Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,411,528	A→G	5.4%	N162N (AAT→AAC)	U712_12280 ←	putative endonuclease 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,411,528	0	A	G	5.4%	228.5 / 3.6	74	N162N (AAT→AAC)	U712_12280	putative endonuclease 4
Reads supporting (aligned to +/- strand):  ref base A (45/25);  new base G (2/2);  total (47/27)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TCATTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCAA  >  CP006881/2411391‑2411661                                                                                                                                          |                                                                                                                                      tCATTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCa                                                                                                                                       <  6:274440/138‑1 (MQ=255) tCATTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCa                                                                                                                                       <  2:80005/138‑1 (MQ=255) tCATTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAt                                                                                                                                      >  5:274440/1‑139 (MQ=255) tCACTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCa                                                                                                                                       <  5:124605/138‑1 (MQ=255)   aTTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     <  6:181771/138‑1 (MQ=255)   aTTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     <  8:369658/138‑1 (MQ=255)             aGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     >  1:267663/1‑128 (MQ=255)             aGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     <  2:267663/128‑1 (MQ=255)             aGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGc                                                                                                                          >  8:64211/1‑139 (MQ=255)             aGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGc                                                                                                                          >  3:296455/1‑139 (MQ=255)             aGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGc                                                                                                                          <  6:73141/139‑1 (MQ=255)                      aTACGGGCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGGCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATaa                                                                                                                 <  6:81606/139‑1 (MQ=255)                            tCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTgcg                                                                                                           >  3:259407/1‑139 (MQ=255)                            tCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTgcg                                                                                                           >  4:357220/1‑139 (MQ=255)                                tGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAg                                                                                                       <  6:24584/139‑1 (MQ=255)                                tGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAg                                                                                                       <  7:64211/139‑1 (MQ=255)                                      tGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGctcct                                                                                                  >  6:213314/1‑138 (MQ=255)                                             aTCGAACTCATTTAGCACTCCGTCAAAGGCTTCGACAATGTTATACCCGGCATCATGTGTAAGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCtt                                                                                          <  6:33049/139‑1 (MQ=255)                                                  aCTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGGCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGcc                                                                                     <  4:120186/139‑1 (MQ=255)                                                   cTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCg                                                                                    <  3:357220/139‑1 (MQ=255)                                                               tCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTATGCCGCATTCTGATCCt                                                                        >  1:90236/1‑139 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     >  5:82577/1‑77 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCAtt                                                                                                                                     <  6:82577/77‑1 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtt                                                                       >  1:56540/1‑139 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtt                                                                       >  4:118495/1‑139 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtt                                                                       >  4:328686/1‑139 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtt                                                                       >  1:212791/1‑139 (MQ=255)                                                                ccGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtt                                                                       >  1:134109/1‑139 (MQ=255)                                                                 cGTCAAAGTCTTCGACAATGTTATACCCGGCATCATTTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCATTCGATAATCTGCGAAAGATCCTCAACTATTCTGCCGCATAGTGATACta                                                                       >  1:129689/1‑137 (MQ=255)                                                                 cGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtta                                                                      >  2:143812/1‑138 (MQ=255)                                                                  gccAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCttt                                                                      >  6:311090/3‑138 (MQ=255)                                                                  gTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCACTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCtttt                                                                     >  4:315560/1‑139 (MQ=255)                                                                             cGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGt                                                          >  7:97032/1‑139 (MQ=255)                                                                                   tGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAACGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTaa                                                    >  1:311594/1‑139 (MQ=255)                                                                                     ttATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCGCAAAGCTTCTGCCGCATTCTGCTCCTTTTCCAGCCATTGTTTCTAATg                                                  >  2:341017/1‑139 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCGTTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  2:21339/1‑138 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCGTTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCACAAAGCTTCTGCCGCAGACTGATCCTTTTCCAGCCATTGTATCTAATCCAATCtt                                           >  6:252777/1‑137 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTgg                                          >  1:104968/1‑139 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  5:293266/1‑138 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  1:110866/1‑138 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  3:359906/1‑138 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  2:38876/1‑138 (MQ=255)                                                                                             ggCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTg                                           >  7:37536/1‑138 (MQ=255)                                                                                              gCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGa                                         <  3:328686/139‑1 (MQ=255)                                                                                               catcatGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGa                                         >  4:61902/1‑138 (MQ=255)                                                                                               catcatGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGa                                         >  3:270600/1‑138 (MQ=255)                                                                                               catcatGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGa                                         >  5:280666/1‑138 (MQ=255)                                                                                               catcatGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAAGCTGGa                                         >  2:133782/1‑138 (MQ=255)                                                                                                   atGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACAtt                                     <  4:359906/138‑1 (MQ=255)                                                                                                       gtATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGAt                                >  7:127102/1‑139 (MQ=255)                                                                                                        tATGGCACGTGTCATAGCACACTGACAGCTGCTCGTTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTACTCAAATCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGGAATCTGGACATTTTGAt                                <  5:252777/138‑1 (MQ=255)                                                                                                        tATGGCACGTGTCAAAGCACACTGACAGCTGCTCGTTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGAt                                <  1:21339/138‑1 (MQ=255)                                                                                                         aTGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTc                              >  3:369685/1‑139 (MQ=255)                                                                                                           ggCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCg                             >  7:33293/1‑138 (MQ=255)                                                                                                           ggCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCg                             >  8:256976/1‑138 (MQ=255)                                                                                                            gCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCGAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGa                           <  2:201234/139‑1 (MQ=255)                                                                                                             cACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGat                          >  1:60559/1‑139 (MQ=255)                                                                                                                gtgtCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTTCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaa                       <  5:213314/139‑1 (MQ=255)                                                                                                                 tgtCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaat                      <  8:97032/139‑1 (MQ=255)                                                                                                                 tgtCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaat                      <  5:311090/139‑1 (MQ=255)                                                                                                                 tgtCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaat                      <  4:259407/139‑1 (MQ=255)                                                                                                                 tgtCAAAGCACACTGACAGCTGCTCACTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaat                      <  3:315560/139‑1 (MQ=255)                                                                                                                  gtCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGatcaat                      >  5:102793/1‑138 (MQ=255)                                                                                                                     aaaGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCAc                   <  2:60559/138‑1 (MQ=255)                                                                                                                     aaaGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCAc                   <  1:203789/138‑1 (MQ=255)                                                                                                                     aaaGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACt                  <  4:296455/139‑1 (MQ=255)                                                                                                                       aGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACtt                 >  1:127514/1‑138 (MQ=255)                                                                                                                       aGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACtt                 >  3:162833/1‑138 (MQ=255)                                                                                                                            acTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCAtt                                                            <  2:365964/90‑1 (MQ=255)                                                                                                                            acTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCAtt                                                            >  1:365964/1‑90 (MQ=255)                                                                                                                            acTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCAttt            >  6:159302/1‑138 (MQ=255)                                                                                                                                aCAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAATCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCTGATCAATCACTTCATTTAAGcc       >  7:55524/1‑139 (MQ=255)                                                                                                                                      gctCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCaa  >  8:186767/1‑138 (MQ=255)                                                                                                                                      gctCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCaa  >  1:353664/1‑138 (MQ=255)                                                                                                                                      gctCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCaa  >  1:206758/1‑138 (MQ=255)                                                                                                                                       ctCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCaa  <  2:110866/137‑1 (MQ=255)                                                                                                                                       ctCATTGTGTGTAACGCCGTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCaa  <  6:293266/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TCATTGATATGAAGCACTTTAATACGGTCAATGCCAATGATTTTATCGAACTCATTTAGCACTCCGTCAAAGTCTTCGACAATGTTATACCCGGCATCATGTGTATGGCACGTGTCAAAGCACACTGACAGCTGCTCATTGTGTGTAACGCCTTCGATAATCTGCGCAAGCTCCTCAAAGCTTCTGCCGCATTCTGATCCTTTTCCAGCCATTGTTTCTAATGCAATCTGGACATTTTGATTCGGATCAATCACTTCATTTAAGCCTTCAA  >  CP006881/2411391‑2411661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑