Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,534	G→A	L116L (TTG→TTA)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,534	0	G	A	100.0%	225.0 / NA	65	L116L (TTG→TTA)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/22);  total (43/22)

TGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATG  >  CP006881/3723411‑3723669                                                                                                                            |                                                                                                                                        ttcTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCTCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCTGCGCCATTCtg                                                                                                                          >  1:58026/3‑138 (MQ=255) tGCTTACGGAGTCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                                                                                           <  2:58026/138‑1 (MQ=255) tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAttatt                                                                                                                          >  1:276784/1‑136 (MQ=255)      aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      <  8:246185/138‑1 (MQ=255)      aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      <  6:161842/138‑1 (MQ=255)                       tataCCGGCAACACCTTTTATTTCACATATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGa                                                                                                    >  5:115874/3‑139 (MQ=255)                              gCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACt                                                                                             >  2:5069/1‑139 (MQ=255)                              gCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACt                                                                                             >  3:259469/1‑139 (MQ=255)                              gCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACt                                                                                             >  7:286467/1‑139 (MQ=255)                                     cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCTGCAATTTAATCGGCGCCATTCTTTTTGCCATCCTTATCAGCGCGACGGTACTTTTTgag                                                                                      <  7:293372/138‑1 (MQ=255)                                     cttttATTTCACCTAAACCGCGATCGGTAAAAAAATCTGCGGGCGCTACACCCTATCCTTGTGGATGTCAAGCTATGCCGGCAATTTACTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                      <  2:276784/138‑1 (MQ=255)                                     cttttAATTCACCTATCCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                      <  7:282270/138‑1 (MQ=255)                                        ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTTGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                    <  6:115874/138‑1 (MQ=255)                                               acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      <  2:129598/94‑1 (MQ=255)                                               acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTg                                                                            >  2:60556/4‑139 (MQ=255)                                               acctataCCGCGCACCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      >  1:129598/4‑97 (MQ=255)                                                    taCCGCGCTCCGGTAAAAACTCAGCTGGCGCGACACCCTATACTTGTGGAGGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                        <  8:267889/138‑1 (MQ=255)                                                         cgcTCCGGAAAAAATTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGcc                                                                                                                    <  4:6452/89‑1 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGCCACACTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCGATTCTGTTTGCCATCCTGATGAGCGCGACGGGAGTTTTTGAGGAGCCTTCTGTTGATTCCtt                                                                  >  4:139241/1‑139 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGcc                                                                                                                    >  3:6452/1‑89 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGCTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCTTTCCtt                                                                  >  5:284303/1‑139 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                                                                                         <  3:31503/116‑1 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                                                                                         >  4:31503/1‑116 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                  >  8:93924/1‑139 (MQ=255)                                                         cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                  >  3:147137/1‑139 (MQ=255)                                                                         cagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                  <  4:259469/137‑1 (MQ=255)                                                                                                 gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTg                                                              <  8:65608/103‑1 (MQ=255)                                                                                                 gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTg                                                              <  4:261985/103‑1 (MQ=255)                                                                                                 gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTg                                                              >  7:65608/1‑103 (MQ=255)                                                                                                 gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTg                                                              >  3:261985/1‑103 (MQ=255)                                                                                                 gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGCGccgccggcg                          >  2:175461/1‑138 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      <  6:23207/46‑1 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      >  5:23207/1‑46 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCTACGGGACTTTTTGAGGAGCCTTCTGTTGATTCCTTTTTGATTAATTTGGCAGACCACAAAATGTAgcctccggagt                         >  4:158493/1‑134 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTGCTTTTTGATTCATTTTGCAGAGCAAAAAATGGAgccgccgggg                          >  3:114680/1‑134 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGCGCACAAAATGGAGCCGCCGGCtt                         >  3:209235/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  1:100563/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  8:219041/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  8:14164/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  4:273905/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCATTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  5:129144/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTACTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                         >  7:241974/1‑139 (MQ=255)                                                                                                  tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTGCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAATATGGAGccgccggcgt                         >  4:1815/1‑137 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      >  8:69252/1‑44 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                      <  7:69252/44‑1 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                            <  8:176622/102‑1 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                            >  7:176622/1‑102 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCCAAAAATGGAGCCGCCGGCTTcc                       >  4:47225/1‑139 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                       >  5:187332/1‑139 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                       >  4:150803/1‑139 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                       >  8:14000/1‑139 (MQ=255)                                                                                                    gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                       >  7:228202/1‑139 (MQ=255)                                                                                                      tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                      >  7:215211/1‑138 (MQ=255)                                                                                                         cAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaatt                  >  3:63590/1‑137 (MQ=255)                                                                                                          aaGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                            <  2:213200/96‑1 (MQ=255)                                                                                                          aaGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                            >  1:213200/1‑96 (MQ=255)                                                                                                             cTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttt              >  7:294814/1‑139 (MQ=255)                                                                                                                tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt            >  4:93730/1‑138 (MQ=255)                                                                                                                tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt            <  3:139987/138‑1 (MQ=255)                                                                                                                 gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt            >  4:223563/1‑137 (MQ=255)                                                                                                                     gCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCGTCCGAATTGTTTTTCAGAgg      >  5:237019/1‑139 (MQ=255)                                                                                                                      cAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAgg      >  6:284947/1‑138 (MQ=255)                                                                                                                      cAATTTAATCGGCGCCATTCGGTTTGCCATACTGATCCGCGCGACGGTACTTTTTGCTGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag       <  6:284303/137‑1 (MQ=255)                                                                                                                       aaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAggaa    <  3:93730/139‑3 (MQ=255)                                                                                                                         tttAATCGGCGCCATTCTTTTTGCCATCCTGAGCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAATGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg  <  2:199121/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                        TGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATG  >  CP006881/3723411‑3723669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑