| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,801,996 | G→A | 100% | A327T (GCC→ACC) | U712_14440 → | UPF0118 membrane protein ytvI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,801,996 | 0 | G | A | 99.9% | 120.2 / ‑7.7 | 42 | A327T (GCC→ACC) | U712_14440 | UPF0118 membrane protein ytvI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/24); minor base C (1/0); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGC > CP006881/2801886‑2802125 | gacagaTCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 3:22209/134‑1 (MQ=255)aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCATCTGACAGAGCCAAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 4:209031/139‑1 (MQ=255)aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 1:340265/139‑1 (MQ=255) ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt < 5:331946/139‑1 (MQ=255) tATCCTGTACCTCGTCGTGAGTATTTAGCCGGGGGTGACAGAGCCCCACATACTAAGTAAAACTAAGGGGTACGATCCGCTCGGCGCACTCCTCTCCCATTTTTCCGGCGTTAAATTAATACGCGTTTTAGGAcctgag > 4:22209/1‑134 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg < 3:117018/137‑1 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg < 2:186548/137‑1 (MQ=255) tACCTCGTCGGTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGAGCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGGTTTTAGGACTGATTGCggg < 1:252578/137‑1 (MQ=255) cgtcgtTCTTATTCAGCGGCAGCTGCCAGCGCCCAAGATACTAAGTAAATCTATCGGGATCGCTCCGCAGGCAACCCGCATAACCCTGTTTGCCCGCTGTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACAGGcg > 6:185906/1‑138 (MQ=255) tcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGt > 5:68732/1‑139 (MQ=255) tcgtTCTTATTCAGCGGCAGCTGACAGACCCCAAGAAACTAATTAAATCTATAGGGATCGATCCGCTGGCAACCCTCATACCCCAGTTTGCCGGCTTTTAATTATTAGGGTTTTTATGACTGATTGCGGGACCGGCTg > 4:117018/1‑138 (MQ=255) tcgtTATTATTAAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATAGATCCGCTGGCCACACTCCTCACACTGTTTGCCTGCGTTAAATTTTTAGGGTTTTTAGGCAGTATTGGGGGACCGGCTg > 2:89872/1‑138 (MQ=255) gtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTAc < 2:146827/139‑1 (MQ=255) gtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTAc < 4:55364/139‑1 (MQ=255) aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 1:356835/138‑1 (MQ=255) gACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTAAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCttt > 4:12966/1‑138 (MQ=255) cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc > 8:155114/1‑62 (MQ=255) cccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 7:155114/62‑1 (MQ=255) aTACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGgcg < 2:307561/139‑1 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGTGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCacaac > 5:206861/1‑130 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGTGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCacaac < 6:206861/130‑1 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt > 1:298250/1‑119 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt < 2:298250/119‑1 (MQ=255) aaGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGCCCGGCTGTACTCGTCCTCATCCACGCTTTCATCCCAACAGGCGCAtt > 8:339813/1‑138 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 1:69408/139‑1 (MQ=255) aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg > 4:175170/1‑138 (MQ=255) aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg > 2:158962/1‑138 (MQ=255) aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg < 6:68732/138‑1 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa > 4:324825/1‑138 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg > 5:205655/1‑58 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 6:205655/58‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 1:134938/95‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc > 2:134938/1‑95 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 3:3123/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata > 6:183191/1‑137 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 5:183191/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 3:12966/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 1:90982/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg > 1:243483/1‑139 (MQ=255) acacTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAGAAGAAATATGGTCCTATATCACCGTTCAGc < 1:89872/139‑1 (MQ=255) cATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa > 2:280984/1‑104 (MQ=255) cATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 1:280984/104‑1 (MQ=255) | AAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGC > CP006881/2801886‑2802125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |