Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,951,390	C→G	19.9%	K227N (AAG→AAC)	U712_09660 ←	D‑alanyl‑D‑alanine carboxypeptidase dacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,951,390	0	C	G	19.9%	110.5 / 4.5	70	K227N (AAG→AAC)	U712_09660	D‑alanyl‑D‑alanine carboxypeptidase dacC
Reads supporting (aligned to +/- strand):  ref base C (20/31);  new base G (12/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 2.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAGACAGAAATCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTG  >  CP006881/1951254‑1951526                                                                                                                                         |                                                                                                                                          agacagcagaAATCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCtt                                                                                                                                         <  5:75667/133‑1 (MQ=255)    agacagaAATCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTAt                                                                                                                                      <  4:16838/139‑1 (MQ=255)          aaaTCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTTATTCGTTCTTTATCGTta                                                                                                                                 >  8:157359/1‑138 (MQ=255)          aaaTCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTtat                                                                                                                                >  6:59988/1‑139 (MQ=255)          aaaTCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTta                                                                                                                                 >  8:236928/1‑138 (MQ=255)            aTCCATTCCTTTGTCTTGTTTGCGTCAACAGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCAATGCTCTCTTTCGATTGTGAGGTGTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCGTTATCGttttt                                                                                                                               >  6:144910/1‑135 (MQ=255)                  tCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTTCATCGGTCTTTATAGTtttatatgtct                                                                                                                        >  6:75667/1‑134 (MQ=255)                  tCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTTTCGTTATATAATCgg                                                                                                                        >  1:190804/1‑139 (MQ=255)                      ttGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGGATCGGTGTTTATCGTTTTATAATATGtttt                                                                                                                    >  5:136607/1‑139 (MQ=255)                       tGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGCATGGTTAGTTTTGATTATATTATAGGttt                                                                                                                     >  4:105426/1‑137 (MQ=255)                             gTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGga                                                                                                                <  2:21765/136‑1 (MQ=255)                             gTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGga                                                                                                                <  6:297367/136‑1 (MQ=255)                              tttGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATGGTTCTTTTTAGTTTTATAATCGGTTTTCGtagaga                                                                                                            >  7:58799/1‑139 (MQ=255)                                tGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGCATCGGTGTTTTTAGTTATTTAATCGTGTTTAGgagagt                                                                                                            >  4:6793/1‑136 (MQ=255)                                  cggcAACCGGTCCGCTCCATTCAATTTTATTCTGGTTTGGTCCAGGCTCTATTTCGTTTGTGAGGTGTTTCTCTTAACGGGCAGCAGTGGTTTTAGCATCGTTCTTTATCGTTATATAATGGGTTTTCGGAGatacccc                                                                                                        <  4:224646/136‑4 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTGCATCGTTCTTTTTCGTTATATAATCGGTTTTCGGAGagacaga                                                                                                        >  5:151226/1‑138 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGGATCGTTGTTTATCGTTATATTATCGGGTTTCGGAGagacagcc                                                                                                       >  8:28261/1‑137 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGGATCGTGCTTTATGGTTAGATAATCGGGTTTCGGAGagccagac                                                                                                       >  5:151225/1‑139 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGagacaga                                                                                                        >  7:111537/1‑138 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGagacaga                                                                                                        <  8:111537/138‑1 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGGAAGCTTGTTTTTGGTTATATAATACGTTTTCGGAGagacagc                                                                                                        >  2:214113/1‑137 (MQ=255)                                   gTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTAGCATCGGTGTTTATCGTTATATAATCGGTTTTAGGAGagccagac                                                                                                       >  8:7444/1‑139 (MQ=255)                                         cGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCTTTATATAATCGGTTTTCGGAGAGACAGACACGGCa                                                                                                 <  2:190804/139‑1 (MQ=255)                                           gTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATGGTGTGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCAGCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAgg                                                                                               <  1:265841/139‑1 (MQ=255)                                            tACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACGGACACGGCAGGc                                                                                              <  3:151724/139‑1 (MQ=255)                                                    cTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTcccc                                                                                      <  3:129794/139‑1 (MQ=255)                                                     ttCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTTCATCGTTCTTTATCGTTTTATAATCGGTTTTCGGAGAGACAGCCACGGCCGGCTCTTCCCct                                                                                     >  4:277862/1‑139 (MQ=255)                                                               aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTGCATCGTTGTTTTTCGTTATATTTTGGGGTTTTGGTGAGACAGACACGGCAGGGGCTTCCCCGTGTTTTTTAt                                                                           >  6:95070/1‑139 (MQ=255)                                                               aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTGCATAGTTGTTTATAGTTATATAATAGGTTTTGGGAGAGACAGACACGGCAGGGTATTCCCCTTGTTTTTTAt                                                                           >  3:306443/1‑139 (MQ=255)                                                               aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATAGTTATATTATCGGTTTTCGGAGAGACAGACACGGCAGGGTCTGCCCCTACGTTTTGAt                                                                           >  7:59612/1‑139 (MQ=255)                                                               aTCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATAGTTCTTTATAGTTATATAATATGTTTTAGGAGAGACAGACACGGCAGGCTCTTCCCCAACATTTTGAt                                                                           >  2:67892/1‑139 (MQ=255)                                                                  gtgtTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTATCATCGTTATTTATAGTTTTATAATCGGTTTTCGGAGAGACAACCACCGCACGCGGTTACCCTTAtttttttgttt                                                                        >  7:242793/1‑133 (MQ=255)                                                                           ccATGCTCTCTTTCTATTGTGAGGCCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCTTTATATAATCGGTTTTCGGAGAGACACACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGAcc                                                                <  3:58422/138‑1 (MQ=255)                                                                           ccATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGAcc                                                                <  7:252577/138‑1 (MQ=255)                                                                            cATGCTCTCTTTCGATTTTGAGGTCTTGCACTTAACCGCCAGCAGTCTTTTTAGAATCGTTCTTTAGCGTTATATAATCGGTTTGCGTAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑Tc                                                             <  5:183289/139‑1 (MQ=255)                                                                              tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGtttt                                                                                                                    <  8:238249/83‑1 (MQ=255)                                                                              tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGtttt                                                                                                                    >  7:238249/1‑83 (MQ=255)                                                                              tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGCCTCGTTGTTTATGGTTTTTTAATCGGTTTTGGGTGTGACACACCCGGCCCGGGCTTGCCCGTCGTTTTGTTTTTGGGGGGAC‑Tcaa                                                           >  6:199148/1‑139 (MQ=255)                                                                              tGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGCCTAGGTGTTTATAGGTATATTATCGGTTTTAGGAGAGACAGACACGGCCGGCGCCTGCCCGTGGTTTTGTTTTTGGGTGGCC‑tga                                                            >  4:83029/1‑136 (MQ=255)                                                                                   tctTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAAt                                                       >  5:128591/1‑138 (MQ=255)                                                                                       tcgctggtgCGGTCTTTCTCTCAACCGGCCGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACACACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATcac                                                    <  3:6793/131‑1 (MQ=255)                                                                                       tCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATcac                                                    <  6:131252/137‑1 (MQ=255)                                                                                       tCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATca                                                     <  7:236928/136‑1 (MQ=255)                                                                                       tCGATTGTGAGGGCTTTCTCTGAACGGGCAGCAGGCGTTTTAGCATCGTTATTAATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATcaca                                                   <  7:349353/138‑1 (MQ=255)                                                                                          aTTTTGAGGGCTTTCTCTGAACCGGCAGCTGTCGTTTTAGCATCGTTGTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  3:83029/139‑1 (MQ=255)                                                                                          aTTGTGCGTTCTTTCTCTTAACCGGCAGCAGTCGTTTTAGCATGGTTCTTTATCGTAATATAATCGGTTTTCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  8:58799/139‑1 (MQ=255)                                                                                          aTTGTGCGGTCTTTCTCTCACCCGGCAGGAGTCGTTTTAGCATCGTTCTTTATGGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGGGACC‑TCAACAATCACAGTGc                                               <  5:144910/139‑1 (MQ=255)                                                                                          aTTGTGAGGTCTTTCTCTGAACCGGCAGCTGTCGTTTTAGCATCGTTCTTTATCGTTATATAAGCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  3:72479/139‑1 (MQ=255)                                                                                          aTTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGACACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  5:59988/139‑1 (MQ=255)                                                                                          aTTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTTGCATAGTTGGTTATGTGTATATAATCGGTTTTGGGAGAGACAGAAACCGCCGGCGCTTCCCCGTGTTTTTGTTTTTGGGTGGGC‑GCAACAATCACCGTGc                                               >  8:168902/1‑139 (MQ=255)                                                                                          aTTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTGTTAGCATCGTTCTTTTTCGTTTTATTAACGGTTTTAGGAGAGACAGACACGGGAGGCGCTTCCCCGTCTTTTTGATTTGGGGGGACCGGCAAC‑ATCACAGttc                                               >  8:6944/1‑137 (MQ=255)                                                                                          aTTGTGAGGTCTTTCTCTCAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  4:313719/139‑1 (MQ=255)                                                                                           ttGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGAATGGTTCTTTATCGTTATAAAATCGGTTTTCGGAGAGACATACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  6:136607/138‑1 (MQ=255)                                                                                             gtgtgttCTTTCTCTGCACCGGCCGCAGTCGTTTTAGCATCGTTCTTTAGCTTTATATAATCGTTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCt                                             <  7:157359/132‑1 (MQ=255)                                                                                             gtgAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTTGCATCGTTGTTTTTAGTTTTATTATCGGTTTTCGGAGAGACAGACACGGCAGGCTCGTCCCCGTCTTTTTGATTTTGGGTGACC‑TCCACAAAAAAAGTGCCTg                                            >  4:146816/1‑139 (MQ=255)                                                                                             gtgAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCTTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCt                                             <  4:306443/138‑1 (MQ=255)                                                                                                  tgcTTTCTCTGACCCGGCAGCTGTCTTTTTTGAATCGTTTTTTTTCTTTATATATTGGTTTTTCGGAGACACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGcatca                                       <  4:54375/137‑1 (MQ=255)                                                                                                     tttCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTGTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAg                                    >  3:87463/1‑139 (MQ=255)                                                                                                     tttCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATAGTTATATAATAGGTTTTCGGAGAGACAGACACGGCCGGCTCTTCCCCTGCTTTTTGTTTTGGGGTGGCC‑TGCACAATCACAGTGGCTGcatcattg                                    >  2:286983/1‑137 (MQ=255)                                                                                                         tctGATCCGGCAGATGAAGTTTTCGAATCGTTGTTATTCGTAAATTAATGTCTTGTGGGTGAGCCTCACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCt                                 <  2:346637/138‑1 (MQ=255)                                                                                                             aaCCGGCAGCTGTGGTTTTAGCTTCCTTCTTTCTGGTATTAACATCGGTTTTGGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGt                             <  6:16836/138‑1 (MQ=255)                                                                                                                 ggCAGCTGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGga                        <  7:7444/139‑1 (MQ=255)                                                                                                                   ctgcagTCGTTTTTGAATCGTTCTTTATGGTTATATACTCGGTTTTCGGAGAGACAGCCACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                      <  3:73344/137‑1 (MQ=255)                                                                                                                   cagcagTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGgaga                      <  1:67892/139‑1 (MQ=255)                                                                                                                      ccgaggTTTTAGCATCGTTCTTTTTGGTAATAAAAGCGGGTTGCGGCGACACACCCACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGc                   <  1:314352/134‑1 (MQ=255)                                                                                                                               gttattCGTTATTTTTCGTTTTATAATGGGTTTTGGGTTTTACAGACACGTCAGGCTCTTCCCCTTGTTTTTGATTTGGGGTGACC‑TCAACAATCAGAGTGCCTGCATCATAGTCTTCGTTTGTAGACGCTGTTagag           <  7:46387/133‑1 (MQ=255)                                                                                                                                       ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               >  2:290932/1‑94 (MQ=255)                                                                                                                                       ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGc                                               <  1:290932/94‑1 (MQ=255)                                                                                                                                       ttCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTg  >  7:83594/1‑139 (MQ=255)                                                                                                                                       ttCTTTATCGTTATATAAACGCTTTTAGCAGACACAGACACGGCAGGCTCGTCCGCTTCTTGTTGATTTGGGGTGAAC‑TCAACAAACACAGTCCCTGAATCATAGTCTAAGTTTGGAGACGCTGTTTGCGCGGAGATTg  >  8:157156/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                          CCCAGACAGAAATCCATTCCTTTGTCTTGTTTGCGTCAACCGGTACGCTCCCTTCAATTGTAATCGTGTTTGTTCCATGCTCTCTTTCGATTGTGAGGTCTTTCTCTGAACCGGCAGCAGTCGTTTTAGCATCGTTCTTTATCGTTATATAATCGGTTTTCGGAGAGACAGACACGGCAGGCTCTTCCCCTTCTTTTTGATTTGGGGTGACC‑TCAACAATCACAGTGCCTGCATCATAGTCTTCGTTTGGAGACGCTGTTAGAGCGGAGATTG  >  CP006881/1951254‑1951526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑