BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,066:1	+A	93.4%	intergenic (‑180/‑121)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,066	1	.	A	93.4%	353.8 / 16.1	106	intergenic (‑180/‑121)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base . (5/2); new base A (39/60); total (44/62)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 106 total aligned reads displayed.

ACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTC‑AACT‑GT‑TTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGA  >  CP006881/3722934‑3723191

aCGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGTTCTTGCCTGCTATACCAGTCACAATACCGCTTATTCAGATTAACAATACTCTTTAAACATAAAACAAGATAGCGTTTACTTC‑AACT‑At‑tata                                                                                                                         >  8:186470/1‑136 (MQ=255)

 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACT‑At‑ttttt                                                                                                                        <  4:339647/139‑1 (MQ=255)

            cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAg                                                                                                              <  1:200977/139‑1 (MQ=255)

             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             >  5:17355/1‑139 (MQ=255)

             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  5:359365/139‑1 (MQ=255)

             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  1:209860/139‑1 (MQ=255)

             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  6:348470/139‑1 (MQ=255)

             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  8:333435/139‑1 (MQ=255)

             acaGAGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  2:169318/139‑1 (MQ=255)

              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGtt                                                                                                            <  7:69201/139‑1 (MQ=255)

              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGtt                                                                                                            <  8:180939/139‑1 (MQ=255)

               aGCGATCATTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCATAATCCCGCTTATTCAGATTAAGAATACGCTTTGATCATAAATCATGATAGCGTTTTGGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  7:186470/137‑1 (MQ=255)

                  gATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGAt                                                                                                        <  8:1862/139‑1 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             >  4:221705/1‑132 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  3:221705/132‑1 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATa                                                                                                       >  5:36378/1‑138 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATa                                                                                                       >  2:56326/1‑138 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATa                                                                                                       >  2:93064/1‑138 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATa                                                                                                       >  8:357999/1‑138 (MQ=255)

                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATa                                                                                                       >  6:25044/1‑138 (MQ=255)

                              tttACGCAAATTTTAGGTCTTGCCTGCTAAACCAGTCACAAACACGCTAATTCAGATAAAGAATACGCTTACATCATAAATCATGATAGAGTTTTCGTC‑AACT‑ATATTTTTATTTAAATAGTTTGATATAACATGTAGa                                                                                            >  6:120119/1‑139 (MQ=255)

                                tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGAc                                                                                           >  2:314404/1‑138 (MQ=255)

                                tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGAc                                                                                           >  5:324614/1‑138 (MQ=255)

                                 aCGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACa                                                                                          >  3:532/1‑138 (MQ=255)

                                 aCGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACa                                                                                          >  4:305723/1‑138 (MQ=255)

                                       aTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGt                                                                                                             <  4:262511/113‑1 (MQ=39)

                                           tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                <  5:35843/138‑1 (MQ=255)

                                               tCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaat                                                                           <  5:49673/139‑2 (MQ=255)

                                                  tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATata                                                                                                     >  1:265580/1‑108 (MQ=255)

                                                   gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTaaa                                                                                                                 >  8:295446/1‑97 (MQ=255)

                                                   gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTaaa                                                                                                                 <  7:295446/97‑1 (MQ=255)

                                                   gCCTGCTTTACCAGTCACAAT‑CCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAaattaatt                                                                      >  3:260063/1‑139 (MQ=255)

                                                        cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                   <  5:196027/138‑1 (MQ=255)

                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAGTTGTGaaa                                                                <  6:36378/138‑1 (MQ=255)

                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                <  6:17355/138‑1 (MQ=255)

                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                <  7:357999/138‑1 (MQ=255)

                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                <  1:93064/138‑1 (MQ=255)

                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                              <  3:210295/138‑3 (MQ=255)

                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                              <  3:302725/138‑3 (MQ=255)

                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                              <  4:358296/138‑3 (MQ=255)

                                                                 tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                         <  1:100256/139‑1 (MQ=255)

                                                                 tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                         <  8:59914/139‑1 (MQ=255)

                                                                 tCACAATCCCGCTTATTCAGATTAAGAATACGCTATCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                         <  3:248125/139‑1 (MQ=255)

                                                                  cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTTAAATACTTca                                                         >  6:208082/1‑138 (MQ=255)

                                                                  cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACT‑At‑ttttt                                                                                                                        >  4:141357/1‑74 (MQ=255)

                                                                  cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACT‑At‑ttttt                                                                                                                        <  3:141357/74‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  7:334749/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  8:332659/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  8:361331/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  1:155877/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  4:75550/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  3:145371/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  2:31394/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  3:305723/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  3:46916/139‑1 (MQ=255)

                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  2:180841/139‑1 (MQ=255)

                                                                       tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    >  4:46916/1‑138 (MQ=255)

                                                                       gatcGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  1:230832/135‑1 (MQ=255)

                                                                        cccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                  <  3:33845/139‑1 (MQ=255)

                                                                         ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                  <  1:314404/138‑1 (MQ=255)

                                                                         ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                  <  5:206544/138‑1 (MQ=255)

                                                                           gcatATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               <  3:260179/136‑1 (MQ=255)

                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               <  7:241473/139‑1 (MQ=255)

                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               <  5:120119/139‑1 (MQ=255)

                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               <  4:532/139‑1 (MQ=255)

                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               <  4:295511/138‑1 (MQ=255)

                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               >  3:295511/1‑138 (MQ=255)

                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                              >  3:130690/1‑139 (MQ=255)

                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                              >  6:224347/1‑139 (MQ=255)

                                                                             gtATTCAGATTAAGAATTCGCTTTCATCATATATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACTAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                             <  2:244993/138‑1 (MQ=255)

                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  5:7728/130‑1 (MQ=255)

                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    >  6:7728/1‑130 (MQ=255)

                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGGGAAATACTTCACAATATCGTGCt                                             <  5:345696/137‑1 (MQ=255)

                                                                                ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAATCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                          <  5:84967/139‑1 (MQ=255)

                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                         >  1:277582/1‑139 (MQ=255)

                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTAGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                         >  5:308231/1‑139 (MQ=255)

                                                                                   aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                        <  6:176843/138‑1 (MQ=255)

                                                                                   aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                        <  6:324614/138‑1 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCTTCATAATTCATGATAGCGTTTTCGTC‑AACT‑ATATTTTTAGTTTAATAGTTTGATATAAAATGTAGACAAAAATACTTAAAAATTAATTGTGAAATACTTACCAATATAGTGCTATTCTAt                                      >  3:201683/1‑138 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    >  2:293776/1‑124 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  1:293776/124‑1 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  1:61755/124‑1 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  5:97727/124‑1 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    >  2:61755/1‑124 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    >  6:97727/1‑124 (MQ=255)

                                                                                     aTTAAGAATACGCTTTCATCATAAATAATGATAGCGTTTTCTTCAAAAA‑TT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAATATTAAAAATTAATTGTGAAATACTTCAAAATATAGTCATATAcatg                                      >  2:230832/1‑135 (MQ=255)

                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                    <  6:85567/114‑1 (MQ=255)

                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                               >  7:7870/1‑119 (MQ=255)

                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                            >  6:362331/1‑138 (MQ=255)

                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                            >  7:81830/1‑138 (MQ=255)

                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                            >  5:302804/1‑138 (MQ=255)

                                                                                                  tttCATCATAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaa                        >  6:135376/1‑139 (MQ=255)

                                                                                                     catcatAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                        >  6:206742/1‑87 (MQ=255)

                                                                                                     catcatAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                        <  5:206742/88‑2 (MQ=255)

                                                                                                     catcatAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                      >  2:357703/1‑138 (MQ=255)

                                                                                                     catcatAAATCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAATTTGATATTACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTAAATCATGaaaga                      >  1:186407/1‑138 (MQ=255)

                                                                                                            aaTCATGATAGCGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGa              <  5:37150/139‑1 (MQ=255)

                                                                                                                       cGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg    >  3:19250/1‑138 (MQ=255)

                                                                                                                       cGTTTTCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg    >  5:210853/1‑138 (MQ=255)

                                                                                                                         ttttCGTC‑AACTATT‑TTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa  >  1:198697/1‑138 (MQ=255)

ACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTC‑AACT‑GT‑TTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGA  >  CP006881/3722934‑3723191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`