Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,845,945 T→C 5.3% intergenic (+76/‑259) U712_09185 → / → U712_09190 Uncharacterized protein ynaC/putative N‑acetyltransferase YnaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,845,9450TC 5.3% 235.8 / 3.4 76intergenic (+76/‑259)U712_09185/U712_09190Uncharacterized protein ynaC/putative N‑acetyltransferase YnaD
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base C (2/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

AGCCAATACGAAGTTGAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTGCTTGTTCACAGT  >  CP006881/1845807‑1846083
                                                                                                                                          |                                                                                                                                          
aGCCAATACGAAGTTGAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCttt                                                                                                                                            >  4:157766/1‑139 (MQ=255)
        agAAGTTGAAATTTCACTTCTTAAAAAGCTTAATAATGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCt                                                                                                                                     <  6:161899/137‑1 (MQ=255)
           aGTTGAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCt                                                                                                                                 >  5:23838/1‑139 (MQ=255)
           aGTTGAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCt                                                                                                                                 >  4:166973/1‑139 (MQ=255)
               gAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTAc                                                                                                                              <  5:216752/138‑1 (MQ=255)
                aaaTTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTAc                                                                                                                              >  7:284628/1‑137 (MQ=255)
                        cttcttAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATc                                                                                                                    <  7:229636/139‑1 (MQ=255)
                                aaaGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTaa                                                                                                            <  2:176256/139‑1 (MQ=255)
                                 aaGCTTAATAAGGAATAACAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTaaa                                                                                                           <  6:288623/139‑1 (MQ=255)
                                 aaGCATAATAAGGAAAACCAATTAAATTAAACAATCTTGTTGGAAACTAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTaaa                                                                                                           <  2:16874/139‑1 (MQ=255)
                                              aaTACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCg                                                                                              >  5:251974/1‑139 (MQ=255)
                                              aaTACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCg                                                                                              >  8:47321/1‑139 (MQ=255)
                                                 aCCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCt                                                                                           >  6:233268/1‑139 (MQ=255)
                                                     attaaattaaACAATCTTGTTTTTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTAAg                                                                                       >  4:326507/1‑139 (MQ=255)
                                                      ttaaattaaaCAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGt                                                                                      >  1:197222/1‑139 (MQ=255)
                                                       taaattaaaCAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGtt                                                                                     >  3:175264/1‑139 (MQ=255)
                                                              aaCAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCa                                                                               >  7:109900/1‑138 (MQ=255)
                                                                   tCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAAt                                                                          >  6:37934/1‑138 (MQ=255)
                                                                     ttgttgGTAACAAACATCCTCTTACTTATTGTCAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCTGCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTcc                                                                       <  4:48748/139‑1 (MQ=255)
                                                                     ttgttgGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTcc                                                                       <  8:320681/139‑1 (MQ=255)
                                                                       gttgGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTccc                                                                      >  5:225521/1‑138 (MQ=255)
                                                                       gttgGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTccc                                                                      <  7:75422/138‑1 (MQ=255)
                                                                       gttgGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTccc                                                                      >  7:170525/1‑138 (MQ=255)
                                                                             aacaaacaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTcc                                                                       >  1:146813/1‑131 (MQ=255)
                                                                             aacaaacaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTcc                                                                       <  2:146813/131‑1 (MQ=255)
                                                                             aacaaacaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTa                                                               >  5:95501/1‑139 (MQ=255)
                                                                              acaaacaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCGTTAGTTTTGTCATAAATTCCCGATATTAc                                                              >  5:198006/1‑139 (MQ=255)
                                                                               caaacaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTAcc                                                             >  6:73575/1‑139 (MQ=255)
                                                                                  acaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          <  7:340859/139‑1 (MQ=255)
                                                                                  acaTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          <  5:173016/139‑1 (MQ=255)
                                                                                    aTCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCa                                                        <  6:188702/139‑1 (MQ=255)
                                                                                      ccTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGCTCTTACTTCGGCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCa                                                        >  6:166911/1‑137 (MQ=255)
                                                                                      ccTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCa                                                        >  1:99455/1‑137 (MQ=255)
                                                                                      ccTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCa                                                        >  5:22809/1‑137 (MQ=255)
                                                                                            acttaTTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGa                                                >  6:94352/1‑139 (MQ=255)
                                                                                             cttaTTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGa                                                <  5:15168/138‑1 (MQ=255)
                                                                                                aTTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAgg                                            >  7:154522/1‑139 (MQ=255)
                                                                                                aTTGTAAGAGGATGTTTGTTTAATTCATACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCAACCACAGTAAATGATCTTACTTCGTCTTAAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAgg                                            >  2:62716/1‑139 (MQ=255)
                                                                                                  tGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     >  1:173584/1‑128 (MQ=255)
                                                                                                  tGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     <  2:93827/128‑1 (MQ=255)
                                                                                                  tGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     <  2:173584/128‑1 (MQ=255)
                                                                                                  tGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     >  1:93827/1‑128 (MQ=255)
                                                                                                     aaGATGATGTTTTTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGa                                        <  6:245654/138‑1 (MQ=255)
                                                                                                      agagGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          <  5:217634/119‑1 (MQ=255)
                                                                                                      agagGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          >  6:217634/1‑119 (MQ=255)
                                                                                                      agagGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGatt                                      >  8:195647/1‑139 (MQ=255)
                                                                                                      agagGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGat                                       >  7:98745/1‑138 (MQ=255)
                                                                                                      agagGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGat                                       >  2:73127/1‑138 (MQ=255)
                                                                                                           aTGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTAtttt                                 >  1:279956/1‑139 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTg                                >  4:145653/1‑139 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCAAATTACCCCTCAGAGAAGGATAGGGGGATTATTTTg                                >  7:334857/1‑139 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTCGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     <  7:73172/118‑1 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTCGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     <  7:79089/118‑1 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTCGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     >  8:73172/1‑118 (MQ=255)
                                                                                                            tgtttgttTAATTCTTACATATTTTATCTTCGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCagag                                                     >  8:79089/1‑118 (MQ=255)
                                                                                                              tttgtttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACTGTAAATCATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGga                              <  6:132294/139‑1 (MQ=255)
                                                                                                              tttgtttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTgag                              <  7:47321/139‑3 (MQ=255)
                                                                                                              tttgtttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGga                              <  1:73127/139‑1 (MQ=255)
                                                                                                              tttgtttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGga                              <  7:195647/139‑1 (MQ=255)
                                                                                                                  tttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCaga                                                      <  1:177537/111‑1 (MQ=255)
                                                                                                                  tttAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCaga                                                      >  2:177537/1‑111 (MQ=255)
                                                                                                                  tttAATTCTTAAATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATACACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGaa                           <  3:157766/138‑1 (MQ=255)
                                                                                                                    tAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          <  6:141817/105‑1 (MQ=255)
                                                                                                                    tAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          >  5:141817/1‑105 (MQ=255)
                                                                                                                    tAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGga                              <  3:80663/133‑1 (MQ=255)
                                                                                                                    tAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGga                              >  4:80663/1‑133 (MQ=255)
                                                                                                                      aTTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCata                      <  8:284628/139‑1 (MQ=255)
                                                                                                                       ttCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATAtt                                                                >  5:213179/1‑96 (MQ=255)
                                                                                                                       ttCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATAtt                                                                <  6:213179/96‑1 (MQ=255)
                                                                                                                            aCATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          >  8:13114/1‑97 (MQ=255)
                                                                                                                            aCATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCt                                                          <  7:13114/97‑1 (MQ=255)
                                                                                                                            aCATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTcc                  <  8:176220/137‑1 (MQ=255)
                                                                                                                            aCATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCAt                >  3:317148/1‑139 (MQ=255)
                                                                                                                              atatTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTg              <  6:95501/139‑1 (MQ=255)
                                                                                                                              atatTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTg              <  5:37934/139‑1 (MQ=255)
                                                                                                                               tatTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCAAATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTGc             <  8:334857/139‑1 (MQ=255)
                                                                                                                                      tCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTGCTTGTTca      >  3:237800/1‑139 (MQ=255)
                                                                                                                                          tGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTGCTTGTTCAcagt  >  2:118187/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGCCAATACGAAGTTGAAATTTCACTTCTTAAAAAGCTTAATAAGGAATACCAATTAAATTAAACAATCTTGTTGGTAACAAACATCCTCTTACTTATTGTAAGAGGATGTTTGTTTAATTCTTACATATTTTATCTTTGCAATCTGGCTTACGAGTTGCATCCACAGTAAATGATCTTACTTCGTCTTTAGTTTTGTCATAAATTCCCCATATTACCCCTCAGAGAAGGATAGGGGGATTATTTTGGAGAATCATATTCCATTGCTTGTTCACAGT  >  CP006881/1845807‑1846083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑