Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,299,106	T→A	6.1%	I44F (ATC→TTC)	U712_16975 ←	Phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,299,106	0	T	A	6.1%	213.2 / 3.9	66	I44F (ATC→TTC)	U712_16975	Phosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base T (28/34);  new base A (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CTTCTTTGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTATTCATGCCT  >  CP006881/3298974‑3299239                                                                                                                                     |                                                                                                                                      cttcttTGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTgg                                                                                                                                 <  3:286487/139‑1 (MQ=255) cttcttTGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTgg                                                                                                                                 <  1:90647/139‑1 (MQ=255) cttcttTGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTgg                                                                                                                                 <  3:162101/139‑1 (MQ=255)       tGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGAATGTTGGAAgcg                                                                                                                            >  3:15263/1‑138 (MQ=255)       tGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGAATGTTGGAAgcg                                                                                                                            >  3:10556/1‑138 (MQ=255)           aaGCAGTTCGCCGAGACGTTCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCgcagca                                                                                                                       <  8:298423/139‑1 (MQ=255)            aGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCAc                                                                                                                      >  8:123181/1‑139 (MQ=255)             gCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACg                                                                                                                     <  6:115438/139‑1 (MQ=255)               aGTTCGCCGCTACGTGCAGCGACAGGAGTTAAAAGAAGCTCCTCAACCTCTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   <  2:186848/139‑1 (MQ=255)               aGTTCGCCGAGACGTGCTGCGACAGGAGTTAAACGTAGCTACTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   <  7:236380/139‑1 (MQ=255)                 ttCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATa                                                                                                                 <  5:339399/139‑1 (MQ=255)                 ttCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATa                                                                                                                 <  6:52510/139‑1 (MQ=255)                   cGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATAc                                                                                                                <  6:224046/138‑1 (MQ=255)                            gTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTc                                                                                                      <  6:137035/139‑1 (MQ=255)                                    aCAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTc                                                                                                      >  8:144529/1‑131 (MQ=255)                                    aCAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTc                                                                                                      <  7:144529/131‑1 (MQ=255)                                     cAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTc                                                                                             >  2:90292/1‑139 (MQ=255)                                         aGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCg                                                                                         <  1:125596/139‑1 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   <  4:100382/103‑1 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   >  3:100382/1‑103 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTg                                                                               >  7:62221/1‑139 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTg                                                                               >  7:313694/1‑139 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTg                                                                               >  6:358919/1‑139 (MQ=255)                                                   aGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTg                                                                               >  6:316610/1‑139 (MQ=255)                                                     ctcctcAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGAATGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGa                                                                             <  4:15263/139‑1 (MQ=255)                                                     ctcctcAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGAATGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGa                                                                             <  4:10556/139‑1 (MQ=255)                                                               aCTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCTTCTTTCATTGGAACGTTAAAGt                                                                   <  7:148156/139‑1 (MQ=255)                                                                cTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTg                                                                                                    >  4:206528/1‑105 (MQ=255)                                                                cTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTg                                                                                                    <  3:206528/105‑1 (MQ=255)                                                                cTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGt                                                                   >  6:242712/1‑138 (MQ=255)                                                                cTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGt                                                                   >  5:211764/1‑138 (MQ=255)                                                                 ttCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTc                                                                                                      <  3:157039/102‑1 (MQ=255)                                                                 ttCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTc                                                                                                      >  4:157039/1‑102 (MQ=255)                                                                  tCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCaa                                                                >  8:302959/1‑139 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGATGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  1:90292/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGAGTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  6:321385/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  7:329509/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  7:302959/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  7:213006/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  7:123181/137‑1 (MQ=255)                                                                         tCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCGTTCATTGGAACGTTAAAGTCAACTCgg                                                           >  5:296788/1‑137 (MQ=255)                                                                                     aGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACttt                                             >  4:120976/1‑139 (MQ=255)                                                                                          cTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGAAGAATACCACTTTGCCtt                                        >  4:226956/1‑139 (MQ=255)                                                                                           tctcAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCttt                                       >  8:44369/4‑139 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTTGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           >  8:12289/1‑119 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCgg                                                           <  7:12289/119‑1 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCa                                                         <  7:121715/121‑1 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCa                                                         >  8:121715/1‑121 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCttt                                       >  3:143192/1‑139 (MQ=255)                                                                                           tCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCttt                                       >  5:12951/1‑139 (MQ=255)                                                                                                   agGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATa                                                    >  8:146405/1‑118 (MQ=255)                                                                                                   agGACTTTTGCGCATTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATa                                                    <  7:146405/118‑1 (MQ=255)                                                                                                           tGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   <  4:6004/47‑1 (MQ=255)                                                                                                           tGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGa                                                                                                                   >  3:6004/1‑47 (MQ=255)                                                                                                               ccTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGagag                   <  5:316610/139‑1 (MQ=255)                                                                                                               ccTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGagag                   >  6:343658/1‑139 (MQ=255)                                                                                                                    gTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTTATGTCCACGCGGCAGAATTCAACTTTTCCTTTTACGTCGATGTCTTTGAGAGttttt              >  4:8922/1‑139 (MQ=255)                                                                                                                         cAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTAt          <  4:143192/138‑1 (MQ=255)                                                                                                                          aaGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTATTc        <  5:343658/139‑1 (MQ=255)                                                                                                                          aaGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTATTc        <  3:120976/139‑1 (MQ=255)                                                                                                                              tgtTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGa                                                                             >  8:248173/1‑66 (MQ=255)                                                                                                                              tgtTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGa                                                                             <  7:248173/66‑1 (MQ=255)                                                                                                                              tgtTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGAt                              <  7:338115/113‑1 (MQ=255)                                                                                                                              tgtTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGAt                              >  8:338115/1‑113 (MQ=255)                                                                                                                              tgtTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGAGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTATTCATg     >  7:74195/1‑138 (MQ=255)                                                                                                                                tttGATTGTTGGATGCGCAGCAAGGATACGTGTATCGTATGTTACTTCCCCGTCTTTCATTTGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACTTAGATGTCTTTGAGAGTTTTTTTATTCATGCCt  <  6:296788/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      CTTCTTTGCCAAGCAGTTCGCCGAGACGTGCAGCGACAGGAGTTAAACGAAGCTCCTCAACCACTTCGCCTTTCGGGCGGCCTAAGTGGCTCGCAAGAAGGACTTTTGCGCCTTGGTCTGCAAGGTGTTTGATTGTTGGAAGCGCAGCACGGATACGTGTATCGTCTGTTACTTCCCCGTCTTTCATTGGAACGTTAAAGTCAACGCGGCAGAATACAACTTTGCCTTTTACGTCGATGTCTTTGAGAGTTTTTTTATTCATGCCT  >  CP006881/3298974‑3299239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑