Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,177,974 C→A 5.2% V8L (GTA→TTA)  U712_10895 ← Dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,177,9740CA 5.2% 240.7 / 3.4 77V8L (GTA→TTA) U712_10895Dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (35/38);  new base A (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

ACAGACATCACATCAGATAATTTTTGCTGGTCGTATGTATGGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAAGAGATATCAA  >  CP006881/2177841‑2178101
                                                                                                                                     |                                                                                                                               
aCAGACATCACATCAGATAATTTTTGCTGGTCGTATGTATGGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTaa                                                                                                                            >  4:268912/1‑139 (MQ=255)
                                   tgtatgGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTAC‑TTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTcc                                                                                        <  8:145833/139‑1 (MQ=255)
                                     tatgGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTcc                                                                                        <  2:3156/138‑1 (MQ=255)
                                       tgGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCCCGCGGGCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttc                                                                                     >  6:188142/1‑139 (MQ=255)
                                        ggTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttct                                                                                    >  4:117860/1‑139 (MQ=255)
                                          tCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttcttc                                                                                  >  2:298508/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGCCTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttcttctt                                                                                >  8:256529/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGTTCTCCTCTACTTTCcttcttctt                                                                                >  3:186236/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTcc                                                                                        <  8:157861/131‑1 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTcc                                                                                        >  7:157861/1‑131 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttcttctt                                                                                >  3:110626/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCcttcttctt                                                                                >  2:23020/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTGTCcttcttgtt                                                                                >  5:48602/1‑139 (MQ=255)
                                            tATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCGGTCAATTTAACAGCGTCTTGCCCCATTCTACCACGCGGTCCGGCAATGACTACTTTAAGTGTTACGTGTGACAGCTTGATCTCCACTCCTTTCctgcttctt                                                                                >  8:156458/1‑139 (MQ=255)
                                                  cccGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCGTCCACGCGGTCCGGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCATTCTTCTTTTCTgg                                                                          >  7:196575/1‑139 (MQ=255)
                                                   ccGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTATGGt                                                                         >  7:182568/1‑139 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATctcct                                                                                                 >  2:109356/1‑114 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATctcct                                                                                                 <  1:109356/114‑1 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTc                                                                        >  6:357245/1‑139 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTc                                                                        >  5:63450/1‑139 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATATTGATCTCCACTACTTTCCTTCTTCTTTTCTGGTc                                                                        >  7:209352/1‑139 (MQ=255)
                                                    cGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCAACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACATTCCAACTTCATTTATGGTc                                                                        >  6:80685/1‑139 (MQ=255)
                                                      aCAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTc                                                                        >  5:199698/1‑137 (MQ=255)
                                                       cAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAg                                                                     <  2:370177/139‑1 (MQ=255)
                                                       cAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAg                                                                     <  1:23020/139‑1 (MQ=255)
                                                        aaGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGc                                                                    <  3:240604/139‑1 (MQ=255)
                                                         aGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCGACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCg                                                                   <  5:80685/139‑1 (MQ=255)
                                                         aGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCg                                                                   <  1:91303/139‑1 (MQ=255)
                                                           gTCAAAATGTGGTGTTCGTTCTGCCAATTTAACAGCTTCCTGCCCCATTCTTCCACGCGGTCCCGCAATGACTAATTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGAt                                                                 >  7:115930/1‑139 (MQ=255)
                                                           gTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGAt                                                                 >  6:78320/1‑139 (MQ=255)
                                                           gTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGAt                                                                 >  5:263685/1‑139 (MQ=255)
                                                           gTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGAt                                                                 >  5:218205/1‑139 (MQ=255)
                                                                    aGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGTTCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTAtctc                                                        <  4:186236/139‑1 (MQ=255)
                                                                     ggTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGAtctc                                                                                                   <  2:169416/95‑1 (MQ=255)
                                                                     ggTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGAtctc                                                                                                   >  1:169416/1‑95 (MQ=255)
                                                                      gtgtTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGAGATCTTGATCTCGTCTACTTTCGTTCTTCTTTTCTGGTCCAGCGATCTTTATATCTa                                                      >  4:184046/1‑139 (MQ=255)
                                                                      gtgtTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTg                                                                                                        <  8:95683/89‑1 (MQ=255)
                                                                      gtgtTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTg                                                                                                        >  7:95683/1‑89 (MQ=255)
                                                                      gtgtTCGTTCTGCCAATTTAACAGCTTCCTGCCCCATTCATCCACGCGGTCCCGCAATGACTAATTTAATTGTTTCGTTTTACATCTTGAAATCCTCTACTTTACTTCATCTTTTATGGTCCAGCGATCTTTATatctt                                                      >  1:282117/1‑138 (MQ=255)
                                                                         ttcgttcTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTa                                                   >  1:204413/1‑139 (MQ=255)
                                                                           cgttcTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCg                                                                   >  5:195484/1‑121 (MQ=255)
                                                                           cgttcTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCg                                                                   <  6:195484/121‑1 (MQ=255)
                                                                           cgttcTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGGCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTAtt                                                 <  6:11449/139‑1 (MQ=255)
                                                                                tGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCATGAAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTAGTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTGTAGTATTAAAtt                                            <  8:196575/139‑1 (MQ=255)
                                                                                 gTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAAtt                                            >  1:288967/1‑138 (MQ=255)
                                                                                      tttAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCa                                      <  2:288967/139‑1 (MQ=255)
                                                                                           cAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGaac                                 <  3:184046/139‑3 (MQ=255)
                                                                                           cAGCTTCTGGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGAcc                                 <  7:156458/139‑1 (MQ=255)
                                                                                              cttcttGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGAATGACCCgg                              >  6:220822/1‑139 (MQ=255)
                                                                                                tcttGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTc                            >  2:70184/1‑139 (MQ=255)
                                                                                                      cccctccttcCACGCGCTCCTGCCATGACAACCATAATTGTCTCGCGTGACCTCTTGATCTCCTCTACTCTCCTTCATCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGGGGGc                      <  2:266176/133‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      <  5:241504/139‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      <  2:234455/139‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      <  6:218205/139‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      <  5:220822/139‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGGCGTGGGc                      <  6:63450/139‑1 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATATCTAGTATTAAATTTATGCATGACCCGGTCGttgtc                      <  8:159432/139‑5 (MQ=255)
                                                                                                      cccATTCTTCCACGCGGTCCCGCAATGACTAATTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTTGTATTAAATTTATGCATGACTCGGTCGTGAGc                      <  8:115930/139‑1 (MQ=255)
                                                                                                          ttcttcCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTAtt                                                 >  3:159067/1‑108 (MQ=255)
                                                                                                          ttcttcCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTAtt                                                 <  4:159067/108‑1 (MQ=255)
                                                                                                          ttcttcCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGAATGACCCGGTCGTgggggtag                  >  8:67790/1‑134 (MQ=255)
                                                                                                                cACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTaaa            <  8:182568/139‑1 (MQ=255)
                                                                                                                cACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTaaa            <  1:70184/139‑1 (MQ=255)
                                                                                                                cACGCGGTACCGCAATGACTAATTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTTGTATTAAATTTATGCATGACTCGGTCGTGAGCTTCCTCTaaa            <  2:282117/139‑1 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTAtctc                                                        <  6:316951/93‑1 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTAtctc                                                        >  5:316951/1‑93 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAaga          <  5:357245/139‑1 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAaga          <  4:110626/139‑1 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAaga          <  3:268912/139‑1 (MQ=255)
                                                                                                                  cgcgGTCCTGCAATGACTACTTTAATTGTATCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAaga          <  2:197048/139‑1 (MQ=255)
                                                                                                                    cgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAAt                                             <  3:191568/102‑1 (MQ=255)
                                                                                                                    cgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAAt                                             >  4:191568/1‑102 (MQ=255)
                                                                                                                    cgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAAgaga        <  2:204413/139‑1 (MQ=255)
                                                                                                                    cgGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGGCGTGGGCTTCCTCTAAAgaga        <  6:199698/139‑1 (MQ=255)
                                                                                                                       tCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTa                                                >  5:166096/1‑96 (MQ=255)
                                                                                                                       tCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTa                                                <  6:166096/96‑1 (MQ=255)
                                                                                                                            cAATGACTACTTTATTTGTTTAGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAAGAGATATCaa  <  3:163759/137‑1 (MQ=255)
                                                                                                                                 actactTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      >  4:38483/1‑112 (MQ=255)
                                                                                                                                 actactTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGc                      <  3:38483/112‑1 (MQ=255)
                                                                                                                                     |                                                                                                                               
ACAGACATCACATCAGATAATTTTTGCTGGTCGTATGTATGGTCTATGGCCCCGACAAGGTCAAAATGAGGTGTTCGTTCTGTCAATTTAACAGCTTCTTGCCCCATTCTTCCACGCGGTCCTGCAATGACTACTTTAATTGTTTCGTTTGACATCTTGATCTCCTCTACTTTCCTTCTTCTTTTCTGGTCCAGCGATCTTTATCTCTAGTATTAAATTTATGCATGACCCGGTCGTGGGCTTCCTCTAAAGAGATATCAA  >  CP006881/2177841‑2178101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑