| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 100.0% | 75.6 / NA | 27 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/13); total (14/13) | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 96.2% | 80.1 / NA | 27 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/11); minor base C (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT > CP006881/3725114‑3725369 || cccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc > 1:95199/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGaat > 3:292157/1‑137 (MQ=255) atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttct > 8:165188/1‑139 (MQ=255) cacaCCCGTAAATATACAAGAAAACGTTAAATACAAAAACACCCCTCCGTTTCTTTTGGGCCGGCAGC‑CTTTTTC‑GCAGCCGGTTTTCTCCGCCCCCGCCCGCAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAg < 2:95199/139‑1 (MQ=255) cGTAACTATACAACCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGC‑CTTTTTC‑GCCCCCCGTTTTCTCAGCCGCACCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc < 6:217877/139‑1 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc > 4:286810/1‑137 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 8:124825/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCACCCCGTTTTCTCAGCCGCATCCAGCAAGATAAGACGGCCGAGTTATTTTATAGACAACATACGGCTCTAGCaaaa > 6:191661/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCGTTTTGGCAGGCAGC‑CTTTTTA‑GCCGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCGTTTCTCGCAAAGTAGCGGCGCTAGCAAAAc > 2:50534/1‑139 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt > 8:87047/1‑138 (MQ=255) gCAACCGTTTCAAACAAAAACACCCCTCCGCTTCTTTTTGCCAGCCAGC‑CTTTTTC‑GCAGCCCGTTTACACAGCCGCAGCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 6:99270/139‑1 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCGCCGCTTCTTTTTGGCAGGCTGC‑CTTTTTA‑GCAGCCGGTTTTGTCCGCCGCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:40176/139‑1 (MQ=255) gCAAACGTTTCATACAAAAACAACCCTCCGCTTCTTTTTGGCCGCCAGC‑CTTTTTA‑GCCGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 1:44230/139‑1 (MQ=255) aaccttttCATACAAAAACATCCCGCCGCTTTTTTTTGGCTGGCCGCTT‑TTTTC‑GCCGCCCGTTTTCACACCCCCCCCCCCCACCAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 6:286813/134‑1 (MQ=255) ccttttAATAAAAAAACATCCCTCCGATTTTTTTTGGCCGGCCGC‑T‑TTTTACGCCGCCGTTTTTCCCCCCGGCCCCCCTCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:292157/136‑1 (MQ=255) acttttCAAACAAAAACACCCCACCGCTTCTTTTTGGCCGGCAGCAT‑TTTTA‑CCAGCCGGTTTTCACCGCCGCACCCCCAAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 1:220456/136‑1 (MQ=255) acttttAATAGAAAAACACCCCTCCGCTTCTTTTTGGCAGGCCGC‑CTTTTTA‑GCAGCCCTTTTTCTCAGCGCCAGCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 6:123105/136‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCAT‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 5:285168/139‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTACCGGCACTAGCAAAACAAATGAct > 8:40176/1‑139 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAATAGCGGCTCTATCAAAACTCAAGAc > 3:189558/1‑138 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCATTTTGGCAGGCAGC‑CTTTTTA‑GCACCCCGTTTTCTCAGCCGCAGCCCCCAAGAAAAGACCGCCGAATTCATTTCTCGCCAGCTAGAGGCTCATGCAAAACTCaacac > 5:4356/1‑134 (MQ=255) aCGTTTAATACAAAAACAGCCCACCGTTTCTTTTGGGCAGGCAGC‑CTTTTTC‑GCAGCCCTTTTTCTCAGCCCCCGCCCCCAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 6:118738/139‑1 (MQ=255) ttggacggcagC‑CTTTTTA‑GCCGCGGGTTTGCGCAGCCGCTGCACGCATCAAAAGCCGGCCGATTTCTTTTCTCTCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt < 1:6234/132‑1 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTAGTTTCTCGCAAACAAGCGGCTCAAGCAAAACGCATGACTGTATGAGAACCCCTGGAAAGAGAATACATTGTCAAAATATGATTGAAAAAAca > 2:205629/1‑137 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 4:144480/1‑139 (MQ=255) cAGCCGCCGCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAg < 7:190804/139‑1 (MQ=255) cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGCCCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGGTTTTAGAAGttt > 8:178891/1‑139 (MQ=255) || CCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT > CP006881/3725114‑3725369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |