BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,735	A→T	9.6%	intergenic (+20/‑136)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,735	0	A	T	9.6%	11606.1 / inf	4063	intergenic (+20/‑136)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (192/3440); new base T (413/18); total (605/3458)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4165 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

 tAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTa                                                                                                                                                 >  8:333271/1‑138 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                           <  6:137738/139‑1 (MQ=255)

       taaGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                          <  8:255813/137‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                          <  4:30658/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                         <  1:100245/138‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                         <  7:297829/138‑1 (MQ=255)

           tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGaatt                                                                                                                                      >  5:77142/1‑137 (MQ=255)

               atcaatcaATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                   <  3:349189/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                   <  3:62670/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                   <  1:96430/138‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  7:274712/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  3:252958/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  3:8605/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  8:181849/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  5:273962/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  5:22009/139‑1 (MQ=255)

                tcaAGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                 <  5:313568/139‑1 (MQ=255)

                  aaGCACTGAAACACGCCCAAGTCAACAATTTAAGTACCATTACTTATGAGCCAGTAATGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTATATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  8:308453/139‑1 (MQ=255)

                  aaGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  1:124402/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  1:202958/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  7:18550/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  1:231420/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  3:300853/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑Cg                                                                                                                              <  7:80364/139‑1 (MQ=255)

                     cAATTAAACACGCCAAAGTAAACAATATAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTa                                                                                                                           <  6:295881/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACt                                                                                                                         <  6:9022/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAAATTTGTAAATTTACA‑‑GTtaataa                                                                                                                       >  2:38498/1‑138 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTaaa                                                                                                                      <  1:300870/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTaaa                                                                                                                      <  7:340524/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGaa                                                                                                                 <  7:50883/139‑1 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAAt                                                                                                                <  2:326231/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGAt                                                                                                          <  6:348941/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGAt                                                                                                          <  3:322324/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATa                                                                                                         <  3:37541/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATa                                                                                                         <  5:193691/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATa                                                                                                         <  6:371501/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  2:131933/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  2:162635/139‑1 (MQ=255)

                                            aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTACA‑CGTTACTAAATGGAATTGAGATAAatt                                                                                                     >  6:165352/1‑138 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:362633/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:92834/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:254555/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:131114/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:228718/139‑1 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAAttatta                                                                                                 <  7:247785/138‑1 (MQ=255)

                                                 aattACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  4:106716/135‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  2:26463/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  4:69449/138‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  5:172658/139‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  5:126268/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  4:233248/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAAATTTTTAAA‑TTAAA‑CCTTAATAAATAGAATGGTGATTAATTATtatatata                                                                                           >  2:239642/1‑138 (MQ=255)

                                                        aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGAAATTTTAA‑CGTTTCTAAAGGGAATGGAGATTAATTATTTtatataga                                                                                         >  4:339231/1‑136 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATTCTACTGa                                                                                    <  1:149252/139‑1 (MQ=255)

                                                                tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGc                                                                                <  3:202923/139‑1 (MQ=255)

                                                                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTCA‑CGTTAGTAAAGGGATTTTATATAAATTATTTTATATAATACTGAAAGCt                                                                               >  7:205134/1‑139 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  8:123989/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  4:164324/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:332715/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  3:131345/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:102918/139‑1 (MQ=255)

                                                                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAATTTAA‑CGTTAATAAATGGAATTGAGATAAATTATTATATATAATAATGACAGcataca                                                                           >  8:216492/1‑133 (MQ=255)

                                                                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAAATTAAA‑CCTTAAGAAAAGGAATGGAGATAATTTATTTTATATAAAACAGAAACCTTCCa                                                                           >  4:369999/1‑138 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAA‑CGTTAATAAAGGGAATGGAGATAAATTATTTTATTTACTACTGACAGCttac                                                                            >  5:151433/1‑134 (MQ=255)

                                                                        tATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTATATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  8:285642/139‑1 (MQ=255)

                                                                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  7:321320/139‑1 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTAAATTTTGAAACTTAAA‑ACTTTATAAAAGGAATGGAGATAAATTATTTTATATTATTCTTACAGCTTGCAAGCAGGTaa                                                                  >  1:33080/1‑138 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                               <  7:158498/139‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGAAAGTTTCA‑CGTTTCTTAATGGTATGGAGATAAATTATTTTATATAATTCTGACAGCTTACAAGAAGCTAAagag                                                              >  3:152909/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGTAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  6:191029/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  2:291946/138‑1 (MQ=255)

                                                                                      aaTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTc                                                           <  5:48655/138‑1 (MQ=255)

                                                                                      aaTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTc                                                           <  5:292858/138‑1 (MQ=255)

                                                                                        tAGTTATATATTATTTAACGGGAGGAAATAATTCAATGAGTCGC‑TTTTTTAAATTTTGAAAGTTACA‑CGTAACTAAATGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGTCCCt                                                        <  8:154025/139‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAAGTTTAA‑CGTTTCTAAAGGGAATGGAGATAAATTTTTAGATATTCTAATGACAGCTTGCAAGAAGCTTAAGAGGTCCCTAgc                                                     >  6:324559/1‑139 (MQ=255)

                                                                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGGTAAGTTTCA‑CGGTACTAAAGGGTAGGGAGTTAAATTTTTAGTTATAAGACGTACCGCTTGCAAGAAGCTAAAGCGGTAGCTAgcgc                                                   >  8:7659/1‑139 (MQ=255)

                                                                                                    attTACCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGATACA‑CGTTCCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                             <  1:51513/138‑1 (MQ=255)

                                                                                                    attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                             <  7:113078/138‑1 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGGAAGTTAAA‑CGTTTCAAAAAGGTATTGAGTTGAATTATTTGATTTTCTAAAGTCAGGTTACAAAAAGCTAAACAGGTCGCTTGCGCCGCCggggga                                         >  8:363204/1‑137 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAAGTTTCA‑AGGTATTTAATGGGTTGGGGATAAATTATTTTATTTACTAAAGACCTCGTCGAAGAAGCTTAAGAGGGGCCGACCGCCGACggggta                                         >  8:53237/1‑137 (MQ=255)

                                                                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑GGGTTTTTATTTTTTAATTTTAA‑AGTTTATAAAGTGTATGGGGATAAATTATTTTATTTAATAAATAAACCGTCCAAGCACCGAAAAAGGTACCTAGCGCCGACGGGGGAt                                        >  4:193396/1‑139 (MQ=255)

                                                                                                         actggagtaATTAATTCTATGAGTCTC‑TATTTTAAATATGGAAAATTACA‑CTTTACTAATGGGAATGTAGATAAATTATTAGATATACTACTTACAGCTTCCAACAAGATAAAGAGGTCCCTAGCGCCTACGGGTAAt                                        <  6:26785/135‑1 (MQ=255)

                                                                                                             gaggaAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTGAA‑CGTTAGGTAAGGGTATGGAGATTAATTTTTTGATATACTACTGACAGCGTGCAAGAAGCTAAAGAGGTGCCGAGCGCCTACGGGGGATTTggg                                   >  4:276516/1‑137 (MQ=255)

                                                                                                             gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                   <  5:251044‑M1/139‑3 (MQ=255)

                                                                                                               ggaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTAAACCGTTA‑TAAATGGTATTGAGATAAATTATTATATATAATACTTAAAGCTTGCAAGAACCGAAACAGGTCCCAACCGCCGACGGGGGATTTggggt                                 >  6:67370/1‑135 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  5:362052‑M1/139‑8 (MQ=255)

                                                                                                                            ttcttcgc‑tTTTTTAAATTTGGAAACTTACA‑CGTTAATTAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  8:103436‑M1/135‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:69604‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:279910‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:280480‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:106091‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGA‑TTTTTTAAATTTGGAAAGTTACA‑CGTTAATAAAGGGAATGTATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:366653‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTAGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATTGAGATAAATTCTTAGATATAATACTGACAGCTTCCACGAAGCTAAAGAGGTCCCTAGCGACTACGGGGAATTTGgggtacattgaaaaagg                    <  3:178545‑M1/139‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:22933‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:227770‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGTGAATGGAGATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  6:113887‑M1/139‑20 (MQ=255)

                                                                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  4:136699‑M1/139‑20 (MQ=255)

                                                                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑AGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTTACAGCTTCCAAGAAGGTAAAGAGGTCCCTAGCGCCTACGGGTAATTTGgggtacatagaaaaaggaa                  <  2:20120‑M1/139‑20 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:169887‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:70636‑M1/137‑26 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACA‑CGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`