Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,162	C→T	intergenic (‑276/‑25)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,162	0	C	T	100.0%	205.6 / NA	57	intergenic (‑276/‑25)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/23);  total (34/23)

TCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑TTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGT  >  CP006881/3723034‑3723295                                                                                                                                   |                                                                                                                                      tcatcaTAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTTTATTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaag                                                                                                                               >  2:94456/1‑138 (MQ=255)  catcatAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                                                                                                              >  8:172421/1‑138 (MQ=255)        aaaTCATGATAGCGTTTTCGTCAACTAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                                                                                                                        >  1:137607/1‑138 (MQ=255)                  aGCGTTTTCGTCAACTAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAATATTCGTAAAAATTAATTGTGAATTACTTCACAATATCGTGGTATACTATTCTCAATCATGAAAGAAAGCAGGAAAAGACAAt                                                                                                             >  4:217767/1‑139 (MQ=255)                    cGTTTTCGTCAACTAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                                                                                            >  5:57910/1‑138 (MQ=255)                    cGTTTTCGTCAACTAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                                                                                            >  1:206379/1‑138 (MQ=255)                           gTCAACT‑ATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTc                                                                                                     <  3:104498/138‑1 (MQ=255)                           gTCAACT‑ATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                                                    <  2:91306/139‑1 (MQ=255)                               aactatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                                                                                                  <  4:17478/132‑1 (MQ=255)                               aactatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                                                                                                  <  3:193470/132‑1 (MQ=255)                                actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                                                                <  7:68607/134‑1 (MQ=255)                                 ctatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAt                                                                                               >  1:166619/5‑139 (MQ=255)                                      tttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                                                           <  2:206379/138‑1 (MQ=255)                                        tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                                                                         <  4:285146/138‑1 (MQ=255)                                              tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTg                                                                                   <  1:94456/138‑1 (MQ=255)                                                     tttGATATAACATGTAGAC‑AAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTatg                                                                          >  4:102177/1‑137 (MQ=255)                                                               catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTGTGaaa                                                                  >  6:292965/5‑137 (MQ=255)                                                                atgtAGAC‑AAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                                               <  3:102177/136‑1 (MQ=255)                                                                   tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                                                              >  7:124850/1‑138 (MQ=255)                                                                   tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                                                              >  1:115097/1‑138 (MQ=255)                                                                   tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAc                                                              >  3:35381/1‑138 (MQ=255)                                                                     gACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                                                            >  1:219895/1‑136 (MQ=255)                                                                     gACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGATCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACaaa                                                           <  2:32018/139‑1 (MQ=255)                                                                            aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                                                    <  4:46556/139‑1 (MQ=255)                                                                                                aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt                                <  1:1804/139‑1 (MQ=255)                                                                                                        cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt                        >  5:111464/1‑138 (MQ=255)                                                                                                        cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt                        >  4:214760/1‑138 (MQ=255)                                                                                                        cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt                        >  3:288322/1‑138 (MQ=255)                                                                                                        cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt                        >  8:29768/1‑138 (MQ=255)                                                                                                        cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAACCGTTATGTGCtt                        >  4:1942/1‑138 (MQ=255)                                                                                                            atatCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                    <  7:29768/139‑6 (MQ=255)                                                                                                            atatCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                    <  3:214760/139‑6 (MQ=255)                                                                                                            atatCGTGCTATACTAAGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                    <  1:85252/139‑6 (MQ=255)                                                                                                                cGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAt                >  2:22336/1‑139 (MQ=255)                                                                                                                cGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAt                >  4:29992/1‑139 (MQ=255)                                                                                                                  tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                                                                         >  5:135511/1‑64 (MQ=255)                                                                                                                  tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                                                           >  1:179119/1‑62 (MQ=255)                                                                                                                  tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                                                           <  2:179119/62‑1 (MQ=255)                                                                                                                       taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGcc         <  3:29992/139‑1 (MQ=255)                                                                                                                       taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGcc         <  1:22336/139‑1 (MQ=255)                                                                                                                       taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          >  8:77393/1‑138 (MQ=255)                                                                                                                       taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          >  3:83653/1‑138 (MQ=255)                                                                                                                       taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          <  2:219895/138‑1 (MQ=255)                                                                                                                       taCTAAGCTGAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGATGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          <  5:151499/138‑1 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATTCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          <  5:216381/133‑1 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGa                                                                    >  7:169614/1‑75 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGa                                                                    <  8:169614/75‑1 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc          >  6:216381/1‑133 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  4:65784/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  6:70842/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  7:11211/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  3:106858/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  8:15550/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  1:59961/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta     >  1:11649/1‑136 (MQ=255)                                                                                                                            tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgt      <  4:288322/137‑2 (MQ=255)                                                                                                                              cTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTAtat  >  8:62168/1‑137 (MQ=255)                                                                                                                                   |                                                                                                                                      TCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑TTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGT  >  CP006881/3723034‑3723295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑