| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,963,937 | G→A | 11.5% | intergenic (‑124/+156) | U712_15285 ← / ← U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,963,937 | 0 | G | A | 11.5% | 125.3 / 7.1 | 46 | intergenic (‑124/+156) | U712_15285/U712_15290 | Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (31/8); new base A (3/2); total (34/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
AGCCCTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTT > CP006881/2963800‑2964066 | aGCCCTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATg < 2:125378/138‑1 (MQ=255) cccTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCa > 8:164089/1‑138 (MQ=255) tGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAAtt > 2:178035/1‑139 (MQ=255) atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATcgcg > 5:345999/1‑139 (MQ=255) tatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGt > 4:257469/1‑139 (MQ=255) ctcctcctcTTTGCCATCCATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTgg < 8:359162/138‑1 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGc > 1:80767/1‑139 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGc > 2:263821/1‑139 (MQ=255) ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCCCATGCAAAATTATCGAGTTTGGc > 7:358165/1‑139 (MQ=255) ctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGc < 3:257469/136‑1 (MQ=255) ctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCCCATGCAAAATTATCGCGTTTGGCCAt < 8:358165/139‑1 (MQ=255) ctctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCAtttt > 3:139226/1‑139 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCAttt > 1:333957/1‑133 (MQ=255) tGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCAttt < 2:333957/133‑1 (MQ=255) ccATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑g < 1:178035/139‑1 (MQ=255) ccATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑g < 2:80767/139‑1 (MQ=255) tCAATGCCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCt > 5:215763/1‑138 (MQ=255) aaTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGAt > 2:17516/1‑139 (MQ=255) tCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTa < 4:261426/138‑1 (MQ=255) ttGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATACAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATaa > 8:172963/1‑138 (MQ=255) ttttATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATaaaaa > 7:332415/1‑138 (MQ=255) tgttgtCAGTTCTTAGACATGTGACATTATATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATGGCGTTTGGGCATTTTTGTCAA‑GCTGATTTACATAAAAAAACAGGCTggg > 6:360955/1‑139 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTcag > 4:1510/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTcag > 4:172872/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTcag > 4:356181/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTcag > 8:343905/1‑138 (MQ=255) tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTcag > 1:85470/1‑138 (MQ=255) atttCTTAGACATGTGACATTAAATTAGCCAAAAGAATA‑TTTTATATACAAACTACAAAAAGTTTCCCTAATGCAAAATTATCGCGTTTGGCAATTTTTGTCAA‑GATGATTTACATAAAAAAACCTGCTCAGTcagcag > 8:80416/3‑139 (MQ=255) aGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCTGCTGGGTcagcag > 4:7656/1‑139 (MQ=255) ttCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGcc > 6:259048/1‑139 (MQ=255) aGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTc > 1:24976/1‑138 (MQ=255) aGACATGTGACAATAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATATTTGTCAA‑GCAGATTTACATAAAAAAACCGGATGGGTCAGCAGCCGCTc > 1:307458/1‑138 (MQ=255) tgtgACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGTCTGGGTCAGCAGCCGGTCCAAAc > 3:255967/1‑138 (MQ=255) gtgACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCaacc > 4:264866/1‑135 (MQ=255) tgACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGTTCAGCAGCCGGTCCaacc > 1:242365/1‑134 (MQ=255) aaTTAACCAAAAATATGTTTTTATTAAAACACCAAAAAAATTTACCCACATCAAAAATTATGGC‑TTTGGCCATTTTTGCAAACGTTTATTAAAAAAAAAAACCCGCCTGGGTCACCCGCCGGTCCAAACGAGAAGGGAt < 2:24976/139‑1 (MQ=255) tG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt > 2:360008/1‑139 (MQ=255) g‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtt > 3:56622/1‑139 (MQ=255) atttaCACAGGACAAAAATTTTCCCTCATGCAAAATTATCGCGTTTGGCAATTTTTCTAAA‑GCTTATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 5:161230/138‑1 (MQ=255) attaaCACAGCAAAAAAAGTTTCCCTCATCCAAAATTATCGCGTTGGGCCTTTTTTGACAA‑GATAATTAAAAAAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 1:17516/138‑1 (MQ=255) aataacacacAACAAAAAGTTCCCCTAATAAAAAATTAGCGCGTTGGGCTATTTTTGACAA‑GCTTATTAAAAAAAAAACACCGGCTGGGGCCGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc < 7:172963/134‑1 (MQ=255) gACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca > 7:160241/1‑138 (MQ=255) gACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGca > 4:146305/1‑138 (MQ=255) aaaGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAgtgt > 6:97930/1‑139 (MQ=255) gTTTCCCTCATGAAAAATTATCGGGTTGGGCCTTTTTTGTCAA‑GCTTATTTAAAAAAAAAACCCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAt < 7:78767/139‑1 (MQ=255) ttCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGCGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCtttt > 5:237081/1‑139 (MQ=255) ttCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAttt > 5:218056/1‑139 (MQ=255) | AGCCCTTGTCTCATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATG‑TTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAA‑GCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTT > CP006881/2963800‑2964066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |