Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,801,996 G→A A327T (GCC→ACC)  U712_14440 → UPF0118 membrane protein ytvI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,801,9960GA100.0% 94.1 / NA 32A327T (GCC→ACC) U712_14440UPF0118 membrane protein ytvI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAAAA  >  CP006881/2801861‑2802130
                                                                                                                                       |                                                                                                                                      
ttATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTGCTTATTCAGCGGCACCTGACCCAGCCCCAGAAACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCAccc                                                                                                                                     >  3:216354/1‑139 (MQ=255)
            ggCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCt                                                                                                                         <  4:109226/139‑1 (MQ=255)
                 gCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTGCTTATTAAGCGGCAGCTGACAGAGCCCAAGAAACTAAGTAAAAATATCGGGATCGATCCGCAGGCAACCCTCATAACCCTGGTTGCCGGGGTTaaa                                                                                                                    >  8:268966/1‑139 (MQ=255)
                         aaGCAATCGGAATTGGTCTGCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            <  7:71831/139‑1 (MQ=255)
                         aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            <  1:197392/139‑1 (MQ=255)
                           gCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGCCAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATACGCTGGCTCCACTCATCACCCGGTTTGCCGGCTTTAAATTATTGGGGt                                                                                                          >  2:222280/1‑139 (MQ=255)
                                             cTGTACCTCGTCGTTATTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGTTCGATACGCTGGCAACACTCATCACCCTGTTTGCCGGGTTTAAATTATTCGGGTTTTTTTGTCTGATTGCgg                                                                                        >  6:113759/1‑139 (MQ=255)
                                                tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                                     >  1:204703/1‑139 (MQ=255)
                                                   cTCGTCGTTCTTCTTCAGCGGCAGCTGACAGATCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCTGGACCgg                                                                                  <  4:101747/139‑1 (MQ=255)
                                                     cgtcgtTCTTATTCAGCGGCAGCTGACAGAACCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCAGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt                                                                                >  1:80013/1‑139 (MQ=255)
                                                     cgtcgtTATTATTAAGCGGCAGCTGACAGAGCCCAACATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGGTTTTAGGACTGATTGCGGGACCGGcg                                                                                >  8:59969/1‑138 (MQ=255)
                                                              tATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGt                                                                        >  3:294893/1‑138 (MQ=255)
                                                               aTTCAGCGGCAGCTGACAGAACCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc                                                                       <  2:80013/138‑1 (MQ=255)
                                                                               cAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                                      <  4:294893/139‑1 (MQ=255)
                                                                                     ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            >  8:16368/1‑79 (MQ=255)
                                                                                     ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            <  7:16368/79‑1 (MQ=255)
                                                                                               aaGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCACGCTTTCATCACAACAGGCGCAtt                                       >  4:277107/1‑138 (MQ=255)
                                                                                               aaGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATAACAACAGGCGCAtt                                       >  4:50897/1‑138 (MQ=255)
                                                                                                aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTa                                      >  6:227311/1‑138 (MQ=255)
                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa                                 >  2:289414/1‑138 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGTGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                             <  7:268966/137‑1 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAatat                            <  1:143359/138‑1 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                             <  5:113759/137‑1 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                             <  8:118166/137‑1 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                             <  4:216354/137‑1 (MQ=255)
                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg                           >  4:53535/1‑139 (MQ=255)
                                                                                                            gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg                          <  2:204703/138‑1 (MQ=255)
                                                                                                               atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                                     >  5:92278/1‑76 (MQ=255)
                                                                                                               atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                                     <  6:92278/76‑1 (MQ=255)
                                                                                                                           gCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGt            <  1:289414/137‑1 (MQ=255)
                                                                                                                                    aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCaaaaa  >  2:109700/1‑138 (MQ=255)
                                                                                                                                    aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCaaaaa  >  4:140107/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAAAA  >  CP006881/2801861‑2802130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑