Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,632,433 T→A 7.8% K17N (AAA→AAT)  U712_13555 ← Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,632,4330TA 7.8% 170.5 / 3.3 60K17N (AAA→AAT) U712_13555Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base T (26/29);  new base A (3/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATCACAGACGATTTT  >  CP006881/2632302‑2632571
                                                                                                                                   |                                                                                                                                          
aTGGCTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGa                                                                                                                                     <  5:91464/139‑1 (MQ=255)
 tGGCTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGa                                                                                                                                     >  4:188461/1‑138 (MQ=255)
  ggCTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTG‑AAGTCCTCTTTACCGaag                                                                                                                                   <  1:48837/138‑2 (MQ=255)
    cTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCGCTCAGCAGGTGGGTGCCCTCTTTTTAATCTTGTAAGTCCTCATTACCGAATg                                                                                                                                  >  8:339697/1‑138 (MQ=255)
      gATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCGCAGCAGGTGGGTGCCCTCTTTTAAAGCGTGTAAGTCCTCTTTACCGAATGaa                                                                                                                                >  1:363509/1‑138 (MQ=255)
       aTCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGaaaa                                                                                                                              <  6:213678/139‑1 (MQ=255)
          cATGAGCAGCTGTTTTTTCTGCTCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAaga                                                                                                                            <  3:188461/138‑1 (MQ=255)
          cATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAaga                                                                                                                            <  2:311390/138‑1 (MQ=255)
                   cTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACg                                                                                                                  <  6:97958/139‑1 (MQ=255)
                   cTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACg                                                                                                                  >  1:68633/1‑139 (MQ=255)
                                             aGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCGCAAAt                                                                                        <  3:51668/139‑1 (MQ=255)
                                                  cAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCaaa                                                                                    >  5:100097/1‑138 (MQ=255)
                                                  cAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCaaa                                                                                    >  4:253621/1‑138 (MQ=255)
                                                        tGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGt                                                                             >  1:116391/1‑139 (MQ=255)
                                                          ccGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGtt                                                                            <  1:220951/138‑1 (MQ=255)
                                                          ccGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGtt                                                                            <  7:201945/138‑1 (MQ=255)
                                                             ttGCATCTCTACTTTGTGTTGAACCCTCTCTCATCAGGTGGGTGCACTATTTTAAATCTTGTAAGTCCTCTTTACCGAATTAAAAGAGGCATGTACGCCATTTAAAAATGCTGGCTCCCAAATCAAAAAAATGTTCGGt                                                                        <  8:285650/139‑1 (MQ=255)
                                                                      tCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 <  1:108171/137‑1 (MQ=255)
                                                                      tCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 <  3:51331/137‑1 (MQ=255)
                                                                      tCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 <  2:304095/137‑1 (MQ=255)
                                                                           tGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGgtgt                                                           <  8:219659/138‑1 (MQ=255)
                                                                           tGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGgtgt                                                           <  2:68633/138‑1 (MQ=255)
                                                                           tGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGgtg                                                            <  6:289297/137‑1 (MQ=255)
                                                                                  aaCCCGCTCTCAGCAGGTTGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTaa                                                   <  7:339697/139‑1 (MQ=255)
                                                                                       gCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACAt                                              <  8:254246/139‑1 (MQ=255)
                                                                                       gCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACAt                                              <  1:203552/139‑1 (MQ=255)
                                                                                       gCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACAt                                              <  2:116391/139‑1 (MQ=255)
                                                                                       gCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACAt                                              >  3:57906/1‑139 (MQ=255)
                                                                                             agcagGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         <  3:253621/138‑1 (MQ=255)
                                                                                             agcagGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         <  1:142489/138‑1 (MQ=255)
                                                                                                       tgCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAAc                              >  5:7590/1‑139 (MQ=255)
                                                                                                       tgCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAAc                              >  3:67051/1‑139 (MQ=255)
                                                                                                           ctctTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGt                          <  1:153822/139‑1 (MQ=255)
                                                                                                             ctTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATTTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTc                         >  1:354275/1‑138 (MQ=255)
                                                                                                              ttttAAATCTTGTAAGTCCTCATTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 >  4:275778/1‑97 (MQ=255)
                                                                                                              ttttAAATCTTGTAAGTCCTCATTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 >  4:270743/1‑97 (MQ=255)
                                                                                                              ttttAAATCTTGTAAGTCCTCATTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 <  3:270743/97‑1 (MQ=255)
                                                                                                              ttttAAATCTTGTAAGTCCTCATTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACt                                                                 <  3:275778/97‑1 (MQ=255)
                                                                                                                   aaTCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACg                                                                                                                  <  4:46808/43‑1 (MQ=255)
                                                                                                                   aaTCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACg                                                                                                                  >  3:46808/1‑43 (MQ=255)
                                                                                                                     tCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCata                >  2:18860/1‑139 (MQ=255)
                                                                                                                     tCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCata                >  6:316504/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACTCCATTTAAAAATCCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAAAACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  5:296371/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAATACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  2:29207/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAg                                                               <  8:261745/89‑1 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAg                                                               >  7:261745/1‑89 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         <  4:255101/111‑1 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         >  3:104598/1‑111 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         <  4:104598/111‑1 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGa                                         >  3:255101/1‑111 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATc                   <  2:343989/133‑1 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATc                   >  1:343989/1‑133 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  3:24891/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  7:9795/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  7:346675/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  5:277718/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  3:272447/1‑139 (MQ=255)
                                                                                                                        tGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATc              >  7:68122/1‑138 (MQ=255)
                                                                                                                         gTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATca             >  5:4473/1‑138 (MQ=255)
                                                                                                                            aGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATCACAGa         <  6:209288/139‑1 (MQ=255)
                                                                                                                                   tttACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATCACAGACgatttt  >  3:275290/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                          
ATGGCTGATCCATGAGCAGCTGTTTTTTCTGCGCACAAAAAATAAAGTCCCAATAGTGCCGTTGCAGCTTTCCTTTGTTTTGAACCCGCTCTCAGCAGGTGGGTGCCCTCTTTTAAATCTTGTAAGTCCTCTTTACCGAATGAAAAGAGGCATGTACGCCATTTAAAAATGCGGGCTCCCAAATCAAAAAAATGTTCGGTCAAAACTAGGTGTACAACTAACACATCAGGACTTTATTTAACTTGTCAAAATCATATCACAGACGATTTT  >  CP006881/2632302‑2632571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑