BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,031	A→C	5.7%	A48A (GCA→GCC)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,031	0	A	C	5.7%	14351.9 / 118.4	5241	A48A (GCA→GCC)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2138/2694); new base C (374/17); total (2523/2718)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5971 total aligned reads displayed.

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGcccag                                                                                                                                         >  3:78018/1‑138 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCATGCGTTTCCCAAATTAATCGCCATGCAGCACAt                                                                                                                                         >  4:250191/1‑139 (MQ=255)

   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTCACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                                         >  4:100582/1‑139 (MQ=255)

    aatACGCCATGCTTGCATTCCTGCAGGTCGACTCTAGATGATCCCAGGGTACCGAGCTCGAATTCACCGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                                        <  5:259067/137‑1 (MQ=255)

     gtACGCCAAGCTTGCTTTCCTGCTGGTCGACTCTAGAGGATCCCCGGGGACCGAGCTCGAATTCACTGGCCGTCGTTATACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGACTTGCAGCACAtg                                                                                                                                        <  5:341960/137‑2 (MQ=255)

        cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGGTAACCCAGGCGTTTCCCCCCGTAATCGCCTTGGCGCACATccccc                                                                                                                                    >  3:305963/1‑139 (MQ=255)

         gCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGACGTCTATATACAAAGTCGTGACTTGTAAAACCCTCGCCATACCCAACATAAACGCCTTACACCACATccccca                                                                                                                                   >  8:238285/1‑138 (MQ=255)

          ccAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCCACTTAATCGCCTTGCAGCACATCCCCCtt                                                                                                                                  >  6:132158/1‑139 (MQ=255)

             aGCTTGCATTCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                                               <  8:343827/139‑1 (MQ=255)

             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                                               >  6:352800/1‑139 (MQ=255)

             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGGCGTGACTGGGTAAACCCTGGCGGTACCCAACTTAATCGCCTTGGCGCACCTcccccgtgag                                                                                                                               >  7:335357/1‑134 (MQ=255)

                  gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTGCCCCACGTAAACGCCGTGCAGCACATCCCCCTTTCGCCCGc                                                                                                                          >  8:160612/1‑139 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                          <  1:357740/138‑1 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                          <  6:179150/138‑1 (MQ=255)

                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTAGGAAAACCCTGGCGTTAACCAAAATAATAGCCTTGCAGCCCATCCCCCATTCGCCAg                                                                                                                           >  8:102244/1‑137 (MQ=255)

                       ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                     <  7:74845/139‑1 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCGTGCAGCACATCACCCATTAGCCAGCTGGCGt                                                                                                                    >  5:286053/1‑138 (MQ=255)

                         tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTGTTACAACGGCGGGGCGGGGTAAACCCGGGCGGTTCCCCACGTTATCGCCGGGGCGCCCCTCCCCCCTTCGCCCGCAGGCgg                                                                                                                    >  2:235343/1‑137 (MQ=255)

                            aGGTCGACTCTAGAGGATCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCATCTGGCGTAATa                                                                                                                <  2:128594/139‑1 (MQ=255)

                                 gACTCTAGAGGCTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  1:128181/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  6:270891/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  5:83968/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  2:172566/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  1:211950/139‑1 (MQ=255)

                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  1:26695/139‑1 (MQ=255)

                                 gACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  1:187279/139‑1 (MQ=255)

                                 gACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                           <  5:227791/139‑1 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                                             >  7:261432/1‑136 (MQ=255)

                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCGTGCCGCCCATCCCCCTTTACCCAGCTGGCGTAATAGCCAag                                                                                                          >  4:306625/1‑139 (MQ=255)

                                   ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACATAAACGCCTTGCAGCACATACCCCATTCGCCAGCAGGCGTAATTGCGAagc                                                                                                         >  2:5079/1‑138 (MQ=255)

                                   ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGGTAACCAACATAAAAGCCATTCAGCCCCTCCCCCGTTAGCCCCCAGGCGGAATATCGaa                                                                                                           >  4:167670/1‑137 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTTATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                               >  7:361800/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGcccc                                                                                               >  2:73819/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                     <  5:169573/117‑1 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                                             <  4:368082/125‑1 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                               >  1:93914/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACTTAATCGCCTTGCAGCACAACCCCCATTAGCCAGCGGGCCTAATAGCGAAGAGGCCCCCAcc                                                                                               >  2:206210/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCCAGGCGTTACCCAACTTAATAGCCTTGCAGCACATACCCCTTTAGCCAGCTGGCGTAATAGCGAATAGGCCCGCAcc                                                                                               >  4:190293/1‑139 (MQ=255)

                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGTCTGGGAAAACCCTGGCGGTTCCCAACTTAATCTCCTTGCAGCACCACCCCCAGTCGCCCGCTGGCCGAATAGCGCAGAGGCCCGcccc                                                                                               >  2:282723/1‑139 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGGTACCCAACATAATAGCCTTGCAGCCCCACCCCCGTTCGCCAGCAGGCGGAATAGCGAACAGGccccccccc                                                                                              >  3:251683/1‑133 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTTATAGCGAAGCGGCCCGCACCg                                                                                              >  8:78442/1‑139 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCg                                                                                                   <  2:30749/134‑1 (MQ=255)

                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGATTTACAACGTAGTGACTGGGAAAACCCTGGCGTTAACCAACTTAATCGCCTTGCAGCACATACCCCTTTCGCCACCTTGCGTAATAACGAATAGGCCAGCAcct                                                                                              >  4:149660/1‑138 (MQ=255)

                                                    gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                        <  6:324535/139‑1 (MQ=255)

                                                        cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCGTGCCGCCCCTCCCCCTTTCGCCAGCTGGCGTAAAAGCGAAGAGGCCCGCCCCCCTCGCCCGTc                                                                                    >  4:221896/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCCAAGAGGCCCGCACCGATCGCCCGTccc                                                                                  >  4:338503/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCATGGGAAAACCCTGGCGGTACCCCACTTAATAGCCTTGCAGCACCTCCCCCTTTCGCCCGCTGGCGGAATATCGAAGAGGCCCGCACCGCTCGCCCGTccc                                                                                  >  4:66375/1‑139 (MQ=255)

                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                  >  1:238221/1‑139 (MQ=255)

                                                            cTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCaa                                                                                <  3:124968/139‑1 (MQ=255)

                                                              cGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACa                                                                              <  6:77024/139‑1 (MQ=255)

                                                               gAATTCACTGGCCGTCCGTTTACAACGTCGTGACAGGGAAAACCCTGGCGTTACCCAACATAATCCCCATTCACCACCTCCCCCGTTCCCCAGCATGCGTAAATTCGAACAGGCCCGCACCCAAAGCACATCCCAACa                                                                              >  8:213314/1‑138 (MQ=255)

                                                                  ttgaCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTg                                                                          <  8:192464/136‑1 (MQ=255)

                                                                     gacagCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  5:202457/135‑1 (MQ=255)

                                                                       tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAg                                                                     >  3:333094/1‑139 (MQ=255)

                                                                              cgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCCACTTAATCGCCGTGCCGCCCAGCCCCCATTAGCCAGCTGGCGGAATAGCGAAGAGGCCCCCCCCGCTCGCCCGTCCCACCCGTTGCGCCGCCAga                                                                >  4:94642/1‑137 (MQ=255)

                                                                                        cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                                                    >  7:308949/1‑139 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  6:45726/118‑1 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCCGCCGGAATGGCGAATGgcgc                                                  >  7:145089/1‑139 (MQ=255)

                                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCACCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCCGCCTgaaatgcgaatggccc                                                  >  1:51523/1‑137 (MQ=255)

                                                                                           cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                                                 >  3:43117/1‑139 (MQ=255)

                                                                                           cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                                                 <  1:323952/139‑1 (MQ=255)

                                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCACTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATgag                                          <  4:126636/138‑3 (MQ=255)

                                                                                                    gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                                     <  8:293187/94‑1 (MQ=255)

                                                                                                             ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                                          <  2:257222/80‑1 (MQ=255)

                                                                                                             ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                               >  7:217643/1‑139 (MQ=255)

                                                                                                             ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                               >  6:209541/1‑139 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          <  7:118808/138‑1 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  8:126871/1‑138 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          >  1:10/1‑138 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGc                         >  5:56285/1‑139 (MQ=255)

                                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          <  5:312677/138‑1 (MQ=255)

                                                                                                                         cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatgg                                                     >  8:258577/1‑105 (MQ=255)

                                                                                                                         cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                            >  1:70700/1‑130 (MQ=255)

                                                                                                                          ttAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGACCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  8:369605/139‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  8:320411/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  6:96961/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  5:120905/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  7:170063/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  3:169178/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  1:159577/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  5:365260/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACATCCCCCATTCGCCAGCTGGGGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  7:199681/137‑1 (MQ=255)

                                                                                                                            aaTCGCCTTGCAGCACAACCCCCATTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  3:279295/137‑1 (MQ=255)

                                                                                                                             agcGCCTTGCAGCAGATCCCCCTTTCTCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAGTTTCTCCTTACGCATCTGTGCg                <  3:147332/136‑1 (MQ=255)

                                                                                                                             aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  1:294455/138‑1 (MQ=255)

                                                                                                                             aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  7:51335/138‑1 (MQ=255)

                                                                                                                              tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  4:278427/82‑1 (MQ=255)

                                                                                                                              tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg               >  3:359930/1‑138 (MQ=255)

                                                                                                                                 ccTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                   <  6:239531/115‑1 (MQ=255)

                                                                                                                                   ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  7:239590/106‑1 (MQ=255)

                                                                                                                                   ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGAGCAGCCTGAATGGCGTATGGCGCCTGATGCGGTATTTTCTCATTACGCATCTGTGCGGTAttt          >  2:34773/1‑138 (MQ=255)

                                                                                                                                     gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca        <  6:106851/138‑1 (MQ=255)

                                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcaca      <  7:285408/139‑1 (MQ=255)

                                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  5:144583/138‑1 (MQ=255)

                                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:155494/138‑1 (MQ=255)

                                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  8:55948/138‑1 (MQ=255)

                                                                                                                                      caccacaTCCCCTTTGCGCCAGCTGGGGTAATAGGCTACAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGACTGAATGGCTAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  3:224935/135‑1 (MQ=255)

                                                                                                                                       agcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCACCAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCacac     <  3:195183/139‑1 (MQ=255)

                                                                                                                                       agcaCATCCCCCTTTCGCCAGATGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCacac     <  6:300554/139‑1 (MQ=255)

                                                                                                                                          acaTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGc  <  2:98870/139‑1 (MQ=255)

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`