Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 995,011 A→G 8.2% K195E (AAA→GAA)  U712_04855 → 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881995,0110AG 8.2% 146.0 / 4.5 49K195E (AAA→GAA) U712_048551‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (15/30);  new base G (2/2);  total (17/32)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GAATTATTCAAAAAAGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAGAAGTTCTTCTTCTTGAAGCTTT  >  CP006881/994873‑995148
                                                                                                                                          |                                                                                                                                         
gAATTATTCAAAAAAGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATa                                                                                                                                           <  7:207444/139‑1 (MQ=255)
      ttCAAAAAAGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACtt                                                                                                                                      >  3:93863/1‑138 (MQ=255)
        cAAAAAAGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  4:12895/139‑1 (MQ=255)
         aaaacaGGCAAAATGAAGCCGAATATCGGTGAGCCTCTTCACCAGGCTGATTTAGCCCCCCTGCCTACACAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  4:218593/138‑1 (MQ=255)
         aaaaaaTGAAAAATTAATCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCATTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAATGAACTAGAGAATAAACTTGAt                                                                                                                                   <  8:187271/138‑1 (MQ=255)
         aaaaaaGGAAACATGAAGCTGAGTATCGGTGAGCCTCTTCTCCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCTGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  2:287238/138‑1 (MQ=255)
         aaaaaaGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  5:341160/138‑1 (MQ=255)
         aaaaaaGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  4:319688/138‑1 (MQ=255)
         aaaaaaGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  4:260946/138‑1 (MQ=255)
           aaaaGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAg                                                                                                                                >  4:67032/1‑139 (MQ=255)
            aaaGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGc                                                                                                                               >  6:312832/1‑139 (MQ=255)
                   aaaTGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCATCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAgg                                                                                                                        <  6:97383/139‑1 (MQ=255)
                               tcaTCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAACGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTgagaga                                                                                                            <  7:68914/137‑1 (MQ=255)
                                 aTCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGAt                                                                                                           <  6:139527/138‑1 (MQ=255)
                                        aGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATGAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTg                                                                                                    >  5:324151/1‑138 (MQ=255)
                                        aGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATGAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTg                                                                                                    >  5:318919/1‑138 (MQ=255)
                                        aGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAgg                                                                                                                        >  3:196412/1‑118 (MQ=255)
                                        aGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAgg                                                                                                                        <  4:196412/118‑1 (MQ=255)
                                                cACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACtgtg                                                                                            <  2:38775/138‑1 (MQ=255)
                                                cACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACtgt                                                                                             <  2:1123/137‑1 (MQ=255)
                                                     ggCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   >  4:266092/1‑94 (MQ=255)
                                                     ggCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGAt                                                                                                                                   <  3:266092/94‑1 (MQ=255)
                                                           ttttGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGaaaa                                                                                <  6:28079/139‑1 (MQ=255)
                                                            tttGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGaaaa                                                                                <  1:173999/138‑1 (MQ=255)
                                                             ttGCCCACCTGCCTTCAAAGGAAAGACTTTCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAg                                                                               <  6:178131/138‑1 (MQ=255)
                                                             ttGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAg                                                                               <  8:40354/138‑1 (MQ=255)
                                                                cccACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCAcc                                                                           <  2:305085/139‑1 (MQ=255)
                                                                cccACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCAcc                                                                           <  3:67032/139‑1 (MQ=255)
                                                                cccACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCAc                                                                            <  2:65109/138‑1 (MQ=255)
                                                                cacaacTGCCTTCTAATGAAAGAATTTAATCAATGAAAGAGTCTTTGAATCATCTTATTAAAGAAATAGATAATAAACTTGATCATCTATAAGGCGTTCTTATATATTTTCATGAAACTGTGAAAATAAGAAAAGGAc                                                                            <  2:91467/133‑1 (MQ=255)
                                                                        ccTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATGAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACa                                                                    <  6:324151/138‑1 (MQ=255)
                                                                        ccTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATGAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACa                                                                    <  6:318919/138‑1 (MQ=255)
                                                                            cAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAgg                                                                 <  4:93863/137‑1 (MQ=255)
                                                                                   aaGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAAATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCt                                                        >  6:340986/1‑139 (MQ=255)
                                                                                                aTGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCct                                           >  4:92021/1‑139 (MQ=255)
                                                                                                aTGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCct                                           >  8:109231/1‑139 (MQ=255)
                                                                                                aTGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCct                                           >  3:193434/1‑139 (MQ=255)
                                                                                                 tGAGAGAGTCTTTGATTCAGAGGATTACAGAACTAGAGAATAAACTTGATCAGCTATAAGTCGTTCTGAGAGATTGTCCTGAAACTGTTATAATAAGAAATGCTCCCTATACAATGTGGTTTTCTTATTATGCTTCctg                                          <  2:8379/139‑1 (MQ=255)
                                                                                                 tGACAGAGGCTTTGAATCAGCGGATTAAAGATCTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCctg                                          <  5:78301/139‑1 (MQ=255)
                                                                                                 tGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGATTAAACTTGATCAGCTATAAGGCGTGCTGAGAGATTGTCCTGAAACTGTGAAAATAATAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCctg                                          <  5:314299/139‑1 (MQ=255)
                                                                                                 tGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCctg                                          <  1:82409/139‑1 (MQ=255)
                                                                                                  gACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCctgc                                         >  4:14854/1‑139 (MQ=255)
                                                                                                         gCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAg                                  <  8:243598/139‑1 (MQ=255)
                                                                                                            ttGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTg                               >  1:266655/1‑139 (MQ=255)
                                                                                                             tGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCt                                <  5:312832/137‑1 (MQ=255)
                                                                                                                   aGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGtctc                           >  6:56969/1‑136 (MQ=255)
                                                                                                                   aGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAg                        <  4:160195/139‑1 (MQ=255)
                                                                                                                                  tAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAGAAGTTcttcttctt          >  2:242911/1‑138 (MQ=255)
                                                                                                                                  tAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAGAAGTTcttcttctt          >  1:215364/1‑138 (MQ=255)
                                                                                                                                         tAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAGAAGTTCTTCTTCTTGAAGCttt  >  3:181262/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GAATTATTCAAAAAAGGAAAAATGAAGCTGATTATCGGTGAGCCTCTTCACCAGGCTGATTTTGCCCACCTGCCTTCAAAGGAAAGACTTGCAGCAATGACAGAGGCTTTGAATCAGCGGATTAAAGAACTAGAGAATAAACTTGATCAGCTATAAGGCGTTCTGAGAGATTGTCCTGAAACTGTGAAAATAAGAAAAGCACCCTATACAAGGTGCTTTTCTTATTATGCTTCCTGCTGGTTAGCTGTCTCCAGAAGTTCTTCTTCTTGAAGCTTT  >  CP006881/994873‑995148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑