Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,723,173 T→C 7.9% K64K (AAA→AAG)  U712_14040 ← Uncharacterized protein ysmA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,723,1730TC 7.9% 190.4 / 5.2 63K64K (AAA→AAG) U712_14040Uncharacterized protein ysmA
Reads supporting (aligned to +/- strand):  ref base T (36/22);  new base C (2/3);  total (38/25)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAGTGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGAAAAAAGA  >  CP006881/2723049‑2723310
                                                                                                                            |                                                                                                                                         
gaTAGTGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGAGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACtcat                                                                                                                             >  6:80017/1‑139 (MQ=255)
     tGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCatat                                                                                                                        >  4:97967/1‑139 (MQ=255)
     tGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCatat                                                                                                                        >  6:133569/1‑139 (MQ=255)
     tGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCatat                                                                                                                        >  3:124589/1‑139 (MQ=255)
     tGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCatat                                                                                                                        >  6:296807/1‑139 (MQ=255)
       cAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTc                                                                                                                      >  8:202089/1‑139 (MQ=255)
          ggTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGc                                                                                                                   >  2:63172/1‑139 (MQ=255)
               gagaTGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCtttt                                                                                                               >  1:141869/1‑138 (MQ=255)
               gagaTGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCtttt                                                                                                               >  2:122306/1‑138 (MQ=255)
                     aaCTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGCTTGTTTTCACTCATCATATTCAGCTTTTTAAAAt                                                                                                         >  7:178158/1‑138 (MQ=255)
                     aaCTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGCTTGTTTTCACTCATCATATTCAGCTTTTTAAAAt                                                                                                         >  7:173251/1‑138 (MQ=255)
                      aCTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTGTTCACTCATCATATTCAGCTTTTTAAAAt                                                                                                         >  3:338882/1‑137 (MQ=255)
                          ccGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGaaa                                                                                                   >  7:7795/1‑139 (MQ=255)
                             aCTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATcc                                                                                                >  5:283737/1‑139 (MQ=255)
                              cttcgcTTTTTTGCACATCGACCCGCAACTCTTCATAAGGCAATACTTGGCGCAAATACTCGCATTGCTGGATTGCCACGTCTGTCATGGTCTCTTTGTTATCACTCATGATATTCAGCTTTTTATAATAATAAATCCg                                                                                               <  5:97430/136‑1 (MQ=255)
                              cTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGCTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCg                                                                                               <  8:178158/139‑1 (MQ=255)
                              cTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGCTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCg                                                                                               <  8:173251/139‑1 (MQ=255)
                                 cgcTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCAc                                                                                            <  2:206736/139‑1 (MQ=255)
                                 cgcTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCAc                                                                                            >  4:198636/1‑139 (MQ=255)
                                 cgcTTGTTTTAACATAGCTGCTAAACTCTTCATAAGGAATTAATTGGCGCAAATAATCGAATTGCTTGGTGGCCACGAATTTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCAGCAc                                                                                            <  7:222890/139‑1 (MQ=255)
                                    ttGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCAcctc                                                                                         >  3:43534/1‑139 (MQ=255)
                                           cacaTAGATGCGCAACTCTTCATAAGGCATGACTTGGCGAAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCaaa                                                                                   <  6:283737/138‑1 (MQ=255)
                                                    gcgcAACTCTTCATAAGGCATTAGTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATaaa                                                                          <  1:291911/138‑1 (MQ=255)
                                                     cgcAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAg                                                                        >  6:133004/1‑139 (MQ=255)
                                                        aaCTCTTCATACGGCAGGACTGGGCGAAAATAATCGCATTGCTGGCTGGCCACGACTGTCATGGTCTGTTTCTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATAAAAAAgggg                                                                     <  5:79350/139‑1 (MQ=255)
                                                        aaCTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCAc                                                                                                                                 >  5:170024/1‑79 (MQ=255)
                                                        aaCTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCAc                                                                                                                                 <  6:170024/79‑1 (MQ=255)
                                                         aCTCTTCATAAGGCATGACTTGGCGCAAATAAACGCAATGCTGGCTTGCCACGACTGTCATGGTCTGCTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGt                                                                    <  4:334919/139‑1 (MQ=255)
                                                           tctTCATAAGGCATGACTTGGCGAAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTa                                                                  <  5:133004/139‑1 (MQ=255)
                                                            ctTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCaaa                                                                                   <  7:135769/121‑1 (MQ=255)
                                                            ctTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCaaa                                                                                   >  8:135769/1‑121 (MQ=255)
                                                               cATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     >  8:105030/1‑132 (MQ=255)
                                                               cATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     <  7:105030/132‑1 (MQ=255)
                                                                    ggCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCa                                                         >  5:103468/1‑139 (MQ=255)
                                                                    ggCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATAT‑AAAGGGGTTACATTATTCat                                                        <  1:63172/139‑1 (MQ=255)
                                                                     gCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        <  4:229380/139‑1 (MQ=255)
                                                                     gCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        <  3:97967/139‑1 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGt                                                                    >  5:290068/1‑125 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCatat                                                      >  6:138902/1‑139 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        <  1:2497/137‑1 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        <  1:7934/137‑1 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        >  2:2497/1‑137 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCat                                                        >  2:7934/1‑137 (MQ=255)
                                                                       aTGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTT‑AAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATg                                                     >  2:202038/1‑139 (MQ=255)
                                                                       aTGACTTGGCGAAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGt                                                                    <  6:290068/125‑1 (MQ=255)
                                                                         gACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     <  1:30419/122‑1 (MQ=255)
                                                                         gACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     >  8:19990/1‑122 (MQ=255)
                                                                         gACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     >  2:30419/1‑122 (MQ=255)
                                                                         gACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGc                                                    >  3:217715/1‑139 (MQ=255)
                                                                         gACTTGGCGAAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     <  7:19990/122‑1 (MQ=255)
                                                                              ggCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCaaa                                               >  7:177492/1‑139 (MQ=255)
                                                                                    aataatCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTAATATGCCCAAACAtgtc                                         >  6:270480/1‑139 (MQ=255)
                                                                                              ttGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCGCCTCCTCAAAATATATAAAAGGGGTTACATTATTCa                                                         <  3:171793/113‑1 (MQ=255)
                                                                                              ttGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCGCCTCCTCAAAATATATAAAAGGGGTTACATTATTCa                                                         >  4:171793/1‑113 (MQ=255)
                                                                                               tGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAACCATGTCTGTCTCGGaa                               >  2:324395/1‑138 (MQ=255)
                                                                                                 cTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGaaaa                             >  6:196920/1‑138 (MQ=255)
                                                                                                  tGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATc                           <  6:103468/139‑1 (MQ=255)
                                                                                                          cACGACTGTCATGGTCTGTGTGTTTTCACTCATCATATTCATCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGGTACAGTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGAt                   <  2:215377/139‑1 (MQ=255)
                                                                                                              aCTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACATCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATCTCTGTCTCGGAAAATCTTACGGATACCt               >  7:129262/1‑139 (MQ=255)
                                                                                                                  tCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     <  2:324232/81‑1 (MQ=255)
                                                                                                                  tCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAgggg                                                                     >  1:324232/1‑81 (MQ=255)
                                                                                                                       gTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGaaa       >  2:1744/1‑138 (MQ=255)
                                                                                                                       gTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGaaa       >  6:101836/1‑138 (MQ=255)
                                                                                                                          tgtttgttGTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGaaaa      >  7:20934/1‑136 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCa                                                         >  3:312230/1‑83 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCa                                                         <  4:312230/83‑1 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAATTCTTACGGATACCTTATTGAAaaaaga  >  5:1180/1‑138 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGAAaaaaga  >  2:46643/1‑138 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGAAaaaaga  >  4:299527/1‑138 (MQ=255)
                                                                                                                            tttgtttTCACTCATCATATTCAGCTTTTAACATGAAGAAAACCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCATACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTAAAataaga  >  2:5423/1‑138 (MQ=255)
                                                                                                                            |                                                                                                                                         
GATAGTGCAAGGTAAGAGATGAACTGCCGACTGCGCTTGTTTTCACATAGATGCGCAACTCTTCATAAGGCATGACTTGGCGCAAATAATCGCATTGCTGGCTTGCCACGACTGTCATGGTCTGTTTGTTTTCACTCATCATATTCAGCTTTTTAAAATAAGAAATCCGCACCTCCTCAAAATATATAAAAGGGGTTACATTATTCATATGCCCAAACATGTCTGTCTCGGAAAATCTTACGGATACCTTATTGAAAAAAGA  >  CP006881/2723049‑2723310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑