BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	744,068	A→T	9.5%	K502N (AAA→AAT)	U712_03585 →	Lipoprotein lipO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	744,068	0	A	T	9.5%	114.4 / 2.2	41	K502N (AAA→AAT)	U712_03585	Lipoprotein lipO
Reads supporting (aligned to +/- strand): ref base A (21/16); new base T (2/2); total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCATCCTCGACGCGAGAACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTGCGCT  >  CP006881/743930‑744199

catcatCCTCGACGCGAGAACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCaaaa                                                                                                                                     >  7:300172/1‑139 (MQ=255)

     tcCTCGACGCGAGAACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAAc                                                                                                                                >  6:366782/1‑139 (MQ=255)

          gACGCGAGAACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATg                                                                                                                           >  5:278421/1‑139 (MQ=255)

               gagaACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATCCgcct                                                                                                                      >  2:365325/1‑139 (MQ=255)

                agaACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCCGTGTAGATTTGGAAAAAAATCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCgcctg                                                                                                                     >  7:292927/1‑139 (MQ=255)

                         tttATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAATTAAACCGATGCGCCTGCCTTTcggc                                                                                                            >  8:2952/1‑139 (MQ=255)

                         tttATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAATTAAACCGATGCGCCTGCCTTTcggc                                                                                                            >  8:8337/1‑139 (MQ=255)

                                 tGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGccc                                                                                                    >  7:142510/1‑139 (MQ=255)

                                   gagaAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCg                                                                                                  >  7:289277/1‑139 (MQ=255)

                                   gagaAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCg                                                                                                  >  3:201736/1‑139 (MQ=255)

                                                    ggtctCGTTGGTGGGGGGGAGTTTTGCAAAAAATGCGGCGGCAATGAACTGATGATGGAATTTAACAAATTTTACAAATCTTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCACCGGTATCACGAAAGGAGaa                                                                                 <  6:124438/135‑1 (MQ=255)

                                                    gggTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAATTAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGaa                                                                                 <  7:2952/139‑1 (MQ=255)

                                                    gggTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGaa                                                                                 <  7:12061/139‑1 (MQ=255)

                                                     ggTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAATTAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGaa                                                                                 <  7:8337/138‑1 (MQ=255)

                                                      gTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACa                                                                               <  7:65291/139‑1 (MQ=255)

                                                                 cGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGaaa                                                                     <  7:2936/138‑1 (MQ=255)

                                                                              gAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGCACAGATATGGAAACAGTGCCgaag                                                          >  6:202034/1‑136 (MQ=255)

                                                                              caaaaaacGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCgaagaa                                                        <  5:366782/137‑1 (MQ=255)

                                                                                 aaaccgcggcgGCAATGAAGTGATCAAGGACTAGAAAAAATTTAACCAAACTAAAAAATAACCCGTTGCGCGGGCGTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAagaga                                                    <  1:365325/134‑1 (MQ=255)

                                                                                   cacgcggcgGCACTGAACTGATGAAGCACTTTAACAAATTTAACCAAGCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAgagaga                                                  <  1:192683/136‑1 (MQ=255)

                                                                                   aaaGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAgagaga                                                  <  8:300172/139‑1 (MQ=255)

                                                                                        ggcggcAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCAc                                             <  3:131247/139‑1 (MQ=255)

                                                                                                       atgaAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTg                              >  6:14289/1‑139 (MQ=255)

                                                                                                       atgaAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTg                              >  3:197929/1‑139 (MQ=255)

                                                                                                          aaGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGaa                           <  6:278421/139‑1 (MQ=255)

                                                                                                           aGGACTTGAACAAAATGTACCAATCGTCAAAAAAAACCTCTGCGCCGGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGaa                           <  2:177713/138‑1 (MQ=255)

                                                                                                            ggACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGaa                           >  8:207236/1‑137 (MQ=255)

                                                                                                            ggACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGaa                           >  7:370508/1‑137 (MQ=255)

                                                                                                            ggACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGaa                           >  3:310326/1‑137 (MQ=255)

                                                                                                               cTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTAACACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAgg                       >  8:327956/1‑138 (MQ=255)

                                                                                                               attGAACAAATTGTACCAATCGTAAAAATAAACGGATGCGCCGGCCTGTCGGGGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGc                      <  8:142510/138‑1 (MQ=255)

                                                                                                                   aaaaaaTTGTACAAATCGTAAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATc                   <  4:310326/135‑1 (MQ=255)

                                                                                                                   aaCAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATc                   <  8:370508/138‑1 (MQ=255)

                                                                                                                    aCAAATTGCACCAACCGACAAAATAACCCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAg                 <  5:242868/139‑1 (MQ=255)

                                                                                                                        aTTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGaa                                                                                 >  1:258262/1‑71 (MQ=255)

                                                                                                                        aTTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGaa                                                                                 <  2:258262/71‑1 (MQ=255)

                                                                                                                             aCCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGc        >  2:121044/1‑139 (MQ=255)

                                                                                                                             aCCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGc        >  3:257468/1‑139 (MQ=255)

                                                                                                                                 aTCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTgcg    >  2:10644/1‑139 (MQ=255)

                                                                                                                                 aTCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTgcg    >  7:142106/1‑139 (MQ=255)

                                                                                                                                 aTCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTgcg    >  5:288262/1‑139 (MQ=255)

                                                                                                                                    gTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTGCGCt  >  2:314203/1‑138 (MQ=255)

CATCATCCTCGACGCGAGAACACAATTTATCATTGGAGAAATTGATGAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGAAAGGAGAACAGATATGGAAACAGTGCCGAAGAAGAGAGATGCACCTGTTCTCACCGCTGGAAAAGGCATCAGCTGGATGGCTGCGCT  >  CP006881/743930‑744199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`