Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	230,494	A→C	17.8%	N322T (AAC→ACC)	U712_01065 →	Fatty‑acid peroxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	230,494	0	A	C	17.8%	96.8 / 2.1	56	N322T (AAC→ACC)	U712_01065	Fatty‑acid peroxygenase
Reads supporting (aligned to +/- strand):  ref base A (33/11);  new base C (2/9);  total (36/20)
Fisher's exact test for biased strand distribution p-value = 8.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAT  >  CP006881/230358‑230632                                                                                                                                         |                                                                                                                                           gaAATGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAAcc                                                                                                                                          >  1:342026/1‑139 (MQ=255)   tttGTTTGGGCTGGCGGTCCGCATTTATTAGCCGGGGGGCCCGTTTTTTGGGGCGCTTTTCAAAAATTTTTTTTTTTGTAATAACTGTGAGTTTAACACGGCCACAGCGGTGGTGCTTTATTTATATGCAACCACCCa                                                                                                                                         <  2:342026/136‑1 (MQ=255)   aaTGTTTGTGCTGGTGGGCCGCAGATATTATCCGTGCGGCCGGTTTTTCGGGGCGCTTGTCAAAAATTTTTTTTTATGGAATAACTGTGAGTTTAAGACGGGCACATGGGTGCTGCTTTATTTATATGCAaccaacca                                                                                                                                         <  2:353825/138‑1 (MQ=255)   aaTGTTTGTGCAGGAGGGCCGCATATATTATCCGTTCGGCCCTTTTTTCGGGGCGCTTGTCAAAAAAGATTTTGTATGTAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCa                                                                                                                                         <  6:151354/138‑1 (MQ=255)    aTGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACg                                                                                                                                       >  2:264748/1‑139 (MQ=255)           tgCAGGAGGTCCGCATATATTATCCGTTCGGCCGGTTTTTAGGGGCGCTTGACAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTc                                                                                                                                 <  2:216579/138‑1 (MQ=255)            gCAGGTGTTCCGCATATATTATCCGGTCGGCCGGTTTTTTGGGGCGCTTTTAAAAAAATTTTTTTTATGTAATAACTGTGTGTTTAAGATGGGCACAGCGGTGCTGCTTTATTTATATGGAACGAACCACGACCCTCGt                                                                                                                               <  4:284556/139‑1 (MQ=255)                   gTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATggg                                                                                                                        >  3:242707/1‑139 (MQ=255)                     ccGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGat                                                                                                                      >  3:284556/1‑139 (MQ=255)                        cAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGatcat                                                                                                                   >  4:164486/1‑139 (MQ=255)                             attatCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGa                                                                                                              >  6:127342/1‑139 (MQ=255)                             attatCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGa                                                                                                              >  5:126445/1‑139 (MQ=255)                                atCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCCCATCGGGGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATg                                                                                                            >  4:253859/1‑138 (MQ=255)                                atCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATg                                                                                                            >  7:293474/1‑138 (MQ=255)                                     ttCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTcc                                                                                                      >  3:349866/1‑139 (MQ=255)                                                        cgcTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCg                                                                                     >  1:128664/1‑137 (MQ=255)                                                        cgcTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCg                                                                                     >  1:2179/1‑137 (MQ=255)                                                             tcaaaaaacTATTTTGTTTTGAATATCTTTGAGTTATACAAGGGCCAAGGGGGGGTGTTTTATTTATATGGACCCACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCg                                                                                <  6:280780/134‑1 (MQ=255)                                                               aaaaaaaGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGGGCTGCTTTATTTATATGGAACCAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGgaa                                                                            <  3:164486/138‑1 (MQ=255)                                                                  aaaaGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCTGTGATGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATCGGATAATCCCGAAGAATTCCGGCCGGAACGATTTGCGGAGCGGgaaga                                                                          >  3:354466/1‑138 (MQ=255)                                                                     attttttttATGTATTACCTGTGTGTTTAAGACGGGCACTGCGGGGCTGTTTTATTTATATGCAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAAt                                                                      <  2:128664/136‑1 (MQ=255)                                                                     aGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAAt                                                                      <  6:126445/139‑1 (MQ=255)                                                                              aTGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCGGATTTATTTATATGCAACCAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGa                                                              <  5:96351/138‑1 (MQ=255)                                                                                  aaTAACTGTGCGTTTACGAAGGGCACATCGGTGCTGCTTGATTTAAATGGACCCAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGa                                                         <  2:293878/139‑1 (MQ=255)                                                                                    tAACTGTGTGTTTAAGACGGGCACTTGGGTGCTGCTTGATTTATATGCACCGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGAtt                                                       <  8:144055/139‑1 (MQ=255)                                                                                          tgAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTc                                                   <  8:293474/137‑1 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  1:131896/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  3:355807/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  3:82431/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  5:321892/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  5:24740/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  5:192164/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  7:121584/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAgg                                               >  7:264493/1‑139 (MQ=255)                                                                                            aGTTTAAGAAGGGCACATCGGTGCTGCTGGATTTATATGGAACGAACCACGACCCTCGTCTATGCGATCATCCCGAATAAATCCGGCAGGAACGATTAGCGGAGCGGGAAGAAAAGCTGTATGATATCATTCCACaacg                                               >  1:97048/1‑137 (MQ=255)                                                                                                   gatgggCACTTGGGTGCTTGTTTATTTTTATGCACCCCACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCgggggg                                        <  2:318990/136‑1 (MQ=255)                                                                                                      gggCACATCGGTGCTGCTTGATTTATATGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCAc                                     >  5:232346/1‑139 (MQ=255)                                                                                                      gggCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGATCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCAc                                     >  5:91072/1‑139 (MQ=255)                                                                                                       gtcactTCGGTGCTGCTTTCTTTTTAAGGACCGTCCCACGACCCTCGTCTATGGGATCATCCCGATGAATGCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGTTTCCTCAAGGCGGGGGGCACg                                    <  8:210955/137‑1 (MQ=255)                                                                                                         cacaTCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAAGCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGcc                                  >  4:203544/1‑139 (MQ=255)                                                                                                            aTCGGTGCTGCATGATTTATATGGAACGCACCACGACCCTCGTCTATGGGATCATCCCGATGAATACCGGCCGGAACGATATGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGTGCTCGcc                                  >  6:290591/1‑136 (MQ=255)                                                                                                                 tgctgcTTGATTTATATGGAACGAACAACCACCCACGTCTATGGGATCATCCCGCTGAATTCCTGCCCGAACGATTTGCGGAGCGCGAAGAACATCTCTTAGAGATGATTCCTCAAGGCGGAGGGCACTCAGAGAACgg                          >  2:305488/1‑139 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGCTTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCAcc                     >  3:282532/1‑139 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGACCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCAc                      <  1:6693/138‑1 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCAcc                     >  7:1979/1‑139 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCAc                      >  2:6693/1‑138 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATACCGATGAATTCCGGCCGGAACGATTTGAGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGaccacc                     >  7:264817/1‑139 (MQ=255)                                                                                                                      ctTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATAATCCCTATGAATTCCGGCCGTAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTACTCAAGGCGGGGGGCACGCCGAGAAAGGCCAcc                     >  7:312854/1‑139 (MQ=255)                                                                                                                       tttatttatttGCAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCg                    <  4:82431/136‑1 (MQ=255)                                                                                                                       ttGATTTATATGCACCCAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCg                    <  4:349866/139‑1 (MQ=255)                                                                                                                       gttagttATATGTCACCACCCCCGACCCTCGTCTATGGTCTCATCCCGATGAATTCCGGCCGGAACGATTGGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCg                    <  8:354071/134‑1 (MQ=255)                                                                                                                        ttttttATATGTACCCAACCACGACCCTCGTTTATGGGATCATCCCGTTTAATTCCGGCCGGATCGATTTGGGTAGCGGGAATAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGATAAAGGCCACCg                    <  8:264817/135‑1 (MQ=255)                                                                                                                           tttATATGGCACGATCCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGt                >  7:121911/1‑139 (MQ=255)                                                                                                                           tttATATGGACCGACCCACGACCCTCGTCTATGGGAGCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTg                 <  4:318536/138‑1 (MQ=255)                                                                                                                           tttATATGGACCGAACCACGCCCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGt                >  8:18755/1‑139 (MQ=255)                                                                                                                           tttATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGt                >  4:353082/1‑139 (MQ=255)                                                                                                                           tttATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGt                >  2:60876/1‑139 (MQ=255)                                                                                                                                  cgaaccaacCACGACCCTCTTCTATGGGATCATCCCGATGAATTCCGGGCTGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATTTTATTCCTCAAGGCGGGGGGCACGCCGAGAAAGTCCACCGCTGTCCAggg          <  1:305488/137‑1 (MQ=255)                                                                                                                                    ttcccacCACGACCCTCGGGTATGGGATCAACCCTATTAATTCCGGGCGGAACGATTGGCGGAGCGGGAAGAAAATCTGTTTGATATCATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGaa       <  8:121911/134‑1 (MQ=255)                                                                                                                                    aacgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCGCGCCGAGAAAGGCCGCCGCTGTCCAgggg         >  1:273826/1‑137 (MQ=255)                                                                                                                                    aacgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAgggg         >  5:152483/1‑137 (MQ=255)                                                                                                                                      cgaacCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGAGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGCCGGGGGCCACGCCGACAAAGGCCACCGCTGTCCAGGGGAAgg     >  6:154023/1‑139 (MQ=255)                                                                                                                                         acCACGACCCTCGTCTATGGGATCCTCCCGATGAATTCCGGCCGGAACGAGTTGCGGAGCGGGAAGAAAATCTGTTTGAGATGATTCCCCACGGCGGGGGGCACGCCGACAACGGCCCCCGCGGTCCCGGGGAAGGCAt  >  4:58567/1‑139 (MQ=255)                                                                                                                                         acCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGTTTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAt  <  2:2179/139‑1 (MQ=255)                                                                                                                                         acCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAt  <  1:35725/139‑1 (MQ=255)                                                                                                                                         acCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAt  <  8:121584/139‑1 (MQ=255)                                                                                                                                         acCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAt  >  7:144423/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                           GAAATGTTTGTGCAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGATTTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGAACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCGGAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGCCACCGCTGTCCAGGGGAAGGCAT  >  CP006881/230358‑230632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑