| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,672,787 | G→T | 21.8% | M1M (ATG→ATT) † | U712_08615 → | Flagellar biosynthesis protein flhF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,672,787 | 0 | G | T | 21.8% | 85.5 / 7.7 | 56 | M1M (ATG→ATT) † | U712_08615 | Flagellar biosynthesis protein flhF |
| Reads supporting (aligned to +/- strand): ref base G (21/21); new base T (10/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTCGCCGCCTGTCAGAATGTATGTGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCTCGTCAACAAGCC > CP006881/1672657‑1672921 | tGCTCGCCGCCTGTCAGAATGTATGTGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATAGGAGTGGTGGATATTTAATTAAAAT‑aa > 4:361712/1‑138 (MQ=255) cTCGCCGCCTGTCAGAATGTATGTGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑aaaa > 5:340198/1‑138 (MQ=255) gAATGTATGTGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTgctgct < 2:244912/139‑1 (MQ=255) tatgtGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAAt > 6:339133/1‑139 (MQ=255) tatgtGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTAATTCTTCTTCAAt > 6:172686/1‑139 (MQ=255) tgtgAAGCAGCTACTGGACCGGTACTTCCCTGAGGTTCCGGTAGTCTCTTATAACGACCTTGAGGCGAATGTTGAGGTACAGAGCATTGGAGTGGGGGAGTTTTTATGTAATT‑TAAAACAGTTTCTGGTGGTGCAATg > 2:53104/1‑138 (MQ=255) aaGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGTTATTTAATTAAAAT‑AAAAAAATTTAATGGTGCTTCaaagaaag > 3:157308/1‑138 (MQ=255) aaGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGGTTTTTTATGAAAAT‑AAAAAAATTTAATGATGCTTGAATGc > 5:222515/1‑135 (MQ=255) aaGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCaaga < 2:52050/139‑1 (MQ=255) aaGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCa < 4:260271/136‑1 (MQ=255) aaGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCa > 3:260271/1‑136 (MQ=255) cTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATTAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGc > 1:286709/1‑139 (MQ=255) cTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCgg < 5:262111/138‑1 (MQ=255) cTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCgg > 6:262111/1‑138 (MQ=255) cGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCtcat < 5:339133/139‑1 (MQ=255) cGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCtcat < 5:172686/139‑1 (MQ=255) aCTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGGTATTTTATTAAAAT‑AAAAAAATTTTATGCTGCGTGAATGCAAGGAGCGGCCCGTCtcatcct > 6:309440/1‑136 (MQ=255) cTGATCTTACGGTACTCTCTTATAACGAACTGGAGGCGAGTGTTTAGGGACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAagag < 6:133861/139‑1 (MQ=255) agacagccGTTACTCTATTATAACGAACTTGAGGCTAATGTTGAGGTAAAAAGAATTGGAGTGGTGTATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGtt < 4:286984/133‑1 (MQ=255) atattgcGGTATTGTCTTATAACGAACTTGAGGCGATTGTTGAGGTACAAAGCATTGTAGTTGTGTATATTTAATTAAATT‑AAAATAATTTACTGCTTCTTCAATGCAAGTAGCGGCACTTCTCATCAGAAAAGTGtt < 6:270984/132‑1 (MQ=255) agcTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGTATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGtt < 6:41535/136‑1 (MQ=255) accTTCCGGTACACTCTTATAACGAACTTCCGGCTAATGTTGCGGTACAAAGCATTGGAGTGGGGGATATTTAATTAAAAT‑AAAAAAATTAACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGtt < 6:36637/136‑1 (MQ=255) aTCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATTAAAAT‑AAAAAAATTTAATGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTa > 3:26578/1‑139 (MQ=255) aTCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGtt < 3:147567/138‑1 (MQ=255) aTCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑‑AAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGtt < 3:310883/137‑1 (MQ=255) aTCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATATAAAAAAAAA‑AAAAAAAATTACAGCTGCATCAATGATACAAGCCCCACAACTCGACAGAAAAAAGTTa > 5:62809/1‑139 (MQ=255) ccGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTa < 8:6862/138‑1 (MQ=255) cGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTAAAGCTGCTTCAATGCAAGAAGCGGCACGTCTCATCAGAAAAGAGTTAGGTAAt > 3:288468/1‑139 (MQ=255) ggTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAAt > 3:328586/1‑138 (MQ=255) gTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGa < 6:340198/139‑1 (MQ=255) ctctctTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCTTTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAg < 3:361712/138‑1 (MQ=255) ctctctTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAg < 5:359714/138‑1 (MQ=255) ttATATCGAACTTGAGGTGAATGTTGATGTACAAAGCATTGGAGTGGTGGTTTTTTAATGAATAT‑AAAAAAATTTACTGCTGCTTCAATGAAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGt < 4:157308/137‑1 (MQ=255) ttATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGTTATTTTTTTAAAAT‑AAAAAAATTTTAAGCTGCTTCAATGCAATAACCGGCGCTTCTCATCAGAAAATAGTTAGGTTATGAAGCgggga > 5:316453/1‑136 (MQ=255) ttATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGTTATTTAAATAAAATAAAAAAAATTTAAT‑CTGCTTAAATGCAAGAAGCGGCACTTCTCAAAATAAAAGAGTTATGTTATGAAGctgg > 3:286984/1‑134 (MQ=255) ataACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGt < 4:328586/135‑1 (MQ=255) taACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTAATGCTGCTTCAATGCAAGAAGCGGCACTTGTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCatat > 2:252679/1‑139 (MQ=255) aaCTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGTTATTTTATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAATCGGGCCTTCTCATCAGATAAGATTTATGTAATTAAGCGGTCAAATTAAAt > 4:45127/1‑139 (MQ=255) aaCTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTATCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAAt > 7:296828/1‑139 (MQ=255) cTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATTAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCAAATCAGAAAATAGTTTGGTAATGAAGCGGTCATATTAAAt > 1:118995/1‑137 (MQ=255) cTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑TAAAAAATTTTATTCTGCTTCTATGCAAGAAGCGGCACTTGTGATCTGAAAAGAGTTATGTTATGTAGCGGTCATATTAAATTc > 6:130052/1‑139 (MQ=255) cTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCTTCAGAAAAGAGTTAGGTAATGAAGCGGGCATATTAAATTc > 7:155423/1‑139 (MQ=255) aaTGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCAAATTAAATTCGAAAAAAAt > 2:73531/1‑139 (MQ=255) aaTGTTGAGGTACAAAGCATTGGAGTGGTGGATAATTTATTATTAT‑AAAAAAATTTTATGCAGCTTCAATTCAAGAAGCGGCAAATATCAACATAAAAGACATAGGGAATTAAGCGGTGATATTAAATTAGAAAAAAAt > 1:243898/1‑139 (MQ=255) tGTTGAGGTACAAAGCATTGGAGTGGTGGGTATTTAATTAAAAT‑AAAAAAATTTTCTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTTATGAAGCGGTCATATTAAATTCGAAAAAAAtt > 5:27762/1‑138 (MQ=255) aaaaGCATTGGAGTGGTTGATATTTAATCAAAAT‑AAAAAAATTTAATGATGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGaaa < 5:315998/138‑1 (MQ=255) cATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGt > 5:180279/1‑139 (MQ=255) tGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGATTTAGGTAATGAAGCGGGCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTg > 2:12080/1‑139 (MQ=255) tGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTg > 7:179286/1‑139 (MQ=255) tGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACAGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTTGGGAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAAAGGTTTg > 5:340356/1‑139 (MQ=255) caggggtgcATATTTAATGAAAAA‑AAAAAAATTTACGGCTCCTTCAAGGCAAGCCGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAACGCGAAAATGGTTTGGc < 6:316453/135‑1 (MQ=255) tggtggATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCTc < 1:252679/139‑1 (MQ=255) gtggATATTTTATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGGCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCGCGt > 4:20964/1‑139 (MQ=255) aaTGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAagag < 1:136636/64‑1 (MQ=255) aaTGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAagag > 2:136636/1‑64 (MQ=255) aaTGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCTCGTCAACAAGcc < 1:12080/138‑1 (MQ=255) | TGCTCGCCGCCTGTCAGAATGTATGTGAAGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGCATTGGAGTGGTGGATATTTAATGAAAAT‑AAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGTCATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCTCGTCAACAAGCC > CP006881/1672657‑1672921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |