BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,729	T→G	6.1%	intergenic (+14/‑142)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,729	0	T	G	6.1%	11690.3 / 142.0	3891	intergenic (+14/‑142)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (303/3258); new base G (309/8); total (615/3276)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4076 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGATTCGC‑tgttt                                                                                                                                              >  4:134007/1‑139 (MQ=255)

     aCTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑gttttt                                                                                                                                             >  6:44659/1‑139 (MQ=255)

      atAAAAATCTGTTTAATAAAGAAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTa                                                                                                                                            <  2:180979/138‑1 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAAtttt                                                                                                                                      >  1:206791/1‑138 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                      >  5:88705/1‑139 (MQ=255)

             tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                     <  5:330501/139‑1 (MQ=255)

             tCAGTTTCATAAATCAATGATACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                     <  6:73098/139‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  1:100245/138‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAAATATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATATTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  8:164900/138‑1 (MQ=255)

                   tcatcaAGAAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGt                                                                                                                                <  8:191860/138‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                              <  5:70387/138‑1 (MQ=255)

                      tcaatcaaTGAAACACGCCAAACTAAAAAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa‑ca                                                                                                                           <  8:54970/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa‑ca                                                                                                                           <  6:15677/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa‑ca                                                                                                                           <  2:143413/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa‑ca                                                                                                                           <  1:17332/139‑1 (MQ=255)

                       caAGGAATGTAACACGCGAAATTAAACAATTTAAGGACGCTTACTTATGAGGAAGTATTGTCTATTTTTAATAGTTATGTATTTTTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGATAGTTa‑ca                                                                                                                           <  2:89543/138‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACg                                                                                                                         <  5:214690/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACg                                                                                                                         <  5:170422/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACg                                                                                                                         <  5:147866/139‑1 (MQ=255)

                         aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGt                                                                                                                        <  7:89231/139‑1 (MQ=255)

                           cAATGAATCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGtt                                                                                                                       <  1:286450/138‑1 (MQ=255)

                           cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTa                                                                                                                      <  1:76806/139‑1 (MQ=255)

                           cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTa                                                                                                                      <  3:212225/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGAATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACt                                                                                                                    <  7:232350/139‑1 (MQ=255)

                              tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTTTAAGTTa‑aacagtaat                                                                                                                    >  6:94338/1‑132 (MQ=255)

                               gAAACACGCCAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAAATTTTAAAGTTA‑AACCTTAATa                                                                                                                   >  8:310766/1‑138 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTCTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTaa                                                                                                                  <  6:253832/138‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTaaa                                                                                                                 <  1:125132/139‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTaa                                                                                                                  <  4:305737/138‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAACTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTaaa                                                                                                                 <  4:103486/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTGTTATTTA‑GAAGTTAATAAAtggta                                                                                                            >  7:281039/1‑137 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGaa                                                                                                            <  4:232717/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGaa                                                                                                            <  8:263069/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGaa                                                                                                            <  8:288413/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTA‑CACGTTACTAAAGGGaa                                                                                                            <  5:24603/139‑1 (MQ=255)

                                     agacAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGaa                                                                                                            <  3:342250/136‑1 (MQ=255)

                                         aaaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGga                                                                                                        <  2:354/139‑1 (MQ=255)

                                           attAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGA‑GTTTTTTAATATTGTAATTTT‑AACGTTAATAAAAGGAATTgag                                                                                                       >  7:209329/3‑138 (MQ=255)

                                           aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGgaga                                                                                                      <  3:165161/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTTTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  4:337425/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  6:182363/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  3:240523/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  7:116973/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  5:43544/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATa                                                                                                    <  3:116403/139‑1 (MQ=255)

                                                 cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAatt                                                                                                <  6:308607/139‑1 (MQ=255)

                                                  aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAatta                                                                                               <  6:218094/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:81608/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  2:310421/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:345316/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTA‑AAAGTTTATAAAGGGAATGGAGATTAattatt                                                                                             >  5:356332/1‑139 (MQ=255)

                                                      agacagccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  7:327132/133‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  3:314306/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAATTATTTTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  7:255041/139‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  8:112203/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  1:5715/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  1:14998/139‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  3:293465/138‑1 (MQ=255)

                                                                  attATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                               <  7:139684/138‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTAGTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                               <  7:303335/136‑1 (MQ=255)

                                                                      agAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                            <  2:5562/137‑1 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAAATTT‑CAAGTTTATAAAAGGAATGTAGATAAATTTTTATATATAATACTTACacaa                                                                          >  4:271744/1‑136 (MQ=255)

                                                                          cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTTTTATTTT‑AACAGTAATAAAAGATATTGTGATAAATTTTTATATATAATACTAACaacgtc                                                                        >  4:67817/1‑133 (MQ=255)

                                                                           aaGTTTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  8:137112/139‑1 (MQ=255)

                                                                            aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTT‑CACGTTACTAAATGGAATTGTGGTTAATTATTATATTTTATAAAGAAAGCTTccc                                                                      >  1:30606/1‑137 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTATATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  6:358998/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  6:334710/139‑1 (MQ=255)

                                                                              tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  3:13988/139‑1 (MQ=255)

                                                                               aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  8:229104/138‑1 (MQ=255)

                                                                                   tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTGTATGAGTCTC‑TTTTTTAATTTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAATAAGCTa                                                              <  1:342006/139‑1 (MQ=255)

                                                                                   tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAAGTAA‑ACCGTTA‑GAAATGGAATGGTGATTAATTTTTATATTTAATAAAGACCCCTTCCAAAAAGCt                                                               >  2:24851/1‑138 (MQ=255)

                                                                                   tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTT‑AAACTTTCTAAATGGATATGAGATAAATTATTTGATATAATACTGACAGCTTCCaagcatcg                                                               >  3:372476/1‑133 (MQ=255)

                                                                                     tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTAAAGGTT‑CACGTTTCGAAAGGGAATGGATATAAAATATTATATATACTAATGAAAGCTTACAAGAAGCTaa                                                             >  4:163652/1‑138 (MQ=255)

                                                                                       tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  7:164878/137‑1 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAATTTT‑AAAGTTACTAAAGGGAATTGTGATAAATTTTTATATTTAATAATTACAACTTCCAAGAAGCGAAaaag                                                         >  6:282992/1‑139 (MQ=255)

                                                                                         ttttATACTTATCTATTATTTAACGGGACGAAATAAATCTATGAGGCGC‑TTTTTTAAATTTGGAAAGGTA‑CACGTTACTAAACGGCATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  1:372772/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAATGTTAGTAATTAATTCTATGAGTGGA‑TTTTTTAAATTTGGAAAGTTA‑CACGTTAATAAAGTGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  4:189899/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  3:264766/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  4:97890/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  5:289234/138‑1 (MQ=255)

                                                                                                gTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTGGCAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTCTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                 <  5:192374/139‑1 (MQ=255)

                                                                                                 ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTATTTGGTAAGTTA‑AACGTTTCTAAATGGTAAGGTGATAAATTATTTTATTTAATAATGAACGCGTCCTATAAACGAAAGATGTcccgggc                                                >  4:367835/1‑135 (MQ=255)

                                                                                                   aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGGTAAGTTT‑CACGGTACTAAAGGGTAGGGAGTTAAATTTTTAGTTATAAGACGTACCGCTTGCAAGAAGCTAAAGCGGTAGCTAgcgc                                              >  8:7659/1‑139 (MQ=255)

                                                                                                        ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAATTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                           <  3:57437/137‑1 (MQ=255)

                                                                                                         tattTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GTAAGTTACCAGGTTA‑TAAATTGTATTGATATAAAATATTTTTTATACTAAATACCCCGACCAAGCATCTAAAGAGGTCCCGAACGccgcacg                                        >  8:333356/1‑134 (MQ=255)

                                                                                                          attTACCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCATCCAAGACGCTAAAGAGGTCCCTAGCGCCTACgg                                        <  7:368082/138‑1 (MQ=255)

                                                                                                             tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGGAAGTTA‑AACGTTTCAAAAAGGTATTGAGTTGAATTATTTGATTTTCTAAAGTCAGGTTACAAAAAGCTAAACAGGTCGCTTGCGCCGCCggggga                                    >  8:363204/1‑137 (MQ=255)

                                                                                                             tAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTTTTAATTTT‑CACCTTTCTAAAGGGAATGGTGGTAAATTTTTTGATTTAATAATGAAAGCAGACAAGAAGAGAAAAATGGCCCGAGCGCCAGAGGGGaa                                    >  2:104701/1‑139 (MQ=255)

                                                                                                              aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTT‑CACGTTACTAAAAGGAATGGAGATAAAATATTATATTTAATACTGACAGCGTCCAATAAGCGAAACAGGTCCCGAGCGCCGACCGGGCAt                                   >  6:131729/1‑139 (MQ=255)

                                                                                                               aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                   <  2:280835/138‑1 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                              <  3:193396‑M1/139‑3 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                              <  2:223633‑M1/139‑3 (MQ=255)

                                                                                                                            atTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                     <  6:10917‑M1/139‑12 (MQ=255)

                                                                                                                               cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  7:60956‑M1/138‑14 (MQ=255)

                                                                                                                               ataTGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTTCCCGCTACCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  5:121992‑M1/137‑14 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  1:2371‑M1/139‑18 (MQ=255)

                                                                                                                                   gAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  1:261859‑M1/138‑18 (MQ=255)

                                                                                                                                   gAGTCGC‑TTTTTTAAATTTCGAAAGTTA‑CACTTTACTAAAGGGAATTGAGATAAATTATTATATATACTAATGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:269419‑M1/138‑18 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAACTTTGGAAAGTTA‑CACGTTACTTAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  7:189316‑M1/139‑20 (MQ=255)

                                                                                                                                      tCGC‑TTTTTTAATTTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAATATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag            <  5:309072‑M1/138‑21 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTA‑CACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`