Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,062,972 G→T 6.8% H298N (CAT→AAT)  U712_10210 ← 2‑oxoglutarate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,062,9720GT 6.8% 190.0 / 3.8 59H298N (CAT→AAT) U712_102102‑oxoglutarate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base T (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

TCGCGTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCAAGACATTCA  >  CP006881/2062838‑2063109
                                                                                                                                      |                                                                                                                                         
tCGCGTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTAt                                                                                                                                       <  5:346073/139‑1 (MQ=255)
 cgcgTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGTTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTAtt                                                                                                                                      >  8:295914/1‑139 (MQ=255)
 cgcgTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTAt                                                                                                                                       <  7:255614/138‑1 (MQ=255)
    gTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGTTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGa                                                                                                                                   <  7:295914/139‑1 (MQ=255)
     tttCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGAc                                                                                                                                  <  6:36027/139‑1 (MQ=255)
       tCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGTAGCTCCCGATTCGCCGCCATATGGTTTTTGGCAt                                                                                                                                >  4:329698/1‑139 (MQ=255)
       tCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATTGt                                                                                                                                         <  3:330893/130‑1 (MQ=255)
       tCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATTGt                                                                                                                                         <  3:363871/130‑1 (MQ=255)
       tCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATTGt                                                                                                                                         >  4:363871/1‑130 (MQ=255)
       tCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATTGt                                                                                                                                         >  4:330893/1‑130 (MQ=255)
              ccGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGt                                                                                                                         <  4:18616/139‑1 (MQ=255)
                 ctctTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGCCATCCCCCGTc                                                                                                                        >  2:146800/1‑137 (MQ=255)
                 ctctTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGAGTTTGTTTCAGCGTCTTGCAGCTCCCGATTCGCCCCCATATGCTAGTGGCGATCCACCGTccg                                                                                                                      >  4:230530/1‑138 (MQ=255)
                      gtgCTGCCTTCACCAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTCATGCGCGCTGATTTTGTTTCAGCTTCTTGAAGCTCCCGAGTCGCCCCCAAGTGGGATTTGACTTCCCCGGGccgtccgt                                                                                                                 >  8:205823/1‑139 (MQ=255)
                         ctgcCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGc                                                                                                              >  1:13905/1‑139 (MQ=255)
                            ccgtCAACAATCGGATTGATAAATGCCAAGGGGCTCGGGTGGGTCGCTACCGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCCTCCGTAGCTg                                                                                                            <  7:205823/135‑1 (MQ=255)
                            ccTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTTGTTGACATCCCCCGTCCATCCGTCGCTGa                                                                                                           >  8:276997/1‑139 (MQ=255)
                            ccTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGGCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCt                                                                                                             <  5:276134/137‑1 (MQ=255)
                              ttCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATccc                                                                                                                             >  2:89170/1‑119 (MQ=255)
                              ttCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATccc                                                                                                                             <  1:89170/119‑1 (MQ=255)
                                caacaaTCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCCAATGGTATTTGACATCCCCCGTCCATCCGTCGCTGGTTc                                                                                                        >  4:251795/1‑138 (MQ=255)
                                     aTCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  <  7:276997/139‑1 (MQ=255)
                                     aTCGGATTGATAAATTCCAAGTGGCTCGGGGTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  <  8:35335/139‑1 (MQ=255)
                                      tCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATg                                                                                                   >  3:292153/1‑137 (MQ=255)
                                      tCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGa                                                                                                 <  3:251795/139‑1 (MQ=255)
                                        ggATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGt                                                                                                                         <  4:144398/113‑1 (MQ=255)
                                        ggATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGt                                                                                                                         >  3:144398/1‑113 (MQ=255)
                                                aaaTTCCAAGTGGCTCGTGTTGTTCGCTAACGTAATGCGCGCTGTTTTTGTTTCAGCGTCTGGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGa                                                                                        <  3:163642/138‑1 (MQ=255)
                                                       aaGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACaa                                                                                <  2:306734/139‑1 (MQ=255)
                                                        aGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCACATGGTATTTGACCTCCCCCGGCCATCCGTCGCTGAGTCCACTGGATCCCTCAGACGGttc                                                                                  >  1:70597/1‑134 (MQ=255)
                                                             cTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCtt                                                                          >  3:106152/1‑139 (MQ=255)
                                                             cTCGGGGTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTGGCCCCCAAATGGTATTTGACATCCCCCGGCCGTGCGTAGCTGCTTCCAATGGATCCCGCTGACGGGACAAGagagt                                                                          >  8:354499/1‑135 (MQ=255)
                                                              tCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  <  7:261829/114‑1 (MQ=255)
                                                              tCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  >  8:261829/1‑114 (MQ=255)
                                                                            aaCGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCt                                                           >  5:323897/1‑139 (MQ=255)
                                                                             aCGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTg                                                          <  2:13905/139‑1 (MQ=255)
                                                                                       cgcTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCGCCCGTCCCTCCGTAGCTGATTCCAATGGAGCCCTCAGCCGGTACAAGAGGTTGGTGCGGCGCAGGCTGGatgtca                                                   >  8:79856/1‑131 (MQ=255)
                                                                                       cgcTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  <  2:259478/89‑1 (MQ=255)
                                                                                       cgcTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATgg                                                                                                  >  1:259478/1‑89 (MQ=255)
                                                                                       cgcTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCa                                                   >  4:218962/1‑136 (MQ=255)
                                                                                           gATTTTGTTGCAGCGTCTTGAAGCTCCCGATTCTCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGagaaga                                            <  2:70597/139‑1 (MQ=255)
                                                                                                     cAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCAt                                   <  4:292153/138‑1 (MQ=255)
                                                                                                     cAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCAt                                   <  3:230530/138‑1 (MQ=255)
                                                                                                      aGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGCCATCCCCCGTCCATCCGTAGCTGATTACAATGGATCACTCAGACGGTACAAGATCTGTGTTCGGCGCATGCTGGAATTCAGAGAATATGATTTCAttc                                 >  5:19780/1‑137 (MQ=255)
                                                                                                      aGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCATTGGATCCCTCAGACGGTACAAGAGCTTTGTTCGGGGCTTGCTGGATTTCAGAGAATATGATTTCGTAc                                 >  7:317335/1‑139 (MQ=255)
                                                                                                      aGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGAAGCTGATTCCAATGGATACCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGATTTCAGAGAAGATGATTACATAc                                 >  1:356285/1‑139 (MQ=255)
                                                                                                                    cccGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGcac                   <  3:218962/139‑1 (MQ=255)
                                                                                                                    cccGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGca                    >  4:54020/1‑138 (MQ=255)
                                                                                                                    cccGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGGTTGCCGAGca                    >  8:179553/1‑138 (MQ=255)
                                                                                                                     ccGATTCGCCCCCAAATGGTATTTGCCATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCTGAGAAGATGATTTCATACGGTTTGCCGAGcaca                  >  5:184579/1‑139 (MQ=255)
                                                                                                                     ccGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGt                                                                       >  3:192837/1‑86 (MQ=255)
                                                                                                                     ccGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGt                                                                       <  4:192837/86‑1 (MQ=255)
                                                                                                                            gCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCa           <  7:79856/139‑1 (MQ=255)
                                                                                                                            gCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCa           <  1:146800/139‑1 (MQ=255)
                                                                                                                            gCCCCCAAAAGGTAGTTGACCTCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCa           <  6:15976/139‑1 (MQ=255)
                                                                                                                             cccccAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGa                                                                                        >  2:289360/1‑61 (MQ=255)
                                                                                                                             cccccAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGa                                                                                        <  1:289360/61‑1 (MQ=255)
                                                                                                                                   aaTGGTATTTGACATCCGCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCAAGACAtt    <  3:329698/139‑1 (MQ=255)
                                                                                                                                     tGGTATTTGACATCCCCCGTCCATCCGAAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCAAGACATTCa  <  3:328454/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
TCGCGTTTCCTGTGCCGCTCTTGTGCTGCCTTCAACAATCGGATTGATAAATTCCAAGTGGCTCGGGTTGTTCGCTAACGTAATGCGCGCTGATTTTGTTTCAGCGTCTTGAAGCTCCCGATTCGCCCCCAAATGGTATTTGACATCCCCCGTCCATCCGTAGCTGATTCCAATGGATCCCTCAGACGGTACAAGATCTTTGTTCGGCGCATGCTGGAATTCAGAGAAGATGATTTCATACGGTTTGCCGAGCACATGTGCCAAGACATTCA  >  CP006881/2062838‑2063109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑