Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	52,896	T→C	V45A (GTT→GCT)	U712_00225 →	Protein veg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	52,896	0	T	C	97.3%	261.0 / ‑1.9	75	V45A (GTT→GCT)	U712_00225	Protein veg
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (29/42);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGT  >  CP006881/52761‑53031                                                                                                                                        |                                                                                                                                        cAATGGCGAAGACGTTGTCCGATATTAAAAGATAGCTTGATGTGATTTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGcttt                                                                                                                                      <  4:157399/139‑1 (MQ=255)  aaTGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGctttt                                                                                                                                     <  4:288333/139‑1 (MQ=255)    tGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgt                                                                                                                                   >  3:145957/1‑139 (MQ=255)    tGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgt                                                                                                                                   >  3:185160/1‑139 (MQ=255)    tGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgt                                                                                                                                   >  6:127925/1‑139 (MQ=255)      gctaaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTCGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 <  6:77411/136‑1 (MQ=255)      gagAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAATGCAAACGGTGGCAGCCGAAAAACGATTGAGCGTGCGGGCATTTTAGCTGAGACGTACACTTCTGCTTTTGTGa                                                                                                                                 <  6:285626/137‑1 (MQ=255)      gCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 <  3:175168/139‑1 (MQ=255)      gCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 <  2:258887/139‑1 (MQ=255)      gCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 <  5:177860/139‑1 (MQ=255)      gCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 <  5:169675/139‑1 (MQ=255)       cGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGAt                                                                                                                                <  2:231125/139‑1 (MQ=255)         aaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATa                                                                                                                               >  1:172557/1‑138 (MQ=255)         aaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGAt                                                                                                                                <  6:165257/137‑1 (MQ=255)         aaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGAt                                                                                                                                >  5:165257/1‑137 (MQ=255)         aaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATa                                                                                                                               >  5:17832/1‑138 (MQ=255)         aaGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATa                                                                                                                               >  1:245676/1‑138 (MQ=255)            aCGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAAc                                                                                                                           <  2:172557/139‑1 (MQ=255)            aCGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAAc                                                                                                                           <  8:62338/139‑1 (MQ=255)                tGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCTGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAAc                                                                                                                           >  1:230661/1‑135 (MQ=255)                tGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAAc                                                                                                                           >  8:81024/1‑135 (MQ=255)                tGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATATTAGCTGACACGTACCCTTCTGCTATTGTCATACAAc                                                                                                                           >  1:171817/1‑135 (MQ=255)                ggTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAATGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGAATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAgc                                                                                                                       >  5:77411/2‑138 (MQ=255)                  tCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATc                                                                                                                     <  4:109552/139‑1 (MQ=255)                       tatTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGAc                                                                                                                <  7:40069/139‑1 (MQ=255)                       tatTAAAAGAGCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGAc                                                                                                                <  1:91334/139‑1 (MQ=255)                                 tCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGt                                                                                                      >  2:147693/1‑139 (MQ=255)                                   gCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGttt                                                                                                    >  5:256067/1‑139 (MQ=255)                                   gCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGttt                                                                                                    >  3:29660/1‑139 (MQ=255)                                    cTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAAc                                                                                                          >  6:158754/1‑132 (MQ=255)                                    cTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAAc                                                                                                          <  5:158754/132‑1 (MQ=255)                                    cTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCT‑‑GACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGa                                                                                                  >  5:130470/1‑138 (MQ=255)                                            gAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGtt                                                                                           <  6:17832/139‑1 (MQ=255)                                            gAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGtt                                                                                           <  2:245676/139‑1 (MQ=255)                                             aaTTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGttt                                                                                          >  6:258331/1‑139 (MQ=255)                                               tttAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCa                                                                                        <  4:145957/139‑1 (MQ=255)                                               tttAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCa                                                                                        <  5:258331/139‑1 (MQ=255)                                                 tAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCAt                                                                                       <  3:67016/138‑1 (MQ=255)                                                 tAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCAt                                                                                       <  7:126399/138‑1 (MQ=255)                                                  aGGTAAAAGTCTGACGTTAAAAGCAAACGGTGGCCTCCGAAAAACGATTGAGCGTTCTGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      <  6:153195/138‑1 (MQ=255)                                                  aGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      >  1:186257/1‑138 (MQ=255)                                                       aaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGaaa                                                                                                <  3:204120/123‑1 (MQ=255)                                                       aaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGaaa                                                                                                >  4:204120/1‑123 (MQ=255)                                                       aaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTa                                                                                <  7:81024/139‑1 (MQ=255)                                                             tGACGTTAAAAGCAATCGGGGGCCGGCGAAAATCGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGATTTTGTGATACATCTAGATCAAGACGAGAACTCGTTTGATAGAGTTTCATACAGTTATGCTGa                                                                          <  7:91405/139‑1 (MQ=255)                                                              gACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGat                                                                         >  3:84562/1‑139 (MQ=255)                                                               aCGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGa                                                                          <  8:28211/137‑1 (MQ=255)                                                                 gTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAtt                                                                      <  5:127925/139‑1 (MQ=255)                                                                      aaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGTGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAc                                                                 <  2:81212/139‑1 (MQ=255)                                                                      aaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAc                                                                 <  1:147693/139‑1 (MQ=255)                                                                            aCGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGAc                                                           >  1:15104/1‑139 (MQ=255)                                                                               gTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGt                                                        <  6:256067/139‑1 (MQ=255)                                                                                   ccgccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAg                                                    <  1:290926/139‑1 (MQ=255)                                                                                   ccgccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAg                                                    <  4:159805/139‑1 (MQ=255)                                                                                     gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  <  7:167703/139‑1 (MQ=255)                                                                                     gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTG‑AAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTg                                                 <  3:119374/139‑1 (MQ=255)                                                                                      ccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTg                                                 <  3:129918/139‑1 (MQ=255)                                                                                      ccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTg                                                 <  3:103357/139‑1 (MQ=255)                                                                                      ccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTg                                                 <  1:167502/139‑1 (MQ=255)                                                                                      ccgAAAAACGATTGAGCGTTCGGGCAATTTAGCCGAGACGTACCCTTCAGCTTTTGTGATACAACTCGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGCGGAGCTg                                                 <  2:196401/139‑1 (MQ=255)                                                                                           aaaCGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACAtt                                            <  1:75586/139‑1 (MQ=255)                                                                                                        ttCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTAATTGATGAcgcc                               >  3:116216/1‑139 (MQ=255)                                                                                                         tCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  >  2:288590/1‑119 (MQ=255)                                                                                                         tCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  <  1:288590/119‑1 (MQ=255)                                                                                                             ccATTTTAGCGGAGACGTACCCTTCGGCTTTTTGGATACAACTAGATCCAGACCAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTTATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAg                          <  8:272087/138‑1 (MQ=255)                                                                                                                ttttAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCt                        >  1:271961/1‑138 (MQ=255)                                                                                                                         agaCGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt              >  5:106478/1‑139 (MQ=255)                                                                                                                          gaCGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt              <  3:81268/138‑1 (MQ=255)                                                                                                                              tACCGTTCTGATTTTGTGATACAACTAGATCATGACGATAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGTTATTTTGTCTGAGACTGTTGAGCTGACATTTATTGATGACGCCGCAAGCTCAGTGGCATTTTAAc         <  3:116968/139‑1 (MQ=255)                                                                                                                              tACCCTTCTGCTTTTGGGAAACAACTAGATCACGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAAc         <  6:17611/139‑1 (MQ=255)                                                                                                                               aCCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACg        >  3:139439/1‑139 (MQ=255)                                                                                                                               aCCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACGGTTGAGCTGACATTTAATGATGACGCCGCACGATCAGTGGCATTTTAACg        >  3:199614/1‑139 (MQ=255)                                                                                                                                   ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTgaga                                                            >  1:141690/1‑83 (MQ=255)                                                                                                                                   ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTgaga                                                            <  2:141690/83‑1 (MQ=255)                                                                                                                                     cTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      <  6:284314/55‑1 (MQ=255)                                                                                                                                     cTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      >  5:284314/1‑55 (MQ=255)                                                                                                                                     cTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  7:82607/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        CAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGT  >  CP006881/52761‑53031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑