Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,305,499 A→T 8.3% V237E (GTG→GAG)  U712_11655 ← Putative propionyl‑CoA carboxylase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,305,4990AT 8.3% 139.3 / 4.1 54V237E (GTG→GAG) U712_11655Putative propionyl‑CoA carboxylase beta chain
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

AATCACCTTCCGAACATCATAAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGCCGGTAA  >  CP006881/2305363‑2305637
                                                                                                                                        |                                                                                                                                          
aaTCACCTTCCGAACATCATCAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATACCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCAcc                                                                                                                                          <  2:70337/139‑1 (MQ=255)
 aTCACCTTCCGAACATCATAAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCAccc                                                                                                                                         <  7:96539/139‑1 (MQ=255)
    aCCTTCCGAACATCATAAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTg                                                                                                                                      >  3:135089/1‑139 (MQ=255)
         cccaaCATCATAAGCCTTTGTCGTATCTGCAGGGACATGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCa                                                                                                                                   <  2:180797/134‑1 (MQ=255)
          cgataatcatAAGGCTTTGTCGTATCAGCAGGTACAAGAGGGTTTAATAACGTTCTGGACTCCTCTTTCTCTGGCTTCGGCTATGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGa                                                                                                                                 <  7:281381/133‑1 (MQ=255)
          cGAACATCATAAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTTACCCCTGTCAGa                                                                                                                                 <  1:320097/138‑1 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACTGTTTAATAACGGTCTGGACGACTCTTTCTCTGGCTTCGGCTCTGTTGTCAGCACATTCAGAGTCAAATAATACAGTATTTTTCTCAACCCTGTCAGAACCACAATCTCCGTATga                                                                                                               >  5:185236/1‑138 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCTCCCCTGTCAGAACCTCCTTCTCCGTATGc                                                                                                               >  3:140085/1‑139 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCTCCCCTGTCAGAACCTCCTTCTCCGTATGc                                                                                                               >  3:145555/1‑139 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGc                                                                                                               >  2:14943/1‑139 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGc                                                                                                               >  4:41286/1‑139 (MQ=255)
                           tGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCGCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGACCCTCCTTCTCCGTATGc                                                                                                               >  4:174012/1‑139 (MQ=255)
                             tCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGccc                                                                                                             <  6:103390/139‑1 (MQ=255)
                                  tCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCGCCCCTGTCAGAACCTCCTTCTCCGTATGACCGGaa                                                                                                         >  4:294683/1‑138 (MQ=255)
                                                    tttAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGACCCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGa                                                                                      >  7:56863/1‑139 (MQ=255)
                                                    tttAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGa                                                                                      <  3:174012/139‑1 (MQ=255)
                                                    tttAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGa                                                                                      >  4:227332/1‑139 (MQ=255)
                                                       aataaCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACg                                                                                    <  1:225739/138‑1 (MQ=255)
                                                         taaCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCTCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 <  4:140085/139‑1 (MQ=255)
                                                         taaCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCTCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 <  4:145555/139‑1 (MQ=255)
                                                         taaCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 <  5:140764/139‑1 (MQ=255)
                                                         taaCGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 <  1:14943/139‑1 (MQ=255)
                                                              gTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATg                                                                            >  5:203408/1‑139 (MQ=255)
                                                              gTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATg                                                                            >  5:331796/1‑139 (MQ=255)
                                                              gTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATg                                                                            >  7:372949/1‑139 (MQ=255)
                                                                      gCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCg                                                                    <  3:227332/139‑1 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCAccc                                                                                                                                         >  6:26217/1‑68 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCAccc                                                                                                                                         <  5:26217/68‑1 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGa                                                                                                                                 <  7:104578/76‑1 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGa                                                                                                                                 >  8:104578/1‑76 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGa                                                                                                                                 >  8:202789/1‑76 (MQ=255)
                                                                        ctctTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGa                                                                                                                                 <  7:202789/76‑1 (MQ=255)
                                                                        ctctTTCTCTGGCGTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCAGTCAGAACCTCCTTCGCCGTATGACCGGAAAAGTGGGCGTGTCCGCTGACTGCATTATGTATACCCCCt                                                                  >  2:202447/1‑139 (MQ=255)
                                                                                  ggCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 >  7:100766/1‑114 (MQ=255)
                                                                                  ggCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCa                                                                                 <  8:100766/114‑1 (MQ=255)
                                                                                  ggCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTc                                                                 <  7:43630/130‑1 (MQ=255)
                                                                                  ggCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCACGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTc                                                                 >  8:43630/1‑130 (MQ=255)
                                                                                     ttCCGCTCCGTTGTCCGGCCATTCAGCGTCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGctct                                                     <  6:366952/139‑1 (MQ=255)
                                                                                       cGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGctct                                                     <  4:135089/137‑1 (MQ=255)
                                                                                       cGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGctct                                                     <  8:372949/137‑1 (MQ=255)
                                                                                                    cGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGAATCCCCGCTCCGCCTACGCTCTCCGCATCCCCCTGtt                                      >  2:267103/1‑139 (MQ=255)
                                                                                                       ccATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCGGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTccc                                   >  7:364287/1‑139 (MQ=255)
                                                                                                       ccATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTccc                                   >  6:141841/1‑139 (MQ=255)
                                                                                                       ccATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTccc                                   >  6:335533/1‑139 (MQ=255)
                                                                                                       ccATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTccc                                   >  4:129544/1‑139 (MQ=255)
                                                                                                                ggCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACtt                          <  6:116185/139‑1 (MQ=255)
                                                                                                                  cAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCGCCGCCTAAGCTCTCCGCATCGACCTGTGCCCCTGTCACtttt                        >  8:94315/1‑139 (MQ=255)
                                                                                                                        aGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAAt                   <  1:202447/138‑1 (MQ=255)
                                                                                                                        aGACAGTAGATTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAAt                   <  3:41286/138‑1 (MQ=255)
                                                                                                                              tagtTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTc            >  3:45703/1‑139 (MQ=255)
                                                                                                                              tagtTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTc            >  2:63068/1‑139 (MQ=255)
                                                                                                                                   ttCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGc        <  6:331796/138‑1 (MQ=255)
                                                                                                                                   ttCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGc        <  6:203408/138‑1 (MQ=255)
                                                                                                                                   ttCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGc        <  1:63068/138‑1 (MQ=255)
                                                                                                                                        aCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGc        <  6:78502/133‑1 (MQ=255)
                                                                                                                                        aCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGc        >  5:78502/1‑133 (MQ=255)
                                                                                                                                        aCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGCCGGtaa  >  7:337615/1‑139 (MQ=255)
                                                                                                                                        aCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGCCGGtaa  >  6:270096/1‑139 (MQ=255)
                                                                                                                                        aCCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGCCGGtaa  >  8:272403/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AATCACCTTCCGAACATCATAAGGCTTTGTCGTATCAGCAGGGACAAGACGGTTTAATAACGGTCTGGACGCCTCTTTCTCTGGCTTCGGCTCTGTTGTCCGGCCATTCAGCGGCAAATAAGACAGTAGTTTTCTCACCCCTGTCAGAACCTCCTTCTCCGTATGCCCGGAAAAGTGGGCGTTTCCGCTGACGGCATTATGTATCCCCGCTCCGCCTAAGCTCTCCGCATCAACCTGTTCCCCTGTCACTTTTTCAATCACCTTCGGGCCGGTAA  >  CP006881/2305363‑2305637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑