Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,438 T→C T84T (ACT→ACC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,4380TC97.8% 163.1 / NA 46T84T (ACT→ACC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (15/30);  minor base A (1/0);  total (16/30)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGA  >  CP006881/3723306‑3723571
                                                                                                                                    |                                                                                                                                     
tggCATTACAGCCGCAAGCAAAAGAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                                                                                                                  <  3:217767/137‑1 (MQ=255)
tggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                                                                                                                  <  7:77393/137‑1 (MQ=255)
      tACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGACACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACAGGCAACCCcttt                                                                                                                           >  7:267889/1‑139 (MQ=255)
      tACAGACGCAAGCAAAACAGGCAGCTATTACTTTATGGCTGATTCTCCGTTTGCATTTCAGGCAGCCGCTGTCACTTTCGGGGCCGCTAATCTGATGATTGCTTACGGAGGAGTAGATTTATTTACCGGCAACACcttt                                                                                                                           >  2:96978/1‑139 (MQ=255)
         aGCCGGAAGGAAAAGAGGCAGCTAGTTCATTCTGGCTGAGTCTCCGTTTGCCTTTCCGCCAGCCTCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACTGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  5:18486/139‑1 (MQ=255)
         aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  8:158837/139‑1 (MQ=255)
         aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  2:11649/139‑1 (MQ=255)
         aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  3:4344/139‑1 (MQ=255)
         aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGGTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  5:178959/139‑1 (MQ=255)
         aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATGCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  5:292965/139‑1 (MQ=255)
              caagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAc                                                                                                                   <  1:46159/139‑1 (MQ=255)
                agcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAcc                                                                                                                  >  1:81769/1‑137 (MQ=255)
                                        aTGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAATGCATACGGAGGCTGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAta                                                                                          >  8:282270/1‑137 (MQ=255)
                                               aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCCCTCCGGAAAAAAATCACCTGGCg                                                                                  >  1:199121/1‑139 (MQ=255)
                                                  ctcCGTTTGCCTTTCCGTCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                <  4:206930/138‑3 (MQ=255)
                                                  ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTTTTTCACCTATACCGCGCTGCGGAAAAAAATCAGCTGTCgcg                                                                                <  2:4707/138‑3 (MQ=255)
                                                  ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                <  6:111464/138‑3 (MQ=255)
                                                     cgattGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                             <  1:277389/135‑1 (MQ=255)
                                                     cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                             <  6:228274/138‑1 (MQ=255)
                                                     cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                             <  4:226247/138‑1 (MQ=255)
                                                     cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                             <  4:2627/138‑1 (MQ=255)
                                                     cGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                               <  4:83653/136‑1 (MQ=255)
                                                           cctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCtat                                                                       <  5:189144/136‑3 (MQ=255)
                                                               tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTTTTATGATGATTGCTTACGGAGGCGGAGATTTATTTTCCGGCAACACCATTTATTTAACCAATACCCCGCGCCGGAAAAAAATAAGCAGGAGCGCCACCCTATACTtg                                                                  >  3:83211/1‑139 (MQ=255)
                                                               tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtg                                                                  >  7:246185/1‑139 (MQ=255)
                                                                 cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgtg                                                                <  4:75506/139‑1 (MQ=255)
                                                                                   tCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATTCAGCGATCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTTTgac                                                <  4:287509/137‑3 (MQ=255)
                                                                                   tCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCg                                               <  6:104715/138‑1 (MQ=255)
                                                                                           gCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAc                                                                                                                   <  1:93980/62‑1 (MQ=255)
                                                                                           gCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAc                                                                                                                   >  2:93980/1‑62 (MQ=255)
                                                                                                       aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        >  1:279629/1‑45 (MQ=255)
                                                                                                       aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                                                                                        <  2:279629/45‑1 (MQ=255)
                                                                                                       aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCg                                               <  2:151603/118‑1 (MQ=255)
                                                                                                       aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCg                                               >  1:151603/1‑118 (MQ=255)
                                                                                                         ttcTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCTCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCTGCGCCATTCtg                        >  1:58026/3‑138 (MQ=255)
                                                                                                         tGCTTACGGAGTCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                         <  2:58026/138‑1 (MQ=255)
                                                                                                         tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                               >  1:75400/1‑84 (MQ=255)
                                                                                                         tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                               <  2:75400/84‑1 (MQ=255)
                                                                                                         tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                >  6:138905/1‑81 (MQ=255)
                                                                                                         tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                <  5:138905/83‑3 (MQ=255)
                                                                                                         tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAttatt                        >  1:276784/1‑136 (MQ=255)
                                                                                                              aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCGGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGcc                                                >  6:292630/1‑110 (MQ=255)
                                                                                                              aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCGGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGcc                                                <  5:292630/110‑1 (MQ=255)
                                                                                                              aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                    <  6:161842/138‑1 (MQ=255)
                                                                                                              aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                    <  8:246185/138‑1 (MQ=255)
                                                                                                                               tataCCGGCAACACCTTTTATTTCACATATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGa  >  5:115874/3‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGA  >  CP006881/3723306‑3723571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑