Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,265,171	A→T	5.5%	D376E (GAT→GAA)	U712_11425 ←	Phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,265,171	0	A	T	5.5%	255.9 / 3.9	83	D376E (GAT→GAA)	U712_11425	Phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (35/43);  new base T (2/3);  total (37/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

ATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCGGATCATTG  >  CP006881/2265037‑2265306                                                                                                                                       |                                                                                                                                        aTCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTTTCAg                                                                                                                                     >  7:249366/1‑139 (MQ=255) aTCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAg                                                                                                                                     >  8:311316/1‑139 (MQ=255)           aTTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCTGTTTATTTGTTTGGAAATTATCAGCGATCGTTgc                                                                                                                           >  8:5689/1‑139 (MQ=255)            ttCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGAACGTTgcg                                                                                                                          >  5:331590/1‑139 (MQ=255)             tCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTgcgc                                                                                                                         >  5:205538/1‑139 (MQ=255)                        ttGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTTTCAGCGATCGTTGCGCCAATATCGGc                                                                                                               <  8:249366/138‑1 (MQ=255)                         tGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCa                                                                                                              <  3:203391/138‑1 (MQ=255)                             taataaCAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATg                                                                                                          <  7:56975/138‑1 (MQ=255)                                taaAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATc                                                                                                      >  3:202448/1‑139 (MQ=255)                                taaAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATc                                                                                                      >  8:103866/1‑139 (MQ=255)                                     gCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGc                                                                                                   >  8:339904/1‑137 (MQ=255)                                            cgcgTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGgc                                                                                            <  8:273883/137‑1 (MQ=255)                                            cgcgTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTAATGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGgcag                                                                                          >  7:349569/1‑139 (MQ=255)                                            cgcgATCAATTCTGTCCTTCAAGCAACAGTACCCCTATTGTAATAAAGATAAAAAGCTTTTCCGGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGTTCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGgc                                                                                            <  1:58598/137‑1 (MQ=255)                                                 tCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCAttt                                                                                     >  5:158617/1‑139 (MQ=255)                                                   aaTTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTg                                                                                    <  4:9843/138‑1 (MQ=255)                                                    aTTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTAGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCTCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTgcg                                                                                  <  7:5689/139‑1 (MQ=255)                                                       cTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCt                                                                                <  8:49574/138‑1 (MQ=255)                                                          tCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCgg                                                                                             >  8:29135/1‑122 (MQ=255)                                                          tCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCgg                                                                                             <  7:29135/122‑1 (MQ=255)                                                          tCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCt                                                                            >  7:89622/1‑139 (MQ=255)                                                          tCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCt                                                                            >  6:275381/1‑139 (MQ=255)                                                          tCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCt                                                                            >  5:151304/1‑139 (MQ=255)                                                               cAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGt                                                                       >  7:341808/1‑139 (MQ=255)                                                               cAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGt                                                                       >  5:191752/1‑139 (MQ=255)                                                                         tCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTg                                                              >  6:248474/1‑138 (MQ=255)                                                                              cTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTAt                                                                         >  4:2919/1‑122 (MQ=255)                                                                              cTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTAt                                                                         <  3:2919/122‑1 (MQ=255)                                                                              cTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTAGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCg                                                        <  5:132841/139‑1 (MQ=255)                                                                                   gTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   >  5:272207/1‑139 (MQ=255)                                                                                   gTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   >  4:181007/1‑139 (MQ=255)                                                                                   gTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   >  1:115810/1‑139 (MQ=255)                                                                                    taataaAGCTTCAAAGCTTTTCCAGTATTTCGGTTTAATTGTTTGGAAAATTTCATCCATCGTTCCGCCACTATCGGCAATTGTTTCTGCACCAGGCAGCATTTGCGCTTTCTTATGTTATTTGCTGTAAGCGAGAAt                                                   <  8:2978/138‑1 (MQ=255)                                                                                    taataaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   <  3:46808/138‑1 (MQ=255)                                                                                     attaaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGAATCTGCAAGCGGCAGCATTTGCGCTGTCTTATGTTTTTTGCTGTAAGCGAGCATCg                                                 >  2:75337/3‑139 (MQ=255)                                                                                      ataaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCgg                                                >  1:10551/1‑139 (MQ=255)                                                                                      ataaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCgg                                                >  5:258495/1‑139 (MQ=255)                                                                                      ataaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCg                                                 <  6:205538/138‑1 (MQ=255)                                                                                      ataaAGATAAAAAGCTTTTCCAGTAATTAAGTTTATATTTTTAGAAATTAACAGCGATCGTTGCGCCAATATCGTCAAATGTATATGCAAGCGGCAGCATTTTCGCTTTCTTATGTTTTTTGCTGTAAGCGATAATctg                                                >  6:136957/1‑137 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGTAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGc                                               <  5:275381/139‑1 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCCGCGATCGTTGCGCCAATAGCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGc                                               <  4:231209/139‑1 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCgg                                                >  1:103125/1‑138 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGc                                               <  6:151304/139‑1 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGc                                               <  3:181007/139‑1 (MQ=255)                                                                                       taaAGATAAAAAGCTTTTCCCGTATTTCGGTTTAGTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCgg                                                >  2:18022/1‑138 (MQ=255)                                                                                         aaGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGca                                              >  6:6786/1‑138 (MQ=255)                                                                                              aaaaaGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTTTCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCg                                                        <  8:163831/123‑1 (MQ=255)                                                                                              aaaaaGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTTTCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCg                                                        >  7:163831/1‑123 (MQ=255)                                                                                                aaaGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTcg                                      <  6:158617/139‑1 (MQ=255)                                                                                                aaaGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTcg                                      <  8:341808/139‑1 (MQ=255)                                                                                                aaaGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTGGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTcg                                      <  4:99582/139‑1 (MQ=255)                                                                                                   gCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgt                                   <  6:166566/139‑1 (MQ=255)                                                                                                      tttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTgcg                                                                                  >  2:151091/1‑89 (MQ=255)                                                                                                      tttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTgcg                                                                                  <  1:151091/89‑1 (MQ=255)                                                                                                      tttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgtgt                                 >  3:66440/1‑138 (MQ=255)                                                                                                      tttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgtgt                                 <  4:66440/138‑1 (MQ=255)                                                                                                       ttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgtgt                                 <  6:258495/137‑1 (MQ=255)                                                                                                       ttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgtgt                                 <  7:248528/137‑1 (MQ=255)                                                                                                       ttCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCgtgt                                 <  7:339904/137‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  2:115810/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  5:248474/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  7:219823/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  5:185211/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  5:136957/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTCTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  6:191752/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGAATCAGCAAGCGGCAGCATTTGCGCTATCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  8:80772/139‑1 (MQ=255)                                                                                                          ccGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGAACGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGt                            <  6:331590/139‑1 (MQ=255)                                                                                                              aTTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACt                                        <  8:16626/123‑1 (MQ=255)                                                                                                              aTTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACt                                        >  7:16626/1‑123 (MQ=255)                                                                                                                 tCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGttttt                                                                   >  4:182468/1‑93 (MQ=255)                                                                                                                 tCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGttttt                                                                   <  3:182468/93‑1 (MQ=255)                                                                                                                    gTTTATTTGTTTGGAAATTATCACCGAGCGTTGCGCCAATATCGGCAAATGTATCTGCACGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCatg                  <  2:158776/139‑1 (MQ=255)                                                                                                                     tttATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCatga                 <  2:10551/139‑1 (MQ=255)                                                                                                                       tATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCatgat                <  7:311316/138‑1 (MQ=255)                                                                                                                        aTTTGTTTGGAAATTATCAGCGATGGTTGCGCCAATATCTGCAAATGTATCTGCAAGCGGCAGCATTTGCGATTTCTTATGTTTTTTGCTGTAAGAGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCatgatg               <  6:257102/138‑1 (MQ=255)                                                                                                                          ttgtttgGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTCCAAGCGGCAGCATTTGCCCTTTCTTAATTTTTTTGCTGAAAGCGAGAATCGGCACATACTCCCGTGTATGGTCAGTCCAAAGATGAAt            >  7:232045/1‑139 (MQ=255)                                                                                                                             tttcGACATTATCAGCGAGCGTTCCGCCAATCTCGGCAAATGTATCTGCCCGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAGCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCg          <  4:104005/138‑1 (MQ=255)                                                                                                                             gttgGAAATTATCAGCGAGCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCg          <  8:349569/137‑1 (MQ=255)                                                                                                                               tgGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   <  8:308808/95‑1 (MQ=255)                                                                                                                               tgGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAAt                                                   >  7:308808/1‑95 (MQ=255)                                                                                                                               tgGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCGGAt       >  1:274427/1‑139 (MQ=255)                                                                                                                               tgGAAATTATCAGCGATCGTTGCGCCAATATAGGCAAATGTATCTGCAAGCGGCAGCATTAGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCGGa        >  5:257102/1‑138 (MQ=255)                                                                                                                                    aTTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCGGATCATTg  <  7:103866/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCTTTGGAGATTCCGGCAGGTTTTGTTTAATAAAAGCGGCTGCGCGTTCAATTCTGTCCTTCAAGAAACAGTCCCCCTATTGTAATAAAGATAAAAAGCTTTTCCCGTATTTCGGTTTATTTGTTTGGAAATTATCAGCGATCGTTGCGCCAATATCGGCAAATGTATCTGCAAGCGGCAGCATTTGCGCTTTCTTATGTTTTTTGCTGTAAGCGAGAATCGGCACATACTCGCGTGTATGGTCAGTCCCATGATGAATCGGATCATTG  >  CP006881/2265037‑2265306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑