| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,223,085 | A→C | 20.9% | S133R (AGC→CGC) | U712_16600 → | putative oxidoreductase yvrD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,223,085 | 0 | A | C | 20.9% | 92.4 / 6.1 | 64 | S133R (AGC→CGC) | U712_16600 | putative oxidoreductase yvrD |
| Reads supporting (aligned to +/- strand): ref base A (37/13); new base C (4/10); total (41/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACCTTGGTTTCTTCGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGAAATGACAAAAGGCACAAATGTG > CP006881/3222952‑3223219 | caacCTTGGTTTCTTCGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGATCGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGc > 2:147980/1‑139 (MQ=255) aacCTTGGTTTCTTCGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCGGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑CGCGGcc < 8:181028/139‑1 (MQ=255) aacCTGGGTTTCTTCGTGTAAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAACCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGcc < 1:51064/139‑1 (MQ=255) gTTTCTTCGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCAttt > 6:252967/1‑138 (MQ=255) ttcGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAata > 6:114494/1‑139 (MQ=255) ttcGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAAGCAATATTTAGAAGTGATCGTGATGAGCTCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAAGTGATGCCAAAAACAACCGCCCGCATTTaaat > 1:185330/1‑137 (MQ=255) gAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTTATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATAtt < 8:221441/138‑1 (MQ=255) aaGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATCTTTCactta > 7:31509/1‑134 (MQ=255) ttCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAg > 4:99378/1‑139 (MQ=255) ttCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAg > 5:349509/1‑139 (MQ=255) ttCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATCCTTGCCAAAAAC‑AGCGGCCGCAGTTTAAATATTGCAAGTGAAGCAg > 5:209288/1‑139 (MQ=255) cGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGc > 6:345044/1‑139 (MQ=255) cGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGc > 7:326937/1‑139 (MQ=255) cGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGc > 3:251692/1‑139 (MQ=255) cGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGc > 5:28543/1‑139 (MQ=255) cGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGc > 5:263692/1‑139 (MQ=255) tgtgACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCaaa > 1:331275/1‑139 (MQ=255) cAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCa > 6:346592/1‑125 (MQ=255) cAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCa < 5:346592/125‑1 (MQ=255) aaGAGTGCAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTGCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCAAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGcc < 7:152084/136‑1 (MQ=255) gcgTGCAAACAATTTTTCGAAGTCACCGTGAGGAGCGCCGTGCGGACAAGCCGCCACTTCCTACCAAAAATGCTTCCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga < 1:159278/137‑1 (MQ=255) gagTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga < 8:122366/139‑1 (MQ=255) gagTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga > 3:52923/1‑139 (MQ=255) gagTGGAATAAATTTTTCGAAGTGAACGTTATGTGCGCCGTTCGTACAAGCCGTCAATTCCTTCCAAAAATGGTTGCCAAAAAC‑AGCGGCCGCATTTTTAATATTTCAAGTGAATCAGGCGTCAAACCGCTGCCAACga < 8:4573/139‑1 (MQ=255) gagGGGAATAAATATTTCGAAGTCAACGGGATGAGCGCCGTTCGCACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga < 5:42436/139‑1 (MQ=255) agTGTATTCAATATTTCGATGTCAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTCCAAAAACC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga < 2:331275/138‑1 (MQ=255) agTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGAAAGTGAAGCAGGCGTCAAACCGCTGCCAAAgat > 7:4573/1‑139 (MQ=255) agTGGAAACAATATTTCGAAGTCAACGTCCTGCGCGCCGGTCGGACAAGCCGCCACTTCCTACAAAAATTGCTAGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACgat < 1:271704/139‑1 (MQ=255) gTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACgatg < 4:180919/139‑1 (MQ=255) tGGAATCAATATTTCGAAGTGAACTTGATGAGCGCCGTTGGCACAAGCCGGAACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACgatg < 4:289599/138‑1 (MQ=255) tGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTTCAAGTGAAGCAGGCGTCAACCCGCTGCCAACgatga > 4:65738/1‑139 (MQ=255) tGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACgatga > 3:37649/1‑139 (MQ=255) tGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTACAAAAAAGCTTGCCAAAAAC‑AGCGGCCGCTTTTTAAATATTTCAAGTTGAACAGGCGTAAAACCCCTTCCAAccgtgt > 3:929/1‑134 (MQ=255) gAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCACCgatgat > 1:130751/1‑138 (MQ=255) gAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGAtt > 4:79893/1‑139 (MQ=255) tCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCa < 5:114494/139‑1 (MQ=255) atatTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCAGTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGAATCCATAc > 4:56845/1‑139 (MQ=255) aGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGAc > 3:56243/1‑138 (MQ=255) aGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGAc > 1:4941/1‑138 (MQ=255) aaCGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTGCAGACGGAATGACaaaac > 2:238727/1‑138 (MQ=255) gatgaGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCg < 8:178567/100‑1 (MQ=255) gatgaGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCg > 7:178567/1‑100 (MQ=255) agccgcgcCGTGCGGACACGCCGCCACTTCATACCAAAAATGCTGGCCAAAACC‑CGCGGCCGCATTTTAAATATTGCACGTGAAGCAGGCGGCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCAc < 3:150722/135‑1 (MQ=255) gcgcCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGAt > 1:81719/1‑139 (MQ=255) cgcCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGAt > 2:101074/1‑138 (MQ=255) cgcCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTCAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGAt > 3:249566/1‑138 (MQ=255) ccccGTTCGCACAAGCCGTCACTTCATACAAAAAATGCTTCCAAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCAAACGATGATTCCATACTCAATGAAAAAAACGGCACTGATc < 3:99378/137‑1 (MQ=255) ccGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAACCC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCGATACTCAATGACAAAAACGGCACTGATCAg > 5:180448/1‑139 (MQ=255) ttCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAACC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCt > 8:171326/1‑139 (MQ=255) aGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGAACAGCCTTTCAagag > 1:265112/1‑139 (MQ=255) aGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAagag > 8:4556/1‑139 (MQ=255) aCTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGc > 1:217973/1‑139 (MQ=255) aCTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGc > 1:198761/1‑139 (MQ=255) aCTTCCTACCAAAAATGCTTCCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGc < 3:143221/139‑1 (MQ=255) ccttccAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga < 6:75053/80‑1 (MQ=255) ccttccAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACga > 5:75053/1‑80 (MQ=255) acttacAAAAATGCTTGCCAAAAAC‑CGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCCACCCTGATGCCATACTCAATGTCAAAAACGGCACTGATAAGCCTTTCAAGAGGAATTGCTGa > 6:207064/2‑138 (MQ=255) cttccAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGaaa > 2:13516/1‑139 (MQ=255) cttccAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGaaa > 7:332333/1‑139 (MQ=255) cttccAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGaaa > 3:123716/1‑139 (MQ=255) ccaaaaaaC‑CCCGGCCGAATTTTAAATATTCCATGTGACGCCGGCGACAACCCGCGGCCAACTATGATTCCATACTCAATGACAAACACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGAAATGACAAAAGGcac < 6:263692/134‑1 (MQ=255) ccAAAACC‑CTCGGCCTCATTTAAAATATAGCAAGTGACGCAGGGGTCAATCCGGTGCGAACGTTGATTCCATACTCAATGACACAAACTGCACGGATCAGACTTTCAAGAGGAATCGCTGAAATGACAAAAGGCACaa < 7:84807/138‑1 (MQ=255) acaC‑AGCGGCCGCATTTAAAATATTCCAAGTGACGCCGGCGTAAAACCGCGGCAAACGATGATTCCATACTCAATCACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGAAATGACAAAAGGCACAAAtgtg < 2:81719/137‑1 (MQ=255) aaaC‑CGCGGCCGCATTTTAAATATTGCAAGTGACGCAGGCGACAACCCGCTGCCAACGATGATTCCATACTCAATCACAAACACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGAAATGACAAAAGGCACAAAtgtg < 4:123716/139‑1 (MQ=255) | CAACCTTGGTTTCTTCGAGGTAAAAGACTTCGCCGATGTGACAGATGAAGAGTGGAATCAATATTTCGAAGTGAACGTGATGAGCGCCGTTCGGACAAGCCGTCACTTCCTTCCAAAAATGCTTGCCAAAAAC‑AGCGGCCGCATTTTAAATATTGCAAGTGAAGCAGGCGTCAAACCGCTGCCAACGATGATTCCATACTCAATGACAAAAACGGCACTGATCAGCCTTTCAAGAGGAATGGCTGAAATGACAAAAGGCACAAATGTG > CP006881/3222952‑3223219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |