Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,653,749 A→G 6.9% E198G (GAA→GGA)  U712_08495 → GTP‑sensing transcriptional pleiotropic repressor CodY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,653,7490AG 6.9% 272.1 / 7.2 87E198G (GAA→GGA) U712_08495GTP‑sensing transcriptional pleiotropic repressor CodY
Reads supporting (aligned to +/- strand):  ref base A (36/45);  new base G (3/3);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAATCCTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCA  >  CP006881/1653611‑1653883
                                                                                                                                          |                                                                                                                                      
tGGAAATCCTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGa                                                                                                                                        <  8:18799/139‑1 (MQ=255)
   ctaTCCTAAGAGAAAAAGCATATGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTTTCAGGTCGCTTTCTTACATTTAGCTTGTAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGaatt                                                                                                                                     <  4:72898/137‑3 (MQ=255)
   aaaTCCTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAgg                                                                                                                                     <  3:73250/139‑1 (MQ=255)
   aaaTCCTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAgg                                                                                                                                     <  7:131191/139‑1 (MQ=255)
        cTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGTTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTCAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGtcttc                                                                                                                                >  4:40016/1‑139 (MQ=255)
        cTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTCGACGGAAATGa                                                                                                                                        <  6:209268/131‑1 (MQ=255)
        cTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTCGACGGAAATGa                                                                                                                                        >  5:209268/1‑131 (MQ=255)
        cTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGGAGGtcttc                                                                                                                                >  3:85572/1‑139 (MQ=255)
             agaAACAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCttgtt                                                                                                                           <  6:20951/139‑1 (MQ=255)
                aaaaGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCa                                                                                                                        <  6:359354/139‑1 (MQ=255)
                 aaaGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGc                                                                                                                         >  1:231593/1‑137 (MQ=255)
                 aaaGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGc                                                                                                                         >  2:277272/1‑137 (MQ=255)
                    gCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTa                                                                                                                    <  1:1895/139‑1 (MQ=255)
                    gCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTa                                                                                                                    <  1:277272/139‑1 (MQ=255)
                       gaagaaATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGTTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTaaaa                                                                                                                 >  8:358460/1‑139 (MQ=255)
                                  agagGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCgt                                                                                                      >  3:242745/1‑139 (MQ=255)
                                        agcaagaagcaaAGCTGTCGTACAAATGGTTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGc                                                                                                <  7:358460/139‑1 (MQ=255)
                                          caagaagcaaAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCAt                                                                                              <  6:356310/139‑1 (MQ=255)
                                                gcaaAGCTGTCGTACAAATGGCTATCAGCTCGGTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCg                                                                                        <  3:115423/139‑1 (MQ=255)
                                                    aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCt                                                                                    >  4:170103/1‑139 (MQ=255)
                                                         tCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGGAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTTTTAt                                                                               >  4:316697/1‑139 (MQ=255)
                                                                     cTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCTCATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACt                                                                   <  5:328319/139‑1 (MQ=255)
                                                                        tCAGCTCGCTTTCTTACAGTGTGGTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAATATTGCTGACCGTGTCTGCATTACCCGTTCTGTTATTGTGAACGCACTCa                                                                 <  8:360167/138‑1 (MQ=255)
                                                                          aGCTCGCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTTAAATTTCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGa                                                               <  2:231593/138‑1 (MQ=255)
                                                                          aGCTCGCTTTCTTACAGGGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGa                                                               <  5:171666/138‑1 (MQ=255)
                                                                              cGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCt                                                          >  1:76213/1‑139 (MQ=255)
                                                                               gCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGc                                                                                                                         <  2:90557/75‑1 (MQ=255)
                                                                               gCTTTCTTACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGc                                                                                                                         >  1:90557/1‑75 (MQ=255)
                                                                                     ttACAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgc                                                   >  2:34121/1‑139 (MQ=255)
                                                                                        cAGTGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                 <  1:34121/138‑1 (MQ=255)
                                                                                         aGTGAGCTTGACGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                               <  1:147480/139‑1 (MQ=255)
                                                                                           tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACg                                                                       >  7:319887/1‑113 (MQ=255)
                                                                                           tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACg                                                                       >  7:102401/1‑113 (MQ=255)
                                                                                           tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACg                                                                       <  8:102401/113‑1 (MQ=255)
                                                                                           tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACg                                                                       <  8:319887/113‑1 (MQ=255)
                                                                                           tGAGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                             >  3:203083/1‑139 (MQ=255)
                                                                                           tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCttgtt                                                                                                                           <  3:333190/61‑1 (MQ=255)
                                                                                           tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCttgtt                                                                                                                           >  4:333190/1‑61 (MQ=255)
                                                                                             aGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                 >  3:200546/1‑133 (MQ=255)
                                                                                             aGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                 <  4:200546/133‑1 (MQ=255)
                                                                                             aGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                           >  8:159173/1‑139 (MQ=255)
                                                                                             aGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                           >  1:290590/1‑139 (MQ=255)
                                                                                             aGCTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGCTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGATGGAGAGCGCCTgtgt                                           >  1:220404/1‑139 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCa                                                                                                                        <  6:318537/62‑1 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCa                                                                                                                        >  5:318537/1‑62 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCgtgt                                                                                                    <  7:87273/82‑1 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCgtgt                                                                                                    >  8:87273/1‑82 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGTGCCGgtgt                                           >  8:359295/1‑139 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                           >  2:108520/1‑139 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                           >  3:118081/1‑139 (MQ=255)
                                                                                             aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                           >  5:79402/1‑139 (MQ=255)
                                                                                               cTTGAAGTAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTa                                         >  1:161045/1‑139 (MQ=255)
                                                                                               cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTAc                                                                                           >  5:153445/1‑89 (MQ=255)
                                                                                               cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTAc                                                                                           >  1:293731/1‑89 (MQ=255)
                                                                                               cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTAc                                                                                           <  6:153445/89‑1 (MQ=255)
                                                                                               cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTAc                                                                                           <  2:293731/89‑1 (MQ=255)
                                                                                                  gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGGAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACTCACTCAGAAAGCTGGAGAGCGCCGGTGTTAt                                        <  6:152737/137‑1 (MQ=255)
                                                                                                  gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                       <  6:335010/138‑1 (MQ=255)
                                                                                                  gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                       <  3:170103/138‑1 (MQ=255)
                                                                                                       aaTTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc                                  <  3:40016/138‑1 (MQ=255)
                                                                                                         ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCg                                                                                        <  7:95035/82‑1 (MQ=255)
                                                                                                         ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCg                                                                                        >  8:95035/1‑82 (MQ=255)
                                                                                                         ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc                                  >  5:35948/1‑136 (MQ=255)
                                                                                                             gCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCa                           >  7:326827/1‑139 (MQ=255)
                                                                                                              cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAt                          >  1:142724/1‑139 (MQ=255)
                                                                                                               acaTTTTTGAGGAGCTTGACGGAAAGGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGACAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAtt                         <  4:52523/139‑1 (MQ=255)
                                                                                                                caTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt                                                                                      <  1:327903/77‑1 (MQ=255)
                                                                                                                caTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt                                                                                      >  2:327903/1‑77 (MQ=255)
                                                                                                                   ttttGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAgg                      >  7:16913/1‑138 (MQ=255)
                                                                                                                    tttGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGa                     <  1:108520/138‑1 (MQ=255)
                                                                                                                        aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATAAGGAATGa                 >  8:233462/1‑138 (MQ=255)
                                                                                                                        aggagCGTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                <  2:199480/139‑1 (MQ=255)
                                                                                                                         ggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGTGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaaa               <  7:359295/139‑1 (MQ=255)
                                                                                                                          gagCTTGACGGAAATGGAGGTCTTCTTGTTGCAATTAAAATTGCTGACAGTGTCGGCATTACGCTTTCTGTTATTGTGAACGCACTTAGATAGTTTTAGATCTCCGGTGTTATCGAGTCTAGATCATTAGGATTGAAAg              <  4:85572/139‑1 (MQ=255)
                                                                                                                          gagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAg              <  2:142724/139‑1 (MQ=255)
                                                                                                                             cTTGACGGAAATGGAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAAGGAAAGGTa           >  1:152882/1‑139 (MQ=255)
                                                                                                                             cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTa                        <  5:30541/126‑1 (MQ=255)
                                                                                                                             cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTa                        >  6:30541/1‑126 (MQ=255)
                                                                                                                             cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTa           >  3:130093/1‑139 (MQ=255)
                                                                                                                             cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTa           >  8:82321/1‑139 (MQ=255)
                                                                                                                                gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTAc          <  4:118081/137‑1 (MQ=255)
                                                                                                                                gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTAc          <  6:79402/137‑1 (MQ=255)
                                                                                                                                gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTAc          <  6:4500/137‑1 (MQ=255)
                                                                                                                                gACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACt         <  4:203083/138‑1 (MQ=255)
                                                                                                                                      aaTGGAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa  <  3:316697/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa  <  2:76213/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa  <  4:242745/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa  <  7:159173/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTACTTATATCa  <  8:326827/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATAAAAGGTACTTATATCa  <  5:86297/139‑1 (MQ=255)
                                                                                                                                      aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGAGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa  <  5:267903/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TGGAAATCCTAAGAGAAAAAGCAGAAGAAATTGAAGAGGAAGCAAGAAGCAAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCA  >  CP006881/1653611‑1653883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑