BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,256	A→C	5.4%	intergenic (+45/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,256	0	A	C	5.4%	12609.0 / 96.5	5287	intergenic (+45/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (2389/2449); new base C (352/11); total (2813/2474)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5386 total aligned reads displayed.

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

gcgcCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACTGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGa                                                                                                                                            <  2:989/139‑1 (MQ=255)

    cTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCTGACggg                                                                                                                                        <  5:214747/139‑1 (MQ=255)

      gATGCGGTAGTTTCTCCTTACGCATCTGTGCGGTAGTTCACACCGCATATGGTGCACTCTCAGTACACTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCt                                                                                                                                      <  8:152464/139‑1 (MQ=255)

            gTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTg                                                                                                                                >  3:92193/1‑139 (MQ=255)

             tATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCtt                                                                                                                                >  8:265379/1‑137 (MQ=255)

               ttttCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCt                                                                                                                              <  7:70899/138‑1 (MQ=255)

                      cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCAt                                                                                                                      <  1:100604/139‑1 (MQ=255)

                         aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCg                                                                                                                   >  3:229729/1‑139 (MQ=255)

                         aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCATCCg                                                                                                                   >  5:269336/1‑139 (MQ=255)

                          cGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGc                                                                                                                  <  6:219177/139‑1 (MQ=255)

                           gCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCAGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCGGCATCCGCt                                                                                                                 <  7:329018/139‑1 (MQ=255)

                               cTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCGGACGGGGTTGTCTGCTCCCGGCCTCCGCTTa                                                                                                               >  3:171614/1‑137 (MQ=255)

                                tgtgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACCCCCGCTGACGCGCCCGGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAg                                                                                                            >  4:314711/1‑139 (MQ=255)

                                  tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACCCCCGCTGCCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                          >  1:216048/1‑139 (MQ=255)

                                  tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           <  4:89379/138‑1 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           >  1:191257/1‑133 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCATCCCCGTTCAGACCAGCt                                                                                                     >  2:361507/1‑139 (MQ=255)

                                         tttCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCtgt                                                                                                   >  7:285820/1‑139 (MQ=255)

                                           tCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 >  1:237921/1‑139 (MQ=255)

                                            cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                     <  3:345635/118‑1 (MQ=255)

                                             acacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAc                                                                                                <  2:206310/138‑1 (MQ=255)

                                              cacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                              >  2:223096/1‑139 (MQ=255)

                                              cacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                              <  6:307526/139‑1 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAc                                                                                                <  3:277638/134‑1 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTTCTCCCGGCCTCCGCTTACAGACAAGCGGGGACCCGCTcc                                                                                         >  1:162881/1‑139 (MQ=255)

                                                    atatGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCg                                                                                        <  4:266/139‑1 (MQ=255)

                                                     tatGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           >  6:163613/1‑119 (MQ=255)

                                                         gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                    <  7:46661/138‑1 (MQ=255)

                                                         gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                    <  7:95067/138‑1 (MQ=255)

                                                         gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                    <  8:75359/138‑1 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCACCACCCGCTGACGCGCCCGGCCGGGGGTGTCGGCTCCCGGCATCCGCTTACAGACCAGCTGTGCCCGGCTCCGGGAGCTg                                                                                 >  5:80063/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGGTTGTCTGCTCCCGGCCTCCGCTTACAGGCAAGCTGTGACCGTCGCCGGGAGCTg                                                                                 >  1:133152/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           <  8:209341/113‑1 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                 >  1:56719/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCATCCCCATACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                 >  2:54353/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCGTGTCTGCTCCCGGCAACCCCTTACAGACAAGCAGTGACCGTCTCCGGGGGcgg                                                                                 >  3:202478/1‑137 (MQ=255)

                                                             aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGTCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                               <  6:254615/139‑1 (MQ=255)

                                                             aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGa                                                                                                                                            <  8:184292/78‑1 (MQ=255)

                                                             aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                               <  5:56248/107‑1 (MQ=255)

                                                              ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAt                                                                              >  5:315322/1‑139 (MQ=255)

                                                              ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCGTTTCTGCTCCCCGCATACGCTTACAGCCAAGCTGTGAACCTCTCCGGGCGCGGCAt                                                                              >  2:168166/1‑139 (MQ=255)

                                                               tctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTACCCGCATCCGCTTACAGACCAGCTGTGACCGTCGCCGGGAGCGGCAtg                                                                             >  4:210217/1‑139 (MQ=255)

                                                                 tcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAg                                                                                                       >  8:239823/1‑111 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                               >  8:332320/1‑100 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCAGTGCCCGTCTCCGGGAGCGGCATgtgt                                                                          >  3:68245/1‑137 (MQ=255)

                                                                      aCAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATACGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCag                                                                       <  5:361380/138‑1 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTTACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  2:246417/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  5:161381/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCGCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  3:373282/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCCGGCTTGTCTTAACCCGGGATACGCGAAAACACAAGCTGTGACCCAGGCCCGGACGTGCATGGTTCAGAGGt                                                                   >  8:49249/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTGTCTGCTCCCGGCCTCCGCATACAGACAAGCTGTGACCGTCTGCGGGAGCTGCCTGTGTCAGAGGt                                                                   >  2:71765/1‑139 (MQ=255)

                                                                          tCTGCTCTGATTCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGCCGGGCTTGTCTGCTCCCGGCATc                                                                                                                     >  3:296719/1‑88 (MQ=255)

                                                                          tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGTCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGCCCGTCGCCGGGAGCTGCATGTGTCAGAGGtt                                                                  >  3:253550/1‑139 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCCGCATCCCCTTTCAGCCAAGCTGTGACCGGCGCCCGGCGCGGCCTGTGTCAGCGGtttt                                                                >  4:340559/1‑138 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACa                                                                                                             <  2:131707/93‑1 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           <  3:149658/95‑1 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                               >  2:281764/1‑123 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtttt                                                                >  1:76626/1‑138 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTg                                                                                                                                <  1:298537/72‑1 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACCAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             >  7:244589/1‑139 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCg                                                                                        <  4:59615/112‑1 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                        >  7:218948/1‑128 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCGGGCTTGTCTGCTCCCGGCCTCCGCGTACAGCCAAGCGGTGACCGTCTCCGGGAGCGGCATGTGTCAGAGGttttacc                                                             >  4:178882/1‑136 (MQ=255)

                                                                                 tGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                             <  7:111723/137‑1 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          >  7:264454/1‑138 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  4:289010/99‑1 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGtc                                                         >  4:240702/1‑139 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCCTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          >  3:321492/1‑138 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAc                                                    >  6:185593/1‑139 (MQ=255)

                                                                                         cATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAcc                                                   >  1:268476/1‑139 (MQ=255)

                                                                                         cATAGTTAAGCCAGCCCCGACACCCGCCAACACACGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCCTACGCTTACAGACAAGCAGTGACCGTCGACGGGAGCTGCATGTGTGAGAGGTTTTCCCCGTCCTCAcc                                                   >  4:364169/1‑139 (MQ=255)

                                                                                             gTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTTCTCCCCGCATCCGCTTACAGACAAGCTGTGACCGGCTCCCGGGGCTGCATGTGTCAGAGGTTTTCACCGTGATCACCGcaa                                               >  6:51122/1‑139 (MQ=255)

                                                                                              ttAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACCGGCGTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCCTGTGTCAGAGGTTTTCACCGTCATCACCCAAAc                                              >  5:294810/1‑139 (MQ=255)

                                                                                                 cgccggccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcg                                           <  1:327330/138‑1 (MQ=255)

                                                                                                   ccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCGTACAGCCAAGCGGTGACCGTCGCCGGGAGCGGCATGTGTCAGAGGTTTTAACCGTCATGCCCGAAAcgcgcg                                         >  7:196249/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  5:339659/80‑1 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACACACAAGCTGTGACCGTCGCCGGGGGCTGCATGTGTCAGAGGTTTTGACCGTCATCACCGAAACGCGCgaga                                      >  5:186487/1‑139 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGtca                                                        >  7:306895/1‑120 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTGTATGCTACCCGCCACCCCTTACAAACAACCAGTGACCCTCACCGGGCGCGGCAAGTGTCACCGGTTTTCACCGTAAACACCCAAACCCGCGAGAc                                     >  2:108055/1‑139 (MQ=255)

                                                                                                           gACACCCGCCAACACCCGCTGACGCGCCCTGACGGGAGTGTCTGCTCCCGGAATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTAACCGTCATCACCGAAACGCGCGAGACGaaa                                 <  6:281302/139‑1 (MQ=255)

                                                                                                            acacCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAg                                >  7:168870/1‑139 (MQ=255)

                                                                                                             cacCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAgg                               <  3:123983/139‑1 (MQ=255)

                                                                                                                ccGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                            <  6:111563/139‑1 (MQ=255)

                                                                                                                 cGCCAACCACCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCATACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGCGACGAAAGGGCCt                           <  4:123180/139‑1 (MQ=255)

                                                                                                                 cGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                           <  7:136752/139‑1 (MQ=255)

                                                                                                                  gCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCCCCGAAACGCGCGAGACGAAAGGGCCTc                          >  7:353458/1‑139 (MQ=255)

                                                                                                                  gCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCACCCGGCCACCGCTTACAGACAAGCTGTGACCGGCTCCCGGAGCGGCATGTGTCAGAGGTTTTCACCGTAATCACCGAAACCCCCCAGACGAAAGGGCCTc                          >  2:319114/1‑139 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  8:35811/139‑1 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  8:128982/139‑1 (MQ=255)

                                                                                                                          ccGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGGCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                  <  8:307195/139‑1 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                <  5:245122/139‑1 (MQ=255)

                                                                                                                             cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                >  5:139599/1‑138 (MQ=255)

                                                                                                                                   cgcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAttttt         <  1:36748/139‑1 (MQ=255)

                                                                                                                                   cgcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAttttt         <  6:337042/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACTGTCTTGTCTGCTCCCGGCAACCGCTTACATACAAGCTGTGACCGTCTCCGGGAGCTTCTTGTGTCAGAGGTTTTCACCGGCATCACCGAAACGCGAGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  5:11310/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGGATGTGTCAGAGGTTTTGACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  6:356/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  6:13766/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGGGACCGTCTCCGGGAGCTGCATGTGGCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  6:78556/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGGCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  8:28834/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGACTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  4:150113/139‑1 (MQ=255)

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`