Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,232,856 T→A 6.1% Q72L (CAG→CTG)  U712_16655 ← Putative efflux system protein yvrP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,232,8560TA 6.1% 229.4 / 2.2 74Q72L (CAG→CTG) U712_16655Putative efflux system protein yvrP
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base A (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTTCATCAGAATGATCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACGGCAACTTGGTTCATGTGAGTAAAAAAGTAAAAACCG  >  CP006881/3232735‑3232993
                                                                                                                         |                                                                                                                                         
cACTTCATCAGAATGATCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTc                                                                                                                           <  8:165601/138‑1 (MQ=255)
  cTTCATCAGAATGATCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAAc                                                                                                                        >  4:314484/1‑139 (MQ=255)
            aTGATCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGaa                                                                                                              <  3:109204/139‑1 (MQ=255)
                tCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACg                                                                                                          >  3:312740/1‑139 (MQ=255)
                tCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACg                                                                                                          >  1:55155/1‑139 (MQ=255)
                tCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCAGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACg                                                                                                          >  3:136552/1‑139 (MQ=255)
                tCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCAGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACg                                                                                                          >  3:131285/1‑139 (MQ=255)
                    cGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTg                                                                                                      >  4:185957/1‑139 (MQ=255)
                    cGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTg                                                                                                      >  2:219807/1‑139 (MQ=255)
                     gCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTGTCCAGAAAACGATTgg                                                                                                     >  3:77631/1‑139 (MQ=255)
                     gCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCATTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTAAGGTTCAACCTATCCAGAAAACGTTTgg                                                                                                     >  7:330410/1‑139 (MQ=255)
                       ccTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGcc                                                                                                   <  4:304219/139‑1 (MQ=255)
                          cATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGa                                                                                                >  6:353812/1‑139 (MQ=255)
                           aTACTCAAAAAGCTTATCGCCCTTTTTAACATGATCGCCTTCTTTGACGTATGATTTTTTAATGATAACATTTTCTGAATCTATGTATACTTTCTGATGTTTCTCAGGATCAACCTTTCCAGAAAACGTATGGCCTGAt                                                                                               >  2:200097/1‑139 (MQ=255)
                              cTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCt                                                                                            <  4:312740/139‑1 (MQ=255)
                              cTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCt                                                                                            <  1:219807/139‑1 (MQ=255)
                              cTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCt                                                                                            <  3:185957/139‑1 (MQ=255)
                                 aaaaaGCTTATCTCCCTTTTTACCCTGATCGACTTCTTTGACGTATGTTTTTTTAATGCTTCCTTTTTCTGAATCTATGTATAATTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGaa                                                                                         >  4:276862/1‑139 (MQ=255)
                                 aaaaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGaa                                                                                                              <  8:79100/118‑1 (MQ=255)
                                 aaaaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGaa                                                                                                              >  7:79100/1‑118 (MQ=255)
                                 aaaaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGaa                                                                                         >  3:97083/1‑139 (MQ=255)
                                 aaaaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGaa                                                                                         >  5:202214/1‑139 (MQ=255)
                                 aaaaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCAATCTATACTTTCTGCTGTTTCTCAGCTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGa                                                                                          >  8:47212/1‑138 (MQ=255)
                                 aaaaaGCTTATCGCCCGTTTTAACCTGATCGCCTTCTTTGCAGTATGTTTTTTTAATGCTGCCTGTTTCTGAATCTAGGTATACTTTCTGCTGGTTCTCAGGTGCAACCTTTCCAGCACTCGTTTGGCCTGATTCTGaa                                                                                         >  8:224575/1‑139 (MQ=255)
                                  aataGCTTATCGACCTTTTTAAACTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCATGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAt                                                                                        <  1:200097/136‑1 (MQ=255)
                                   aaaGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATCTTTATTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCAGCTGTTTCTCAGCAACACCCTTACCAGAAAACGTTTGCCATGATTCTGTAtc                                                                                       >  6:112713/1‑139 (MQ=255)
                                     aGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAtctc                                                                                     <  6:202214/139‑1 (MQ=255)
                                      gCTTATCGCCCTTTTTAACGTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGTAtctc                                                                                     <  5:112713/138‑1 (MQ=255)
                                       cTTATCGCCCTTTTTATCCTGATCGCCTTATTTGAAGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   <  8:330410/139‑1 (MQ=255)
                                       cTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   <  8:94230/139‑1 (MQ=255)
                                       cTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   <  3:131789/139‑1 (MQ=255)
                                         tATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 >  3:335680/1‑139 (MQ=255)
                                         tATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 >  5:37074/1‑139 (MQ=255)
                                         tATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 >  3:183920/1‑139 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTTTGTTTTTTTAATGCTGCCGTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAATCGTTTGGCCAGATTCTGAATCtcctcc                                                                                 <  3:260614/138‑1 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  3:314484/138‑1 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  5:353812/138‑1 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  2:208870/138‑1 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCAGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  4:136552/138‑1 (MQ=255)
                                          aTCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCAGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  4:131285/138‑1 (MQ=255)
                                           tCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCATAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   >  4:23541/1‑135 (MQ=255)
                                           tCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   <  3:23541/135‑1 (MQ=255)
                                           tCGCCCTTTTTAACCTGATCGCCCTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTtct                                                                                                                                  >  4:247325/1‑88 (MQ=255)
                                           tCGCCCTTTTTAACCTGATCGCCCTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTtct                                                                                                                                  <  3:247325/88‑1 (MQ=255)
                                                 tttttAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAtctc                                                                                     <  8:295500/127‑1 (MQ=255)
                                                 tttttAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAtctc                                                                                     >  7:295500/1‑127 (MQ=255)
                                                        ccTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGAc                                                                  <  6:37074/139‑1 (MQ=255)
                                                           gATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTACCACCTTTCCAGAAAACGTTTCGCCTGATTCTGATTCTCCTCCCATGTATACCGCGACCGa                                                               >  8:29872/1‑139 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGaa                                                                                                              >  7:58540/1‑91 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGaa                                                                                                              <  8:58540/91‑1 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 >  1:56912/1‑120 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  2:56912/120‑1 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGAc                                                                  <  3:146620/135‑1 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGAc                                                                  >  4:146620/1‑135 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGa                                                               <  3:156620/138‑1 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGa                                                               >  4:156620/1‑138 (MQ=255)
                                                            aTCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGAt                                                              >  1:101222/1‑139 (MQ=255)
                                                            aTCGCATTCTTTGACGTTTGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGAt                                                              <  2:103392/139‑1 (MQ=255)
                                                             tCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGAtt                                                             <  2:101222/139‑1 (MQ=255)
                                                                       tGACGTAAGTTTTTTTAATGGTGACTTTTTCTGACTCTATTTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCATAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATa                                                      <  8:263367/136‑1 (MQ=255)
                                                                                                              tGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAtctc                                                                                     <  7:323866/66‑1 (MQ=255)
                                                                                                              tGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAAtctc                                                                                     >  8:323866/1‑66 (MQ=255)
                                                                                                                     tttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGcc                                                                                                   >  4:207279/1‑45 (MQ=255)
                                                                                                                     tttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGcc                                                                                                   <  3:207279/45‑1 (MQ=255)
                                                                                                                     tttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 <  4:300974/63‑1 (MQ=255)
                                                                                                                     tttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCtcctcc                                                                                 >  3:300974/1‑63 (MQ=255)
                                                                                                                      ttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   <  4:198446/60‑1 (MQ=255)
                                                                                                                      ttCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATctcct                                                                                   >  3:198446/1‑60 (MQ=255)
                                                                                                                      ttCTGCTGTATCTCAGGTTCACCCTTTCCAGAAAACGTTTCGCCTGATTCTGAAAATCCTCCCATGTATACCGCCACCGATTCAACAAAGGGCTCCGATGATAAGGCAAATTGGTTCATGTAATTAAAAAACTAAAAAc    >  8:284066/1‑139 (MQ=255)
                                                                                                                       tCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATa                                                      <  5:268397/88‑1 (MQ=255)
                                                                                                                       tCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATa                                                      >  6:268397/1‑88 (MQ=255)
                                                                                                                       tCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACgg                                     >  2:55851/1‑105 (MQ=255)
                                                                                                                       tCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACgg                                     <  1:55851/105‑1 (MQ=255)
                                                                                                                       tCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACGGCAACTTGGTTCATGTTAGTAAAAAAGTAAAAAcc   >  1:161859/1‑139 (MQ=255)
                                                                                                                        ctgctgTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACGGCAACTTGGTTCATGTGAGTAAAAAAGTAAAAACCg  >  7:304012/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                         
CACTTCATCAGAATGATCCTCGCCCTCATACTCAAAAAGCTTATCGCCCTTTTTAACCTGATCGCCTTCTTTGACGTATGTTTTTTTAATGCTGCCTTTTTCTGAATCTATGTATACTTTCTGCTGTTTCTCAGGTTCAACCTTTCCAGAAAACGTTTGGCCTGATTCTGAATCTCCTCCCATGTATACCGCGACCGATTCAGCATAGGGCTCCGATGATACGGCAACTTGGTTCATGTGAGTAAAAAAGTAAAAACCG  >  CP006881/3232735‑3232993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑