 (MQ=255)
                                     tttttGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACAttt                                                                                                   >  4:242385/1‑138 (MQ=255)
                                         tGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               <  2:225886/138‑1 (MQ=255)
                                             tCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTAGGTTATTGACCGGATCGTGAAACGGACATTTCAGAAGGAt                                                                                          >  7:205798/1‑139 (MQ=255)
                                               aGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAATATAAAAAGGAGTGTTGCAGATGGCCCAGCTTATCTTTGATGAAGAAAAGGAGAAGTCCGTTAGTGACCGGATCGTGTCTCGGACATTTAAGATGGAgtt                                                                                        >  8:170304/1‑139 (MQ=255)
                                                      tatCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTgg                                                                                 >  7:32561/1‑139 (MQ=255)
                                                           ttCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGCCCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTg                                                                            >  1:204492/1‑139 (MQ=255)
                                                           ttCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTg                                                                            >  1:351152/1‑139 (MQ=255)
                                                           ttCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTg                                                                            >  5:250960/1‑139 (MQ=255)
                                                           ttCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTg                                                                            >  4:56575/1‑139 (MQ=255)
                                                           ttCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTg                                                                            >  3:133765/1‑139 (MQ=255)
                                                                ggCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCgg                                                                       >  3:122785/1‑139 (MQ=255)
                                                                 gCATCAGTACGACCGGATTATAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGACGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCggg                                                                      <  6:308504/139‑1 (MQ=255)
                                                                 gCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCggg                                                                      <  2:234237/139‑1 (MQ=255)
                                                                 gCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCggg                                                                      <  3:309886/139‑1 (MQ=255)
                                                                     cAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGggcggc                                                                  >  6:245598/1‑139 (MQ=255)
                                                                     cAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGggcggc                                                                  >  7:167433/1‑139 (MQ=255)
                                                                        tACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               <  2:32317/139‑1 (MQ=255)
                                                                        tACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATAGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgagtt                                                               <  8:210008/139‑1 (MQ=255)
                                                                         aCGACCGTATTAACATAAAAAGGAGTGTTGTAGATGGCACTGCTTATCTTTGATGATGTAATTGTGAAGTCGGTTATTGATATGATCGTGAAACGGACATTTCAGTTGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               <  2:363935/138‑1 (MQ=255)
                                                                         aCGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               <  8:167433/138‑1 (MQ=255)
                                                                          cGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgttgc                                                             >  2:225477/1‑139 (MQ=255)
                                                                            aCCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGGTATTGACCGGAACGTGAAACGGACATTTCAGATGGATTTTGCGTTGGATTGGCCGGGCGgcggtgcgg                                                           >  2:317469/1‑138 (MQ=255)
                                                                              cgtaTTAAAATAAAACGGTGGGGTGCTGATGGAACTGCTTATCTTTGTTGAATTAAAGGTGACGTCGGGTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGttt                                                         <  1:317469/136‑1 (MQ=255)
                                                                              cGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGttt                                                         <  3:242385/139‑1 (MQ=255)
                                                                                gATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       <  1:225477/139‑1 (MQ=255)
                                                                                gATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       <  5:245598/139‑1 (MQ=255)
                                                                                  ttAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAGGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               >  7:44777/1‑97 (MQ=255)
                                                                                  ttAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAGGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               <  8:39423/97‑1 (MQ=255)
                                                                                  ttAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAGGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               >  7:39423/1‑97 (MQ=255)
                                                                                  ttAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAGGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               <  8:44777/97‑1 (MQ=255)
                                                                                          aaaaGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGATATGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGGTGGGTTTTACGGTGTgggg                                             >  3:187489/1‑137 (MQ=255)
                                                                                           aaaGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGt                                                                                                                                   >  8:306734/1‑52 (MQ=255)
                                                                                           aaaGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGt                                                                                                                                   <  7:306734/52‑1 (MQ=255)
                                                                                           aaaGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTTGGGTTTTCGGGGTGgggg                                            >  2:184294/1‑139 (MQ=255)
                                                                                            aaGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGa                                           <  2:204492/139‑1 (MQ=255)
                                                                                                  tgtTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       >  3:362100/1‑121 (MQ=255)
                                                                                                  tgtTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       <  4:362100/121‑1 (MQ=255)
                                                                                                  tgtTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGa                                           <  6:92323/133‑1 (MQ=255)
                                                                                                  tgtTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGa                                           >  5:92323/1‑133 (MQ=255)
                                                                                                  tgtTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTa                                     >  3:16346/1‑139 (MQ=255)
                                                                                                         gATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               >  4:308356/1‑74 (MQ=255)
                                                                                                         gATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGa                                                                                               <  3:308356/74‑1 (MQ=255)
                                                                                                             gacaggCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACgg                          <  1:171559/134‑1 (MQ=255)
                                                                                                             gCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACg                           >  5:245436/1‑138 (MQ=255)
                                                                                                              cacaGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTTTGGGGGATGCCTATGAAGAGACgg                          >  6:49059/1‑138 (MQ=255)
                                                                                                              cacaGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCg                                             <  1:292908/119‑1 (MQ=255)
                                                                                                              cacaGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCg                                             >  2:292908/1‑119 (MQ=255)
                                                                                                                 aGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGaaaa                      >  3:167050/1‑139 (MQ=255)
                                                                                                                      aTCTTTGATGAAGAAAAGGGGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAg                  <  1:184294/138‑1 (MQ=255)
                                                                                                                           tgatgaAGAAAAGGTGCCGTCGGTTATTGACCGGATCGTGAATCGGACATTTCAGATGGATTTTGAGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAatat            >  6:288823/1‑139 (MQ=255)
                                                                                                                           tgatgaAGAAAAGGTGACGGCGGTTATTGACCGGATCGTGAACCGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGa                <  3:208325/135‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAg                                                                                                <  4:8359/53‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAg                                                                                                >  3:8359/1‑53 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               <  3:22721/86‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               >  8:24183/1‑86 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               <  7:24183/86‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGgcgtt                                                               >  4:22721/1‑86 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       <  7:243615/94‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTa                                                       >  8:243615/1‑94 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGa                                           >  7:90118/1‑106 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGa                                           <  8:90118/106‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGa                <  4:87689/133‑1 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGa                >  3:87689/1‑133 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAatatat          >  4:332645/1‑139 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAatatat          >  8:267220/1‑139 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAatatat          >  3:355568/1‑139 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAatata           >  7:79427/1‑138 (MQ=255)
                                                                                                                             atgaAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGAAGGAAAACGAGGAatata           >  5:94420/1‑138 (MQ=255)
                                                                                                                                  gaaAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTa                                     >  6:66979/1‑107 (MQ=255)
                                                                                                                                  gaaAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTa                                     <  5:66979/107‑1 (MQ=255)
                                                                                                                                  gaaAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATc     <  4:122785/139‑1 (MQ=255)
                                                                                                                                  gaaAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATc     >  1:256692/1‑139 (MQ=255)
                                                                                                                                  gaaAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTGGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATc     <  7:310224/139‑1 (MQ=255)
                                                                                                                                     aaGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATc     >  4:210876/1‑136 (MQ=255)
                                                                                                                                     aaGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATc     <  3:210876/136‑1 (MQ=255)
                                                                                                                                     aaGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATCTGt  >  7:32121/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                         
GTCAGCCGTCTCGTTATTGCCTGTGATTCTTGTTTTCTTTTTGTTTCAGAAATATATCGTTCAGGGCATCAGTACGACCGGATTAAAATAAAAAGGAGTGTTGCAGATGGCACAGCTTATCTTTGATGAAGAAAAGGTGACGTCGGTTATTGACCGGATCGTGAAACGGACATTTCAGATGGATTTTGCGTGGGATTGGCCGGGCGGCGTTGCGTTTTACGGTGTGGCGGAGGCCTATGAAGCGACGGAAAACGAGGAATATATCAATCTGT  >  CP006881/727709‑727980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑