Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,587	C→T	G80G (GGG→GGA)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,587	0	C	T	100.0%	181.8 / NA	60	G80G (GGG→GGA)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/33);  total (27/33)

TATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGG  >  CP006881/3724457‑3724713                                                                                                                                   |                                                                                                                               tataGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg                                                                                                                         <  4:220308/138‑1 (MQ=255)   taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       <  1:228538/138‑1 (MQ=255)   taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       <  4:26821/138‑1 (MQ=255)   taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       <  1:174701/138‑1 (MQ=255)   taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGAAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       <  8:53551/138‑1 (MQ=255)      agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                                                                   <  2:30591/139‑1 (MQ=255)      agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                                                                   >  5:242675/1‑139 (MQ=255)      agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAAAGc                                                                                                                   >  7:294425/1‑139 (MQ=255)      agCGCCTTTAACTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                                                                   <  3:12699/139‑1 (MQ=255)        cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCaaac                                                                                                                    >  5:30759/1‑133 (MQ=255)        cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGACGCGAGCTCTCCTTCCGCATAGCt                                                                                                                  >  1:32563/1‑138 (MQ=255)           ctttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                                                                              <  2:83021/138‑1 (MQ=255)              tATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                                                                            <  8:294425/138‑1 (MQ=255)                    aTGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCt                                                                                                       <  1:255785/137‑1 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCGTTTCGATGGCAAAGGACAAATGGTCGTAAAGCGCGATATGATTGTGATCGCTCAGCGGCGCCGCGAGCTCTCGTTCCGCATAGCTGATGATATCCTCAg                                                                                                    >  6:55382/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCTCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                                                                    >  6:129309/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                                                                    >  4:6356/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                                                                    >  5:18093/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                                                                    >  7:157117/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCAGCTGATATGCTCAg                                                                                                    >  6:37526/1‑139 (MQ=255)                     tGCAGCAATTTATTCTGCACAAGCAGCCAATTTTGAATCCTTTCGATCGAAAAGGAGAAATGGTCGGAAAGCGCGATATGAATCTGATCGCTCAGCGGCGCCTCGAGCTCTCCATCCGCATAGCATCTGATATCATCAg                                                                                                    >  8:80105/1‑139 (MQ=255)                       cagcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGc                                                                                                   <  6:18093/138‑1 (MQ=255)                       cagcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGc                                                                                                   <  4:69484/138‑1 (MQ=255)                        agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCTATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                                                                                 <  8:59137/139‑1 (MQ=255)                        agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                                                                                 <  6:242675/139‑1 (MQ=255)                        agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                                                                                 <  6:259099/139‑1 (MQ=255)                          caATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTAAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCGGATGATATCCTCAGCTAta                                                                                               >  8:9557/1‑138 (MQ=255)                                     gCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAACGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGATGATCTCCTCAGGTATTTCGATATGct                                                                                     >  6:178810/1‑138 (MQ=255)                                      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCTATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAg                                                                                                                    <  5:253617/106‑1 (MQ=255)                                      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGCTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                                                                      <  7:149562/136‑1 (MQ=255)                                      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAg                                                                                                                    >  6:253617/1‑106 (MQ=255)                                      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcc                                                                                   >  2:33302/1‑139 (MQ=255)                                      cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                                                                    <  4:248273/138‑1 (MQ=255)                                            cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                                                                             <  7:191922/139‑1 (MQ=255)                                            cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                                                                             <  3:80025/139‑1 (MQ=255)                                                   ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGttt                                                                      >  3:128038/1‑139 (MQ=255)                                                   ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCGTACCCATAGCTGTTGATTTCCTCCGCTATTTCTATATGGGCCTCCGGCAGGGttt                                                                      >  1:284657/1‑139 (MQ=255)                                                     ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg                                                                     >  6:240491/1‑138 (MQ=255)                                                         aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                >  8:41034/1‑139 (MQ=255)                                                              ttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgtg                                                                         <  8:233156/125‑1 (MQ=255)                                                              ttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgtg                                                                         >  7:233156/1‑125 (MQ=255)                                                                                     cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       <  8:249320/54‑1 (MQ=255)                                                                                     cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                                                       >  7:249320/3‑56 (MQ=255)                                                                                     cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcg                                         >  1:57817/3‑134 (MQ=255)                                                                                     cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcg                                         <  2:57817/132‑1 (MQ=255)                                                                                       gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                  <  7:41034/139‑1 (MQ=255)                                                                                              cgATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGAGCAAGACaaa                           >  6:198355/1‑139 (MQ=255)                                                                                               gATATTAATGGGATCGCTCTGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                          <  7:80105/139‑1 (MQ=255)                                                                                                       tgtgATCGCTCAGCGTCGCCGAGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCTCGACAAAGATCTTTTc                  <  8:210250/139‑1 (MQ=255)                                                                                                           aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              <  8:157117/139‑1 (MQ=255)                                                                                                           aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              <  5:177367/139‑1 (MQ=255)                                                                                                           aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              <  5:30341/139‑1 (MQ=255)                                                                                                           aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              >  7:144648/1‑139 (MQ=255)                                                                                                            gcGCTCAGCGGCTCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCTGCAGTGTTTGCAGGTTTTGTTTGAACTTTTGATTCTCGTCGCGCACGACATAGATCTTTTCctct              <  7:184799/137‑1 (MQ=255)                                                                                                               cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCCCTTTAt          >  4:127849/1‑139 (MQ=255)                                                                                                               cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              >  6:108157/1‑135 (MQ=255)                                                                                                               cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt              <  5:108157/135‑1 (MQ=255)                                                                                                                  aGCGGCGCCGCGAGCTCTCCTTCCGCATATCTGATGATATCCTCAGCTATTTCTATATGCTCCTCAGGCAGTGTTTGCAGGATTTGGTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa       <  8:195413/139‑1 (MQ=255)                                                                                                                    cggcgCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTTTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATa     <  7:154923/139‑1 (MQ=255)                                                                                                                       cgccgcGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTATATATTCTACTCCGGCAGTGTTTGAAGGATTTGTTTGAACTTTTAATTCTCGTCTCGCACGACAAAGATCTTTTCCACTTTTTTCATAtgg  >  4:112978/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                               TATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGG  >  CP006881/3724457‑3724713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑