Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,309,155 T→G 18.3% intergenic (‑48/+354) U712_17015 ← / ← U712_17020 putative amino acid permease YvbW/putative glycosylase yvbX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,309,1550TG18.3% 78.2 / 2.5 59intergenic (‑48/+354)U712_17015/U712_17020putative amino acid permease YvbW/putative glycosylase yvbX
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base G (6/5);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCGCCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGAATATTCA  >  CP006881/3309022‑3309289
                                                                                                                                     |                                                                                                                                      
ccTGCGCCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCGTCGCGTTTTCAAAAACAAAAAAGCCCGCCGGCGcct                                                                                                                                   >  1:133200/1‑139 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTTTCGTTTTTCATCCTATTCTACATCTACTTCTCCTTTTAAAAAAAAAAAAAGACCTCCGGCGccgacca                                                                                                                               >  2:59266/1‑138 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTGCTGCTCTTCGACCTCTCCGTCTCGGTTTGAAAAACAAAAAAGCCCGCCGtctgcggcc                                                                                                                                >  5:266936/1‑132 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTGTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCGcctacc                                                                                                                                >  7:124246/1‑138 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAATCCCGCCGTCTcctacc                                                                                                                                >  7:219002/1‑138 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTcctacc                                                                                                                                <  1:22763/138‑1 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTcctacc                                                                                                                                <  1:2630/138‑1 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGGCTcctacc                                                                                                                                >  7:192892/1‑138 (MQ=255)
    cgcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGGTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTcctacc                                                                                                                                <  1:66167/138‑1 (MQ=255)
     gcCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTcctaccta                                                                                                                              <  8:219002/139‑1 (MQ=255)
       cGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACctaat                                                                                                                            <  5:41806/139‑1 (MQ=255)
         aTTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGtctc                                                                                                                                     <  6:175763/128‑1 (MQ=255)
         aTTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCGTCTCGTTTTCAAAAACAAAAAAGCCCGCCGtctc                                                                                                                                     >  5:175763/1‑128 (MQ=255)
           ggCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACctaatctaa                                                                                                                        <  7:77714/138‑1 (MQ=255)
            gCTCCGACAAGCGCCATCATCATAATATTGCGGGACGTCTTCGTGCTTTTTAACGTTTGATTGTCGTTTTTCTTCCTAATCTACCTCTACTTCTATATTTGAAAAACAAAAAAGCCCGCCGTCTCCTACctaatctta                                                                                                                        <  5:151861/138‑3 (MQ=255)
                       cgcCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCCCCGGCGCCCACCTAAACTAATAGATAAAaaga                                                                                                            >  4:299227/1‑139 (MQ=255)
                       cgcCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATACTCTTCTACCTCTCCTTAGCGTTTTCAAAAACAAAAAAGCCCGCCGTCGCCTACCTAAACTAATAGATAAagag                                                                                                             >  8:77714/1‑138 (MQ=255)
                           atcaaaataattTGGCGGGACGTAATGGTGCTTTTTAACGTTTTATTGTCTTTTTTCATCCTCTACTACCTCTCATTCTCTTTTAAAAAAAAAAAAACCCCCGCCGTCTCCTACCTAATATAATATATAAAGAcacgac                                                                                                        <  4:89446/133‑1 (MQ=255)
                           agcaacatAATTTGGCGGGCGGTCATCGTGCTTTTAAACGTTTGTTTGTGTTTTTTCAACCTCTACAACCTCCACTTCTCTTTTAAAAAAACAAAAACCCCCGCCGTCTCCTACCTAATATAATAGAAAAAGCgacgac                                                                                                        <  2:112275/134‑1 (MQ=255)
                           aacataatAATATGGGGGTACGGCATCGGGCGTTTTCAGGTTTGATTGTCTTTTTGAATCCTCTACTACCGCTCTTCCGCGTTTTCAAAAACAAAAACGCCCGCCGGCTCCTACCTATACTAATCGATAAAGCcacgac                                                                                                        <  6:177168/137‑1 (MQ=255)
                            tcatcatAATGTGGCGGGACGTCGTCGTGGGTTTTAACGTTTGATTGTGGTTTTTGCTCCGGTGGTACCGCGCCGTGGGGTTTTGAAAAACAAAAAAGCCCGCCGGCGGCGACCGAATCTAATAGTTAAAGTGacgccg                                                                                                       >  8:87572/1‑139 (MQ=255)
                            tcatcatAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTGAAAAACAAAAAAGCCCCCCGTCGCCGACCTAAAGGAATATATAAATAGacgacg                                                                                                       >  1:112275/1‑139 (MQ=255)
                            tcatcatAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATATAAAATATAAATAGacgacg                                                                                                       >  3:89446/1‑139 (MQ=255)
                            tcatcatAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTAAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAgacgac                                                                                                        >  7:48650/1‑138 (MQ=255)
                                       tGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCGCGTTTTCAAAAACAAAAACGCCCGCCGGCTCCTACCTAATCTAATAGATAACGACACGACGAGCCTTCGGCt                                                                                            <  5:147166/139‑1 (MQ=255)
                                          cGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAATCCCGCCGTCGACTACCTAATCTAATAGATAAAGAGACGCCGAGCCTTCGGCTgcc                                                                                         >  7:5756/1‑137 (MQ=255)
                                          cGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATATAATAGATAAAGAGACGACGAGCCTTCGGCTGAc                                                                                         >  6:254987/1‑139 (MQ=255)
                                          cGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCGCCTACCGAATCTAATAGATAAAGAGACGACCAGCCTTCGGccggt                                                                                         >  1:199875/1‑135 (MQ=255)
                                          cGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTAGGTTTCAAAAAAAAAAAAGCCCGCCGTCTCCGACCAAATCAAATAGATAAATAGAAGACGACCCTTAGGCTGAc                                                                                         >  7:287512/1‑139 (MQ=255)
                                            ggACGTCATCGTGCGTTTAAACTTTTGATTGGCGTTTTTCATCCTCTTAAACCTCTCATTCTCGTTTTCAAAAACAAAAACGCCCGCCGGCTCATACCAAATCAAAAATAAAAAGAGACGACGAGCCTTCGGCTGACcg                                                                                       <  7:305150/139‑1 (MQ=255)
                                             gACGTCATCGTGCGTTTTACCGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGCCGCGCCGTCGGCGGACcgc                                                                                      >  5:182572/1‑139 (MQ=255)
                                                    tCGTGCTTTTTAACGTTTGATTGGCGTTTTTCATCCTCTTATACCTCTCCTTCTCTTTTTAAAAAAAAAAAACCCCCGCCGTCTCCTACATAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCa                                                                               <  1:111571/139‑1 (MQ=255)
                                                      gTGCGTTTTAACGTTTGATTGTCGTGTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCAct                                                                             <  3:242973/139‑1 (MQ=255)
                                                            tttAACGTTTGATTGTCGTTTTTAATCCTCTTCTACCGCTCCTTCTCGGTTTAAAAAACAAAAAAGCCCGCCGGATCCAACCGAATCTAAAAGATAAATACACGACCAGCCTTCGGCGGACCGCGGGACCCCGCttgtt                                                                       >  2:111571/1‑139 (MQ=255)
                                                               acgtttttATTGCGGTTTTGCATCCTCTTCAACCTCCCCTTCTCTTTTAAAAAAAAAAAAACCCCCCCCGTCACAAACCTAATCAAATAGAAAAAGCCACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGAt                                                                    <  2:133200/135‑1 (MQ=255)
                                                                                tttCATCCTCTTCTACCTCTCCTTCTCGTTTTAAAAAAAAAAAAAGCCCGCCGTCTCCTACCTAATAAAATAGATAAAGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATcc                                                    <  1:31001/138‑1 (MQ=255)
                                                                                    agccTATTCAACCTCCCCTGCGCGTTTACAAAAACAAAACCCCCCGCCGTCCCCTCCCAAAACAAATAGAAAACGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCtt                                               <  6:85436/137‑1 (MQ=255)
                                                                                          atatACCGCTCCTTCGGGTTTACAAAAACAAAAAACCCCGCCGTCCCCTACCTAATCTAATAGATAAAGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGAcc                                         <  8:124246/136‑1 (MQ=255)
                                                                                            ctACCTCTCCTTCTCTTTTTCAAAAAAAAAAAAGCCCGCGGTCACCTACCTAATCTAATACATAAAGCGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGAc                                          <  6:146790/136‑1 (MQ=255)
                                                                                                  ctcCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACg                                  >  5:306742/1‑138 (MQ=255)
                                                                                                  ctcCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGAACTTTAACg                                  >  7:180301/1‑138 (MQ=255)
                                                                                                  ctcCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCGCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGCCCGCGGTTCCCCACTTGATTATTCTTCATGAAGAATCCAGCTTTTTACCTGTAACg                                  >  3:21851/1‑138 (MQ=255)
                                                                                                   tcCTTCTCGTTTAAAAAAACAAAAACGCCCGCCGGCTCCAACCTAATCAAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACg                                  <  8:5756/137‑1 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACcg                                                                                       <  5:123468/83‑1 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACcg                                                                                       >  6:123468/1‑83 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAg                               >  8:294672/1‑139 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTAATTAAGAATCCTGCTTTTGACCTGTTACGAAg                               >  1:259681/1‑139 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTATTCATGAAGAATACAGCTTTTGACCTGTAACGAAg                               >  3:138053/1‑139 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCGTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAg                               >  6:108788/1‑139 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCGCCTACCTAATCTAATAGAAAAAGAGACGACCAACCATCCGCTGACCCCGGTACCACTCTTTTTTATTCTTCATGAAGAAACCAGCGTATGACATGAAACCAAg                               >  3:129784/1‑139 (MQ=255)
                                                                                                    ccTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCACCTACCTAATCTAATAGATAAAGAGACCACGAGCCTTCGGCTGACCGCGGGACCACGGTTGTTGATTTTTCATGTATCATCCGGCTTTTGGACTGTAACGAAg                               >  6:229169/1‑139 (MQ=255)
                                                                                                        ctcGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGGACCCCTCTTGTTGATTATTCATGAAGAATCCTTCTTTTGACCGGTTATGAATGttt                           >  2:228169/1‑138 (MQ=255)
                                                                                                        cactttttAAAAAAAAAACACGCCCCCCGTCTCCTACCAAAAAAAATAGAAAAACAGACGACGAGCCTTCGGCTGACCGCGGAACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTa                           <  3:136124/135‑1 (MQ=255)
                                                                                                                caaaaacaaaacCGCCCGCCGGATCCTACCTAATATAATAGATAAAGCGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTa                    <  8:180301/138‑1 (MQ=255)
                                                                                                                aaaaaacaaaaacGCCCGCCGCCTCCTACCTAATATAATAGATAAAGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTaa                   <  6:287411/138‑1 (MQ=255)
                                                                                                                 aaaaacaaaaacGCCCGCCGTCTCCTACCTAATCTAATACATAACGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTaa                   <  8:19655/138‑1 (MQ=255)
                                                                                                                     acaaaaaCGCCCGCCGTCTCCTAACTAAAAAAAAACATAACGCCACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACAt              <  3:253750/139‑1 (MQ=255)
                                                                                                                         acaccccccccTTCTCCACCCTCACCAAATACAAAAAGCCACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGAATCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAggg          <  6:266936/134‑1 (MQ=255)
                                                                                                                          aaccccccccGACTCCAACCTAAACTAATACAAAACCCGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGa         <  3:299227/135‑1 (MQ=255)
                                                                                                                          aaaGCCCGCCGTCTCCAACCTAATATAATACATAAAGCGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGa         <  5:254987/139‑1 (MQ=255)
                                                                                                                               ccgccgTCTCCAAACTAATCTAATAGATAAAGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGAATAtt    <  2:259681/139‑1 (MQ=255)
                                                                                                                                 gcggTCACCTACATAATCAAATATAAAAAGACACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGAATATTCa  <  3:344741/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CCTGCGCCGATTGCTCCGCCCAGCGCCATCATCATAATATGGCGGGACGTCATCGTGCGTTTTAACGTTTGATTGTCGTTTTTCATCCTCTTCTACCTCTCCTTCTCGTTTTCAAAAACAAAAAAGCCCGCCGTCTCCTACCTAATCTAATAGATAAAGAGACGACGAGCCTTCGGCTGACCGCGGTACCACTCTTGTTGATTCTTCATGAAGAATCCTGCTTTTGACCTGTAACGAAGGTTAGCCGTTAAAACATAGGGAATATTCA  >  CP006881/3309022‑3309289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑