| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,379,782 | T→C | 13.6% | R226R (CGT→CGC) | U712_07055 → | Ktr system potassium uptake protein D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,379,782 | 0 | T | C | 13.6% | 146.3 / 15.7 | 59 | R226R (CGT→CGC) | U712_07055 | Ktr system potassium uptake protein D |
| Reads supporting (aligned to +/- strand): ref base T (30/21); new base C (5/3); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 | |||||||||||
GTAATTCGATGATTCCATTTCGCCACGATTACTTTGTGCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATATTTTGTTTTTGTCC > CP006881/1379652‑1379920 | gTAATTCGATGATTCCATTTCGCCACGATTACTTTGTGCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAATCATCGGCGTTATCCAtt > 3:191435/1‑139 (MQ=255) ccATTTCGCCACGATTACTTTGTGCAGATTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCCTTTACGCTTTTTACa < 7:196342/138‑1 (MQ=255) ccACGATTACTTTGTGCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCac > 4:329321/1‑138 (MQ=255) cACGATTACTTTGTGCAGTTTTTTACAATGCTTTTAATTATATTTGGAGCGAGTGGATTTCCCGTGCTGGTGGCAGTACAGGATTTCCGGTGTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCacga < 4:324984/139‑1 (MQ=255) ttACTTTGTGCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCACGACGAtt < 8:314989/139‑1 (MQ=255) tgtgCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGtt < 5:95776/138‑1 (MQ=255) tgtgCAGTTAATTACAATGCTTTTAATTATATTTGGAGCGAATGGTTTGCCCGGGCTTGTTGAAGTAACGCATGTCCTGTTTTCAAACCATCGGGGTTATCCATTTACTCTTTTTACAAATATCACGACGATTACGtt < 1:231117/138‑1 (MQ=255) tgCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCTTTTACGCTTTTTACAAAGATCACGACGATTACGTTCgg > 1:372792/1‑139 (MQ=255) tgCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACTAAGATCACGACGATTACGTTCgg > 8:149238/1‑139 (MQ=255) tgCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCacga > 1:45999/1‑126 (MQ=255) tgCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCacga < 2:45999/126‑1 (MQ=255) agaggTTTATTAAAATGCTTTTAATTATATTTGTAGCGATTGGATTTCCCGTGATTGTTTAATTAAAGGATTTCCTGTTTTCAAAGCATCGTAGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCgg < 3:150137/135‑1 (MQ=255) tttATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGAGTTCCTGTTGTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCAc > 6:279014/1‑139 (MQ=255) taCAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCAc > 4:349047/1‑134 (MQ=255) taCAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCAc < 3:349047/134‑1 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 4:276334/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 7:106544/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 7:313988/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 7:364041/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 1:56916/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 1:42832/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 1:310006/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGtt > 5:107176/1‑139 (MQ=255) tGCTTTTAATTATATTTGGAGCGATTCTATTTCCCGTGCTAGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGTCTCActtgtctctgt > 4:150137/1‑135 (MQ=255) cTTTTAATTATATTTGGAGCGGTTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCAc < 7:71779/127‑1 (MQ=255) cTTTTAATTATATTTGGAGCGGTTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCAc > 8:71779/1‑127 (MQ=255) cTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACa > 6:331441/1‑99 (MQ=255) cTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACa < 5:331441/99‑1 (MQ=255) cTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCac > 4:68298/1‑107 (MQ=255) cTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCac < 3:68298/107‑1 (MQ=255) ttAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTa < 2:324434/77‑1 (MQ=255) ttAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTa > 1:324434/1‑77 (MQ=255) ttAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAg > 4:367060/1‑139 (MQ=255) tatTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACa > 8:102484/1‑89 (MQ=255) tatTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACa < 7:102484/89‑1 (MQ=255) tatTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTgg > 6:70181/1‑139 (MQ=255) tatTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTgg > 6:309000/1‑139 (MQ=255) atTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTgg < 1:71987/138‑1 (MQ=255) gAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCt < 8:165737/138‑1 (MQ=255) aTTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGccc < 5:53217/139‑1 (MQ=255) aTTGGATTTCCCGTGCTGGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGccc < 7:32770/139‑1 (MQ=255) ttGGATTTCCCGTGCTTGTGGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCt < 6:154888/139‑1 (MQ=255) ggATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTg < 8:364041/138‑1 (MQ=255) ttgAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGtt < 8:106544/139‑1 (MQ=255) ttgAAGTAAAGGATTTCCTGTTTTAAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGtt < 5:331591/139‑1 (MQ=255) gTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACGTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCCTTCGAAg > 4:141374/1‑139 (MQ=255) aaaGGATATCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAgg > 8:370606/1‑138 (MQ=255) gATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAAt < 5:70181/139‑1 (MQ=255) tttCCTGTTTTCAAAGCATCGGCGCTATCCATTTACGCATTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTATGGACCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTAGAAGGAAAATCa > 7:71081/1‑139 (MQ=255) tCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATg > 2:86796/1‑139 (MQ=255) ccTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATgg > 7:182205/1‑139 (MQ=255) ttttCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATg < 3:329321/139‑1 (MQ=255) ttCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGat > 3:261125/1‑139 (MQ=255) ttCAAAGCAACGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGa > 1:356543/1‑138 (MQ=255) aGCATCGGCGCTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATAtttt > 8:40450/1‑139 (MQ=255) aTCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAAt > 4:348813/1‑120 (MQ=255) aTCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAAt < 3:348813/120‑1 (MQ=255) tCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATAttttgttt > 2:11419/1‑139 (MQ=255) cGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATAttttgtttt > 5:99193/1‑139 (MQ=255) ttATCCATTTACGCTTTTTACAACGATCACGACGATTACGTTCGGCTCACGTGTCGTGTTTTGAGCCATTGGCATCTTAGCACTGGAAGCAAACCATGCGTTCGCAGGAAAATCATTGCATGATATTTTGTTTTTGTcc < 1:28469/139‑1 (MQ=255) ttATCCATTTACGCTTTTTACAAATATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATATTCTGTTTTTGTcc < 4:191435/139‑1 (MQ=255) ttATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATATTTTGTTTTTGTcc < 2:310006/139‑1 (MQ=255) | GTAATTCGATGATTCCATTTCGCCACGATTACTTTGTGCAGTTTATTACAATGCTTTTAATTATATTTGGAGCGATTGGATTTCCCGTGCTTGTTGAAGTAAAGGATTTCCTGTTTTCAAAGCATCGGCGTTATCCATTTACGCTTTTTACAAAGATCACGACGATTACGTTCGGCTCACTTGTCTTGTTTGGAGCCATTGGCATCTTTGCCCTGGAAGCAAACCATGCGTTCGCAGGAAAATCATGGCATGATATTTTGTTTTTGTCC > CP006881/1379652‑1379920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |