BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,006	T→G	5.8%	V40G (GTT→GGT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,006	0	T	G	5.8%	12264.3 / 112.6	4953	V40G (GTT→GGT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (2219/2309); new base G (381/18); total (2616/2337)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4970 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

     acagaGGAAACAGCTATGACCATGATTACGCCTAGCTTGCATGCCTGCAGGTCGACTCTAGTGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                         <  8:247803/138‑1 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                         <  3:265344/138‑1 (MQ=255)

      cccaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGCTCCCCGGGTCCCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGTATGGGGAAACCCAGGCGGTTCCCa                                                                                                                                       >  1:64760/3‑139 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAAAGTCGTGACTGGGAAAACCCTGGCGTTACCCAACt                                                                                                                                    >  2:2240/1‑139 (MQ=255)

          ggAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCCGGCGTTACCCAACt                                                                                                                                    >  5:67035/1‑138 (MQ=255)

           gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGCCTGGGAAAACCCTGGCGTTCCCCAACtt                                                                                                                                   >  4:203622/1‑138 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                                <  2:118505/139‑1 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTCGCGTTACCCAACTTAATCg                                                                                                                              <  1:130477/139‑1 (MQ=255)

                  cTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCt                                                                                                                           <  7:69612/139‑1 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                        >  3:170564/1‑139 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         >  6:187434/1‑138 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         <  7:211342/138‑1 (MQ=255)

                     tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         >  7:251034/1‑138 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCACCTTAATAGCCTTgcag                                                                                                                      >  5:372972/1‑139 (MQ=255)

                          aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcagca                                                                                                                    >  1:200944/1‑138 (MQ=255)

                          aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGGGGCTGGGGCAACCCAGGCGTTTCCCCAAATAATAGCCCTggcgccc                                                                                                                   >  1:310991/1‑133 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAACCCAGGCGGTTCACCACGTTATCGCCTTtcagcccc                                                                                                                  >  5:14287/1‑135 (MQ=255)

                                 cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTAAAAGTCGGGCATGGGTAAACCCAGGGGGTGCCCCACTTAATAGCCGTGCCGCCCATccccc                                                                                                            >  3:234629/1‑139 (MQ=255)

                                 cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATccccc                                                                                                            >  8:207741/1‑139 (MQ=255)

                                 cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACACAACATAAAAGCCTTTCAGCACATccccc                                                                                                            >  2:269288/1‑139 (MQ=255)

                                  gCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCt                                                                                                           >  6:160465/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCGGGCGTTACCCAACTTAATCGCCTTGCAGCCCATACCCCATTCg                                                                                                       >  8:345752/1‑139 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAccc                                                                                                                                        >  5:87672/1‑106 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTGTTACAACGTCGTGTCTGGGAAAACCCAGGCGGTTCCCAGCTTAAACGCCGTGCAGCACAAACCCCGTTCg                                                                                                       >  3:108367/1‑139 (MQ=255)

                                           gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  2:154512/139‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  1:283979/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  7:331184/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCCTTTTACAACGTCGTGACTGGGAAAAACCTGGCGTTAACCAACTTAAACGCCTTGACTCACATCCCCCTTTCCCCAGC‑t                                                                                                >  8:32368/1‑139 (MQ=255)

                                                    cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTAACCAACTTAATCGCCGTGCCGCCCCTCCCCCTTTCGCCCGC‑GGGCggta                                                                                         >  8:153182/1‑135 (MQ=255)

                                                       gTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                              <  4:352550/99‑1 (MQ=255)

                                                          gACTGTTGAGGATCCCGGTGTACCGAGCTCGAATTCACTGGCGGGGGTTTTACAACGTCGTGACTGGGAAAACCCTGGGGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATTGGGaa                                                                                  <  4:237182/139‑1 (MQ=255)

                                                          gACTCTCTAGGATCCCCGGGTACCGAGCTCGAATTCACTGTCCGTCGTGTTACAACGTCGTGACTGGGAAAACCAGGGCGGTACCCAAATTACTCGACTTGCAGCAAATCCCCCTTTCGCCATC‑TGGGGTAATAGCTaa                                                                                  <  3:195325/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGCGCTCGACTTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGACAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  2:84383/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCGTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  4:72122/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  5:325040/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  1:342632/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  7:44945/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGaa                                                                                  <  6:332653/139‑1 (MQ=255)

                                                          gACTATAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGG‑TGGCGTAATAGCGac                                                                                  <  6:312357/139‑2 (MQ=255)

                                                           aCTCTAGCGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACGGGGAAAACCCTGGCGTTACCCAACTTACTCGCCTTGCAGCACATCCCCCTTTCGCCCGC‑TGGCGTAATAGCGAag                                                                                 <  4:294968/139‑1 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAccc                                                                                                                                        <  7:278033/85‑1 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGCag                                                                                 >  8:226437/1‑139 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATAGCCGTGCAGCACATCCCCCGTTCGCCCGC‑TGGCGTAATAGCg                                                                                    >  6:282150/1‑136 (MQ=255)

                                                                 gagGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑AGGCGTAATACCGAAGAGGccc                                                                           >  7:105225/1‑139 (MQ=255)

                                                                 gagGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGACGGGACAGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCGTGCACCACATCCCCCGTTCGCCAGC‑TGGCGGAATAGCGAAGAGGccc                                                                           >  5:299263/1‑139 (MQ=255)

                                                                  cagATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCg                                                                          <  5:119641/137‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTGGTGACTGGGAAAACCCTGGCGGTACCCCACGTTATCTCCTTGCAGCACAGCCCCCTTTCGCCAGC‑TGGCGTATTTTCGAAGAGGccccgccc                                                                      >  4:96028/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                              <  3:88965/84‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                         <  4:161725/89‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  <  7:130914/112‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  8:40366/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAACCCACTTAATAGCCTTGCAGCACATCCCCCATTCGCCAGC‑AGGCGGAATAGCGAATAGGCCCCCAcc                                                                      >  4:39944/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCCCAAACCCCGTTCCCCCCC‑AGGCCGAATAGCGAAGAGGCCCGCAcc                                                                      >  3:325092/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTTCCCAACTTTATAGCCTTGCAGCACCTCCCCCATTCGCCAGC‑TGGCGTAATAGCGAATAGGCCCCCAcc                                                                      >  2:29541/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTAACCAACCTAATAGCCAAGCAGCACAAACCCCATTCGCCCGC‑AGGCCTAATATCGAATAGGCCCGccac                                                                      >  6:217843/1‑136 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGCCTGGGAAAACCCTGGCGTTACCCCACTTAATCCCCTTGCAGCACAGCCCCCGTTCGCCCGC‑TGGCCGAATAGCCAACAGGCCCGcccc                                                                      >  3:368773/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACATCGTTTCAGGGAAAACCCAGGCGTTACCAAAATTAATCCCCATGAACAACATACCCCGTTGGCCACC‑AGGCCTAAATGCGAACACGCCCGcccc                                                                      >  4:138955/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAACCCCCATTCGCCAGC‑AGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  8:6297/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCTGGCGTTACCCAACTTAATCGCCGTGCAGCACATCCCCCATTCGCCACC‑GGGCGGAATAGCGAAGAGGCCCGCACCg                                                                     >  1:46782/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCg                                                                     >  2:246952/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGCCGGGGGAAACCCAGGCGGTTCCCACCTTAATCGCCTTGGCGCCCAAACCCCATTAGCCACC‑AGGCGGAATAGCGCAGGGGCCCCCAccc                                                                     >  4:264835/1‑138 (MQ=255)

                                                                        cccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                    <  2:38957/139‑1 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACCACGTCGTTACAGGGAAAACCCATGCGTTAACCAACTTAATCGCCTTTCACCCCCTACCCCGTTCGCCCCC‑TGGCGGAATATCCAAGAGGACCGCCCCgca                                                                   >  6:359807/1‑137 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  3:149677/139‑1 (MQ=255)

                                                                              tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATACCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                              >  1:166519/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACACCGTCGTGACTGGGAAAACCCTGGCGTTAACCAACTTAATCGCCTTGCAGCACAACCCCCGTTCGCCCGC‑TGGCGTAATAGCGAAGAGGCCCGCCCCGCTCGCCCGTccc                                                         >  8:119959/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTACCCCACGTAATCGCCTTGCAGCACATCCCCCATTAGCCCCC‑TGGCGGTATAGCGAAGCGGCCCCCACCCCGAGCCCTTccc                                                         >  5:270164/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACATTATCGCCTTGGAGCACATCCCCCATTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCCCCCCCAGAGCCCTTccc                                                         >  6:260100/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCCATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         >  2:196826/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAag                                                                                 <  8:271302/115‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  1:87205/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACGTGGTAAACCCAGGCGTTTACCCACTTTATACCCGTGGCTCCCAACCCCCGTTCGCCAGC‑TGGCGGAATTGCGAAGAGGCCCGCGCCGCTCGCCCTTccc                                                         >  6:11218/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGGTTTACAACGTCCTGCCCGGGCTAACCCTGGCGTTCCCCCACATAAACGCCGTGGCGCACCTCCCCCGATCGCCAGCGTGGC‑TGATTGCGGAGCGGCCCGCCCCGCACGCCCGTccc                                                         >  5:203375/1‑139 (MQ=255)

                                                                                        gAATTCACTGGCCGTCGTTTTACAACGTCGTGTCAGGGAAAACCCAGGCGTTACCCCACTTAAAAGCCATGCAGCACATCCCCCATTCGCCAGC‑GGGCGTTAAATCCAAGAGGCCCCCACCCATCCCCCTGCCCcacac                                                    >  6:85462/1‑138 (MQ=255)

                                                                                              aCTGGCTGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  8:78998/139‑1 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGCAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCCGC‑GGGGGTAATAGCGAAGAGGCCCGCCCCGCTCGCCCGTCCCAACAGTTgcgc                                              >  6:62680/1‑139 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  6:137973/139‑1 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  5:167307/139‑1 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  1:227173/139‑1 (MQ=255)

                                                                                              aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑GGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  4:11980/139‑1 (MQ=255)

                                                                                                tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAag                                                                                 <  2:61198/102‑1 (MQ=255)

                                                                                                  gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCATGCAGCACCTCCCCCTTTCGCCAGC‑GGGCGTAATAGCGAAGCGGCCCTCACCGCGCGCCCTTACCAACAGTAGCGCAGcc                                          >  8:300421/1‑139 (MQ=255)

                                                                                                              cAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                              <  3:6554/139‑1 (MQ=255)

                                                                                                                   tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  7:356776/107‑1 (MQ=255)

                                                                                                                    cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                  >  3:347856/1‑66 (MQ=255)

                                                                                                                    cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                        >  1:100159/1‑139 (MQ=255)

                                                                                                                        acggggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGAt                    >  5:242826/4‑139 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  7:303463/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  8:179794/138‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAACAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  3:131604/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATACCCCTTTCGCCAGC‑TGGCGTAAAAGCGAAGAGGCCCGCACCGCTCGCCCGTCCCCCCAGTTGCGCAGCCTGAATGGCGCATGGCGCCTGATGCgg                >  2:327673/1‑139 (MQ=255)

                                                                                                                                  cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt          <  3:299987/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  4:322786/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  4:177931/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  5:348346/139‑1 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGATTGGCGCCTGATGCGGTATTTTCTcc      >  7:193130/1‑139 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 >  1:257222/1‑80 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc      >  7:165003/1‑139 (MQ=255)

                                                                                                                                       gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCTGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc      <  7:98262/138‑1 (MQ=255)

                                                                                                                                         gTTACGCAACTTAATCGCCTTGCAGCACATACCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCTGTATTTTCTCCTTa   <  7:22954/139‑1 (MQ=255)

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC‑TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`