Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,339,741 A→G 8.0% M138T (ATG→ACG)  U712_11830 ← DNA repair protein recN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,339,7410AG 8.0% 143.3 / 3.3 50M138T (ATG→ACG) U712_11830DNA repair protein recN
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCTTTCAGAAAGCTGCTTAAGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATCCCTTCTCATGACG  >  CP006881/2339605‑2339878
                                                                                                                                        |                                                                                                                                         
ttCGCTTTCAGAAAGCTGCTTAAGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCAt                                                                                                                                          >  5:318277/1‑138 (MQ=255)
   gCTTTCAGAAAGCTGCTTAAGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTaa                                                                                                                                       <  2:100774/138‑1 (MQ=255)
                 gctTAAGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTGTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGGCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGtg                                                                                                                        <  8:110402/139‑1 (MQ=255)
                      aGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGcc                                                                                                                   <  7:169678/139‑1 (MQ=255)
                           tttGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTg                                                                                                               <  6:318277/138‑1 (MQ=255)
                            ttGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTACCAGCTGGTTATCGTGCTGCCCGTGaa                                                                                                             >  5:205614/1‑139 (MQ=255)
                                gcagcTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATc                                                                                                         <  7:205927/139‑1 (MQ=255)
                                gcagcTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATc                                                                                                         <  7:211921/139‑1 (MQ=255)
                                   gcTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCtaa                                                                                                      >  4:78984/1‑139 (MQ=255)
                                    cTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCtaa                                                                                                      >  4:370985/1‑138 (MQ=255)
                                          aTACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGc                                                                                               <  7:238427/139‑1 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCCCTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATATTCGTCTTCCGTTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  4:306843/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTGTTCCGTTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  2:184556/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAatat                                                                                                          >  5:288755/1‑124 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAatat                                                                                                          <  6:288755/124‑1 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  7:219154/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  6:281978/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  3:65546/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  2:336657/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  8:299577/1‑139 (MQ=255)
                                              cGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGc                                                                                           >  1:280018/1‑139 (MQ=255)
                                               gCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGGGAATATCTAATAAAAGCCTGcc                                                                                          >  8:239970/1‑139 (MQ=255)
                                                cTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCg                                                                                         <  8:259654/139‑1 (MQ=255)
                                                   gTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGAtc                                                                                      >  8:326302/1‑139 (MQ=255)
                                                   gTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGAt                                                                                       <  2:38699/138‑1 (MQ=255)
                                                   gTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGAt                                                                                       <  1:336657/138‑1 (MQ=255)
                                                   gTACCCGTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGAGGATTTTCGTCTTCCATATACAGCTGGGTATCGTGCTGCCCGTGAATATCTATTATACGCCTGCCGAtc                                                                                      >  4:314760/1‑139 (MQ=255)
                                                        cTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGc                                                                                                                                   <  8:46111/89‑1 (MQ=255)
                                                        cTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGc                                                                                                                                   >  7:46111/1‑89 (MQ=255)
                                                           ccTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGtg                                                                                                                        >  3:278858/1‑97 (MQ=255)
                                                           ccTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGtg                                                                                                                        <  4:278858/97‑1 (MQ=255)
                                                              gATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCCGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTg                                                                            >  6:10774/1‑138 (MQ=255)
                                                                  cGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCa                                                                       <  7:299577/139‑1 (MQ=255)
                                                                   gTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCaa                                                                      <  8:219154/139‑1 (MQ=255)
                                                                    ttttGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAAt                                                                     <  2:280018/139‑1 (MQ=255)
                                                                     tttGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATc                                                                    >  3:230935/1‑139 (MQ=255)
                                                                                                ggCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCa                                          >  4:261186/1‑138 (MQ=255)
                                                                                                 gCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCaaa                                        <  3:78984/139‑1 (MQ=255)
                                                                                                 gCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCaaa                                        >  2:207261/1‑139 (MQ=255)
                                                                                                   aaaCTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACg                                      <  7:326302/139‑1 (MQ=255)
                                                                                                         gTCCAAAAGCTGGAGATGATTTTCGTCTTCCGTTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCtttt                                 <  1:184556/138‑1 (MQ=255)
                                                                                                         gTCCAAAAGCTGGAGATGATTTTCGTCTTCCGTTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCtttt                                 <  3:306843/138‑1 (MQ=255)
                                                                                                                     gagaTGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCt                     >  5:289121/1‑138 (MQ=255)
                                                                                                                           atTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATcc              <  6:286457/139‑1 (MQ=255)
                                                                                                                            ttttCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATccc             <  3:316640/139‑1 (MQ=255)
                                                                                                                            ttttCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATccc             <  5:281978/139‑1 (MQ=255)
                                                                                                                               tCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGc                            <  6:188041/121‑1 (MQ=255)
                                                                                                                               tCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATAACTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGc                            >  5:188041/1‑121 (MQ=255)
                                                                                                                                      ccATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGCCAAACGCTTTTCCCGCTCGTTCTGATATCCCTACTCATCAc   >  2:34898/1‑139 (MQ=255)
                                                                                                                                       cATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATCCCTTCTCATGACg  <  3:314760/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTCGCTTTCAGAAAGCTGCTTAAGCTTTTTGAGCAGCTTCACATACCGCTGGTACCCTTCCTGATACGTTTTGAGCGCGCTTTCCACTTCGGCCCCGGCAAACTTGTCCAAAAGCTGGAGATGATTTTCGTCTTCCATTAACAGCTGGTTATCGTGCTGCCCGTGAATATCTAATAAAAGCCTGCCGATCTCTCTTAGTGAAGCAATCGTGACAAGCTTGCCATTGACACGGCAAACGCTTTTCCCGCTCGTGCTGATATCCCTTCTCATGACG  >  CP006881/2339605‑2339878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑