BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,649	A→C	7.8%	Y260S (TAC→TCC)	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,649	0	A	C	7.8%	8001.5 / 146.4	3149	Y260S (TAC→TCC)	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2081/754); new base C (10/297); total (2098/1051)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3243 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

   cAGTACCAATTCTGCGCTCGCCCCTTCCGGCTGGCTGGTTTATTGATGATAAATATGGAGACTGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGCGTCAAGATGTTAAGCCATCCCGTATCCTAATTATCTaact                                                                                                                                        >  4:1385/1‑136 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGGAGTTATCTACACGACggg                                                                                                                                  >  1:184033/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  8:229555/1‑137 (MQ=255)

                 cgccgggCCGTTCCGGCTGGCTGTTTTTTTGCTCATAATTCTGCTGCCGGTGCGGGGGGGGGGCGCGGTATCATTGCAGCACTGGGGCCAGTTGTTACGCCCCCCCTTATCTTCGTAATCAACACGACGGGGAGTCAgg                                                                                                                          <  2:217372/134‑1 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  3:371388/1‑139 (MQ=255)

                      ggCCCTTCCGTCTGGCTGGTTTATTGCTGATAAATCTGTATCCGGTTAGCGTGGGTCTCGCGGTATAATTGCAGCACTGTGGCCAGATGGTAAGCAATCCCGTATCGTATTTATCTACACTACGGGGAgtcagtcatc                                                                                                                      >  2:188739/1‑136 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  7:295191/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  4:220061/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  4:6898/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  5:338284/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  2:119459/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  6:306583/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACt                                                                                                                     >  3:69648/1‑139 (MQ=255)

                            tCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAt                                                                                                               >  3:28394/1‑139 (MQ=255)

                              gggcgggcgGGTTTATTGCTGATAAATCGGGCGCGGGTGAGCGGGGGGCGCGCGTTATCATTGCAGCCCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  8:118676/138‑1 (MQ=255)

                              cggcggGGTGGTTTATTGCTCATAAAGCGGGCGCCGGTGCGCGGGGGGCTCGCGTTATCATTCCCGCACGGGGGCCAGTTGGTACGCCCCCCCGTAGCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  6:79513/139‑1 (MQ=255)

                               ggctgggtGGTTTATTGCTTAAAAATCTGGAGCGGGTGAGCGTGGGTATGGGGGAATCTTTGCAGCACTGGGGCCTGATGGTACCCCCTCCTGTATCGATGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGaa                                                                                                            <  6:137852/139‑1 (MQ=255)

                                gtggggtggTTTTTTGTTTATAACTCGGGTGCGGGTGGGGGGGGGGCGCGGGGTATCATTGCCGCTCTGGGGCCTTTTGGTAAGCCCTCCCGTATCTTTTTTATAAACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                           <  6:210155/133‑1 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                           >  1:220313/1‑139 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                           >  4:306902/1‑139 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                          >  5:284016/1‑138 (MQ=255)

                                     ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  3:258421/1‑138 (MQ=255)

                                              tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                                                   >  1:84194/1‑133 (MQ=255)

                                              tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                                                   >  1:354136/1‑133 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  5:315970/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  3:128262/1‑137 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  7:365375/1‑137 (MQ=255)

                                                             aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCATCTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                                              >  5:238670/1‑139 (MQ=255)

                                                                gggggcgCGGGGGGCTCGCGGTACAATGGCAGCACGGGGGCCAGAGGGTACGCCCTCCCGTAGCGTAGTTAACTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  8:15087/133‑1 (MQ=255)

                                                                gggTGAGCGTGGGGCTCGGTTTATCATGGCAGCCGTGGGGCCTGTTGGTCAGCCCTCCCGTTTGTTCGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  6:121163/138‑1 (MQ=255)

                                                                cggggcgCGGGGGCCTCGGGTTATCATTGCAGCCCGGGGGCACGATGTAAAGCCCTCCCGTATCGTAGTAAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  2:4252/133‑1 (MQ=255)

                                                                      gggggggTCTCGTGGTATCATTCCCGCCCGGGGGCCTGATGGTACGCCCGCCGGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  7:174663/135‑1 (MQ=255)

                                                                      gggggggTCTCGGGTTATAATTGCAGCACGGGGGCCAGTTGTTAAGCCCCCCCTTTTCTTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  1:101393/135‑1 (MQ=255)

                                                                      gggggggTCTCGCGTTAAGATTGCCGCAGTGGGCCCAGTTGGTAAGCCCTCCCGTATCGTATTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  7:267776/135‑1 (MQ=255)

                                                                          ggggcgcgcgGTTTCATTGCCGCAGGGGGGCCTGTTGGTAAGCCCTCCCGTATGGTTGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGc                                                                 <  2:274815/133‑1 (MQ=255)

                                                                                  cgGTATCATTGCCCCCAGGGGGCCAGAGGGTAAGCCCTCCCGTAGCGAAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTaa                                                         <  2:78524/139‑1 (MQ=255)

                                                                                      aTCATTGCAGCACTGGGGCCAGATGGTAAGCACTCCCGTAACGAAGTTATCTACACGCCGGCCAGTCAGGCAACTATGGATGAACTAAATAGACAGATCTCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGt                                                     >  8:206558/1‑139 (MQ=255)

                                                                                       tatttGCAGCAGTGGGGCCAGATTGACAGCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     <  1:42271/135‑1 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     >  6:153932/1‑138 (MQ=255)

                                                                                           tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTACGCATTGGTAACTGTCAGAc                                                >  6:181603/1‑139 (MQ=255)

                                                                                            gcagcaCGGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                <  7:313843/138‑1 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:346142/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:191397/1‑139 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:174360/1‑139 (MQ=255)

                                                                                                 aCTGGGCCCAGATGGTACGCCCGCCCGAACCGTCGTTAGCTCCACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  5:177789/139‑1 (MQ=255)

                                                                                                   tGGGGCCTGTTGGAACGCCCGCCCGTATCGTCGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:148960/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGTTGGAAAGCCCTCCCGAATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  5:104285/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCTTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:194227/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  5:206822/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  4:369042/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  7:334956/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  2:301739/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  3:151433/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  1:327394/1‑139 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:58073/139‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  8:300183/1‑135 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:125173/137‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  1:159905/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  7:218474/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  6:334510/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  8:254518/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATGAAGCATTGGTAACTGTCAGACCAAGTGTACt                                      >  8:41175/1‑139 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTTTACTCata                                  >  6:53432/1‑138 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  5:131930/139‑1 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  >  1:287429/1‑138 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTAACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCaga                                  >  5:93571/1‑136 (MQ=255)

                                                                                                            aTGTTACGCCCGCCCGTATTGTTGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                <  6:358536/138‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                >  5:265447/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  3:223684/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  7:77493/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGCCCAAGATTACTCATATATAcctg                          >  3:107829/1‑136 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                     >  8:184008/1‑139 (MQ=255)

                                                                                                                       tCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           <  8:32477/84‑1 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCCACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  1:62065/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  2:62004/1‑118 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                       >  4:273322/1‑119 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  3:318264/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  5:77689/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  2:243766/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  4:165617/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATAGATACTTTAgattga                   >  1:130455/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAGAAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  8:275533/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAGCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  2:343449/1‑139 (MQ=255)

                                                                                                                         ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  7:9709/124‑1 (MQ=255)

                                                                                                                            tATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGAttt                >  7:148406/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  4:33424/1‑138 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACTTGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGACTCACTGATTAAGCATTGGTAATTGTCATACCAAGTTTAATCATATAAAATTTTGATTGATATAAAACt          >  6:258034/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGc                                                                               <  6:283091/70‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  4:244966/1‑107 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  7:105684/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  2:155584/1‑139 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  8:61293/1‑138 (MQ=255)

                                                                                                                                     tATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  8:80709/1‑105 (MQ=255)

                                                                                                                                     tATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAt      >  1:254518/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGa                                                                       <  4:108778/73‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                                <  8:34892/80‑1 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  5:51008/1‑104 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  5:363407/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  7:237979/1‑139 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  4:189771/1‑138 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`