Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,725,082	A→C	100%	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	78.4 / NA	22	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/12);  total (10/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCA  >  CP006881/3724971‑3725213                                                                                                                |                                                                                                                                     cGCTTTCTGCTTTTCTACCTCATGCACCCCCACCCGCCATTCATAACCCGCGACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCgg                                                                                                            <  5:310524/138‑1 (MQ=255)  gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                                                          <  2:105994/139‑1 (MQ=255)                     cATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGAGCCGGATAGACCGCGGCCGCCGCAGTTTTTCTGTGCGGCGTCCCCCAGTTAATAaaaa                                                                                        >  2:195615/1‑138 (MQ=255)                              ccATCCGCCATTCATAAAACGTAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                              <  3:84700/139‑1 (MQ=255)                              ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                              <  8:372245/139‑1 (MQ=255)                              ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                              <  1:202076/139‑1 (MQ=255)                                      cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                      <  1:195615/136‑1 (MQ=255)                                           aTAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGTGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGa                                                                  <  1:94980/138‑1 (MQ=255)                                           aTAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAt                                                                 <  8:250670/139‑1 (MQ=255)                                           aTAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAt                                                                 <  7:208579/139‑1 (MQ=255)                                                                        cagccgccTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATcc                                    <  3:186280/137‑1 (MQ=255)                                                                           ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTAATCAAAAAGCACATCCACAACCGTAAAGATACAAGCAAACCTTTCATAGAAAAACATACCTc                                 >  7:69013/1‑139 (MQ=255)                                                                           ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                                                                     <  7:288755/71‑1 (MQ=255)                                                                           ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                                                                     >  8:288755/1‑71 (MQ=255)                                                                                         tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggc                   >  2:64467/1‑139 (MQ=255)                                                                                         tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTgg                    >  7:65109/1‑138 (MQ=255)                                                                                           tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcag                 >  3:276854/1‑139 (MQ=255)                                                                                             tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc               >  5:32318/1‑139 (MQ=255)                                                                                                   gAGCCGAATAGACCTCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCCCACCCGGAAAGAGACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCGTTTTGGCAGGCAGCC‑tt        >  5:190117/1‑139 (MQ=255)                                                                                                   gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGTAGTAACCCAGTTCATCAAAAAGACCATCCACACCCGTAAATATACAAGCAAAAGTTTCATAGAAAAACATCCCTCCGCTTATTTTTTgcaggaagacat         >  5:131682/1‑135 (MQ=255)                                                                                                         aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCATTTTag    >  7:97905/1‑138 (MQ=255)                                                                                                         aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑tttttagc  <  7:100833/139‑5 (MQ=255)                                                                                                                |                                                                                                                                     CGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCA  >  CP006881/3724971‑3725213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑