Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,627,471 G→A 5.5% C304C (TGC→TGT)  U712_13530 ← tRNA‑specific 2‑thiouridylase mnmA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,627,4710GA 5.5% 240.3 / 2.9 72C304C (TGC→TGT) U712_13530tRNA‑specific 2‑thiouridylase mnmA
Reads supporting (aligned to +/- strand):  ref base G (31/37);  new base A (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GCCGTCATAGAAGACAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCAAGATCTTTGCCTAC  >  CP006881/2627336‑2627608
                                                                                                                                       |                                                                                                                                         
gCCGTCATAGAAGACAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAg                                                                                                                                        >  1:32051/1‑139 (MQ=255)
   gTCATAGAAGACAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGaa                                                                                                                                     <  8:253229/139‑1 (MQ=255)
            gaCAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCg                                                                                                                            <  4:343370/139‑1 (MQ=255)
             aCAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGc                                                                                                                           <  7:373181/139‑1 (MQ=255)
              cAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGcc                                                                                                                          <  6:363776/139‑1 (MQ=255)
               aaCGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTGCACCTTCGCCCGTCAGGCGCACTGTTACTTTATGATCCTCGTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGccc                                                                                                                         >  8:162225/1‑139 (MQ=255)
                 cGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTACAGGAAATCTCTTCg                                                                                                                            <  4:302277/134‑1 (MQ=255)
                 cGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTACAGGAAATCTCTTCg                                                                                                                            <  4:297156/134‑1 (MQ=255)
                 cGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTACAGGAAATCTCTTCg                                                                                                                            >  3:302277/1‑134 (MQ=255)
                 cGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTACAGGAAATCTCTTCg                                                                                                                            >  3:297156/1‑134 (MQ=255)
                     ttGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATg                                                                                                                   <  1:302503/139‑1 (MQ=255)
                         cccGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCAt                                                                                                                    <  1:139471/134‑1 (MQ=255)
                         cccGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCAt                                                                                                                    >  2:139471/1‑134 (MQ=255)
                          ccGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGGCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTTGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATc                                                                                                              <  3:346042/139‑1 (MQ=255)
                          ccGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATc                                                                                                              <  1:131810/139‑1 (MQ=255)
                             gCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCg                                                                                                            >  6:193155/1‑138 (MQ=255)
                             gCGGTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGa                                                                                                           <  6:202149/139‑1 (MQ=255)
                               gTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGa                                                                                                           <  3:231461/137‑1 (MQ=255)
                               gTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGa                                                                                                           >  4:231461/1‑137 (MQ=255)
                                  aCAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTa                                                                                                      >  1:2559/1‑139 (MQ=255)
                                   cAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGACCGTa                                                                                                      <  6:18433/138‑1 (MQ=255)
                                    atggcgGACTTGTTCATCAAAGATGACTTCCTCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACTGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTAcc                                                                                                    <  3:19900/136‑1 (MQ=255)
                                    aGCGCTGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTAcc                                                                                                    <  5:134500/139‑1 (MQ=255)
                                    aGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTAcc                                                                                                    <  1:125338/139‑1 (MQ=255)
                                    aGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTAcc                                                                                                    <  2:32051/139‑1 (MQ=255)
                                    aGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTAcc                                                                                                    <  5:157896/139‑1 (MQ=255)
                                        cgGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCa                                                                                                                     <  7:1821/118‑1 (MQ=255)
                                        cgGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCa                                                                                                                     >  8:1821/1‑118 (MQ=255)
                                         ggACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCt                                                                                               <  8:231049/139‑1 (MQ=255)
                                                ttCATCAAAGATGACTTCCGCTTCCCCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATAttt                                                                                        >  4:91207/1‑139 (MQ=255)
                                                ttCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATAttt                                                                                        >  7:234774/1‑139 (MQ=255)
                                                   atcaAAGTTGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGCTCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGATCGTACCCAGCTGATATttgt                                                                                      <  8:61416/138‑1 (MQ=255)
                                                     caAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGc                                                                                   >  2:132202/1‑139 (MQ=255)
                                                                  cGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCg                                                                      <  1:68312/139‑1 (MQ=255)
                                                                             cGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGt                                                                        <  8:109769/126‑1 (MQ=255)
                                                                             cGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGt                                                                        >  7:109769/1‑126 (MQ=255)
                                                                             cGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATaaaa                                                            <  5:309526/138‑1 (MQ=255)
                                                                             cGCCAGTCATGAGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGACCTTCATGATATCCGAACGTACCCAGATGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAg                                                           >  2:288209/1‑139 (MQ=255)
                                                                              gCCAGTCATGCGCCCTGTTACTTTATGATACTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATAGTTGTTGCTGTGATTTTGTCTGAATATaaaatc                                                          >  6:328818/1‑137 (MQ=255)
                                                                                cAGTCATGCGCACTG‑TACTTTAAGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCggg                                                       <  1:164098/139‑1 (MQ=255)
                                                                                   tCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGtt                                                     >  5:238757/1‑139 (MQ=255)
                                                                                        cgcACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGa                                                >  4:48307/1‑139 (MQ=255)
                                                                                             tGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCt                                           >  4:266862/1‑139 (MQ=255)
                                                                                               ttACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAAccc                                            <  4:80183/136‑1 (MQ=255)
                                                                                               ttACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAAccc                                            >  3:80183/1‑136 (MQ=255)
                                                                                                  cTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTTATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATc                                      <  5:193155/139‑1 (MQ=255)
                                                                                                  cTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGAt                                       <  2:201285/138‑1 (MQ=255)
                                                                                                  cTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGAt                                       <  3:266862/138‑1 (MQ=255)
                                                                                                   tttATGATCCTCTTTGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCt                                     <  4:217996/139‑1 (MQ=255)
                                                                                                     tATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTa                                                                                                      >  4:328621/1‑72 (MQ=255)
                                                                                                     tATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTa                                                                                                      <  3:328621/72‑1 (MQ=255)
                                                                                                     tATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGAt                                       >  8:130894/1‑135 (MQ=255)
                                                                                                     tATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGAt                                       <  7:130894/135‑1 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCTGAATATAAAAGCGGGTTATGGAACCATTGATCTACg                                  >  3:155693/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCTGGTTATGGAACCCTTGATCTACg                                  >  5:249702/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATgg                                                 >  5:203424/1‑123 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATgg                                                 <  6:203424/123‑1 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  6:46095/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  4:8618/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  5:24024/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  1:268452/1‑138 (MQ=255)
                                                                                                       tGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTAGCCCTTCATGATATAAGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  5:265332/1‑138 (MQ=255)
                                                                                                         aTCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCgg                                                                     <  3:83791/101‑1 (MQ=255)
                                                                                                         aTCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCgg                                                                     >  4:83791/1‑101 (MQ=255)
                                                                                                         aTCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACg                                  >  7:71227/1‑136 (MQ=255)
                                                                                                                        aCGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGTGTTATGGAACCCTTGAt                                       >  6:181169/1‑116 (MQ=255)
                                                                                                                        aCGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGAt                                       <  5:181169/116‑1 (MQ=255)
                                                                                                                        aCGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCa                <  8:114916/139‑1 (MQ=255)
                                                                                                                         cGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCaa               <  3:354405/139‑1 (MQ=255)
                                                                                                                         cGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCCTGATCTACGTAGAGGATGTTCTTTTCaa               <  6:164062/139‑1 (MQ=255)
                                                                                                                                  gCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCAAGATCTTTGc      <  5:46095/139‑1 (MQ=255)
                                                                                                                                      tGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCAAGATCTTTGCCTAc  >  3:224129/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                         
GCCGTCATAGAAGACAACGGCTTGTCCCGGCGTTACAGCGCGGACTTGTTCATCAAAGATGACTTCCGCTTCACCTTCGCCAGTCATGCGCACTGTTACTTTATGATCCTCTTGGCGGTAACGGAATTTAGCCGTGCAGGAAATCTCTTCGCCCTTCATGATATCCGAACGTACCCAGCTGATATTTGTTGCTGTGATTTTGTCGGAATATAAAAGCGGGTTATGGAACCCTTGATCTACGTAGAGGATGTTCTTTTCAAGATCTTTGCCTAC  >  CP006881/2627336‑2627608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑