Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,095	T→A	intergenic (‑209/‑92)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,095	0	T	A	100.0%	265.4 / NA	73	intergenic (‑209/‑92)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/53);  total (20/53)

TACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑G‑TT‑TTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACG  >  CP006881/3722966‑3723223                                                                                                                                       |                                                                                                                                 tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGAc                                                                                                                           >  4:193470/1‑138 (MQ=255)        aTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTc                                                                                                                   >  2:1804/1‑139 (MQ=255)            tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                                                <  3:14145/138‑1 (MQ=255)                  ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACTAAAATTCGTaaaaatt                                                                                                          >  3:123168/1‑136 (MQ=255)                  ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTAGTaaaaatt                                                                                                          >  7:202924/1‑136 (MQ=255)                    tccTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                        <  6:29393/137‑2 (MQ=255)                    gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                        >  7:147265/1‑138 (MQ=255)                            tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                <  2:153044/138‑1 (MQ=255)                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  1:103652/138‑3 (MQ=255)                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  7:220556/138‑3 (MQ=255)                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  6:9908/138‑3 (MQ=255)                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  3:50891/138‑3 (MQ=255)                              ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  8:225495/138‑3 (MQ=255)                              ccAGTCACAATCACTCTTATTCAGATTAAGAATTCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                              <  3:33/138‑3 (MQ=255)                                  tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         <  2:287162/139‑1 (MQ=255)                                  tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         <  6:35137/139‑1 (MQ=255)                                  tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         <  2:129589/139‑1 (MQ=255)                                  ggacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         <  5:278844/137‑1 (MQ=255)                                    acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcac                                                                                        >  8:68607/1‑138 (MQ=255)                                       aTCCCGCTTATTCAGATTCAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  8:262214/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACTAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  4:123168/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  4:95147/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  4:296676/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  4:260677/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  4:225003/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  5:261084/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  6:75770/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  8:202924/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  8:69213/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  3:281535/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  3:269166/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  3:233187/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  8:284374/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  6:221343/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  1:147415/139‑1 (MQ=255)                                       aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  7:242722/139‑1 (MQ=255)                                       aTCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  8:126896/139‑1 (MQ=255)                                          ccGCTTATTCAGATTCAGAATACGCTTTCATCATAAATCAGGATAGCGGTTTAGTCAAC‑‑T‑C‑TTGTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  8:241526/138‑1 (MQ=255)                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  8:147265/139‑1 (MQ=255)                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  7:127313/138‑1 (MQ=255)                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  7:170227/138‑1 (MQ=255)                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  8:245594/138‑1 (MQ=255)                                          ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTCCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                  <  1:190473/138‑1 (MQ=255)                                            gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                               <  2:125864/139‑1 (MQ=255)                                                aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         <  1:237916/125‑1 (MQ=255)                                                aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                         >  2:237916/1‑125 (MQ=255)                                                aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                             <  3:219464/137‑1 (MQ=255)                                                 ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                                          <  1:100102/139‑1 (MQ=255)                                                      aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                     >  3:17478/1‑139 (MQ=255)                                                          agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    <  1:5045/120‑1 (MQ=255)                                                          agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                    >  2:5045/1‑120 (MQ=255)                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAtt                                                                                                                    >  7:110734/1‑82 (MQ=255)                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAtt                                                                                                                    <  8:110734/82‑1 (MQ=255)                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                               <  4:43271/119‑1 (MQ=255)                                                                cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                               >  3:43271/1‑119 (MQ=255)                                                                     tcatcaTAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTATTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaag                                                       >  2:94456/1‑138 (MQ=255)                                                                      catcatAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                                      >  8:172421/1‑138 (MQ=255)                                                                            aaaTCATGATAGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                                                >  1:137607/1‑138 (MQ=255)                                                                                      aGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAATATTCGTAAAAATTAATTGTGAATTACTTCACAATATCGTGGTATACTATTCTCAATCATGAAAGAAAGCAGGAAAAGACAAt                                     >  4:217767/1‑139 (MQ=255)                                                                                      aGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                               <  7:75840/97‑1 (MQ=255)                                                                                      aGCGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                               >  8:75840/1‑97 (MQ=255)                                                                                        cGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                    >  5:57910/1‑138 (MQ=255)                                                                                        cGTTTTCGTCAAC‑‑TAT‑TT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                    >  1:206379/1‑138 (MQ=255)                                                                                               gTCAAC‑‑T‑ATTT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTc                             <  3:104498/138‑1 (MQ=255)                                                                                               gTCAAC‑‑T‑ATTT‑TTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                            <  2:91306/139‑1 (MQ=255)                                                                                                     aactattt‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                          <  4:17478/132‑1 (MQ=255)                                                                                                     aactattt‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                          <  3:193470/132‑1 (MQ=255)                                                                                                      actattt‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                        <  7:68607/134‑1 (MQ=255)                                                                                                       ctattt‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAt                       >  1:166619/5‑139 (MQ=255)                                                                                                            t‑ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                   <  2:206379/138‑1 (MQ=255)                                                                                                               tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                 <  4:285146/138‑1 (MQ=255)                                                                                                                     tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTg           <  1:94456/138‑1 (MQ=255)                                                                                                                            tttGATATAACATGTAGAC‑AAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTatg  >  4:102177/1‑137 (MQ=255)                                                                                                                                       |                                                                                                                                 TACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑G‑TT‑TTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACG  >  CP006881/3722966‑3723223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑