| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 278,241 | A→T | 20.1% | T299S (ACG→TCG) | U712_01300 → | putative transporter ycbK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 278,241 | 0 | A | T | 20.1% | 98.3 / 4.1 | 52 | T299S (ACG→TCG) | U712_01300 | putative transporter ycbK |
| Reads supporting (aligned to +/- strand): ref base A (13/28); new base T (9/2); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACGAATCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGCA > CP006881/278103‑278374 | aaaCGAATCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTa < 6:178308/139‑1 (MQ=255)aaaCGAAACTGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTa < 4:13691/139‑1 (MQ=255) ctCGGGGCACAGCGATCCGGCGCCATTTGAATGGCAGGGCCTATCGTGACGATTTTCTATGGAGGTTTATTTCTGGGTGAACGGCTCGGGCTCATTCAAGTCTTTTGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGct < 4:5710/138‑2 (MQ=255) ctCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 4:363545/138‑1 (MQ=255) agcgcggCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 8:176566/134‑1 (MQ=255) agcGGGGCCCAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGGGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 4:204859/137‑1 (MQ=255) aTCGGGGCACAGCGCGCCGCCGCCATTTAAATGGCAGGGCCTAGCCGGCCGATTGACTATGGTGCTTGATTTCTCGGTGAACGGCTCGGGCTCATTCAAGGCATTGGATGCGGTGGC‑TGCTTTTTTTTCA‑GTACggcc > 7:279588/1‑136 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTTGATGCGGTGGC‑GGTTTTTTTTTGA‑TTTCGGGc > 1:122602/1‑138 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTTTTTTTTTTCA‑TTAACGGCa > 2:49632/1‑139 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACgggga > 5:134374/1‑137 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 7:211100/139‑1 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 1:29176/139‑1 (MQ=255) aTCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCa < 5:205413/139‑1 (MQ=255) tggCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGACCGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGa < 7:315790/138‑1 (MQ=255) cgccATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGTCATGGAATACAGGAAG‑CTGaa > 8:37349/1‑138 (MQ=255) ccTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTTTTTTTTTTAA‑TTTACGGCAAGGAATAAAAGATA‑GTGAAAGAAGGAaaaaaaaaaaaaa > 8:266018/1‑138 (MQ=255) tCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGACTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGTTTTTTTTTGA‑TTTAGGGGCGGGTATACAGGAAG‑GTGAAAACAGGAAAAAAAGAATAAatttt > 7:320157/1‑139 (MQ=255) aCGATTTTCTATGGAGCTTTATTTCTCGGTTAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGTAAAAAAAGAATAATTTTtata < 8:319893/137‑1 (MQ=255) aCGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTTTTTTTTTTTATTTAGGGGCTTGG‑ATAAAGGAAG‑CTGGAAAACTGAAAAAAAGAATATTTTTtatta > 2:47464/1‑136 (MQ=255) aCGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGTTTTTTTTTGATTTATGGGC‑TGGTAAAAATGAAG‑CTGAAAACAGGAAAAAAAGAAAAATTTTTATaa > 5:111385/1‑138 (MQ=255) aCGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGTTTTTTTTTGA‑TTTAGGGGCTGGATTAAAGGCAG‑CTGAAAACACGTAAAAAATAATAATTTTTTTaa > 7:303433/1‑138 (MQ=255) aCGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGTTTTTTTTTCA‑TTTCGGGCATGGAATACAGGAAG‑CTGCAAACAGGAAAAAAAGAATAATTTTTATaa > 7:31342/1‑138 (MQ=255) tttCTATGGAGATTTATTTCTCGGAGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGTC‑GTATGTTTTTTAA‑ATAAGTGCATTGATTAAAGGAAG‑CTAAAAAAACATAAAAAAAAATAATTTTTTTAAAacaa > 6:97849/1‑137 (MQ=255) tCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGTAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAacacac‑a > 8:261959/1‑139 (MQ=255) cTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTTTTTTTTTTTA‑TTTAGTGGGTTGAATAAAAGTAGAATTAAAAAAGAAAAAAAATATAAATTTTTTTAAAAAAAAC‑AAGttttt > 6:231250/1‑138 (MQ=255) cTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTAA‑TTAAGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTc > 5:334673/1‑138 (MQ=255) cTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGATTTTTTTTTA‑TTTAGGGGAATGCATAAAGGAAG‑CGGGAAAAAGGAAAAAAAAAATAATTATTATAAAAAACAA‑ACGgtttta > 1:346714/1‑137 (MQ=255) tATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAAt < 1:311365/138‑1 (MQ=255) ctcGGTGAACGTCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAAt < 4:277884/139‑1 (MQ=255) cGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGCGGTTTTTTTTTGAA‑TTTCGGGTATGG‑ATAAAGGAAA‑AGGTAAAAAATAAAAAAAAAATAAATTTTATAAAAAAAACAACGTTTTTAAAtaaaaaa > 4:8586/1‑132 (MQ=255) cGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTTTAA‑TTTCCGGCAATGAAAAAACGAAG‑CGGGAAACAAGAAAAAAACAAAAAATTTTATAAAACCCAC‑ACAGGTTTCAATTaaaaaa > 6:197974/1‑136 (MQ=255) cGGTGAACGGCTCGGGCTCATTCAAGGCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATaa < 5:197974/139‑1 (MQ=255) ggTGAACGGCTCGGGCTCATTCAAGTCATTTGATGCGGTGGC‑GTATTTTTTGTCA‑TTACTGGCATTGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAAt < 7:266018/136‑1 (MQ=255) ggTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAAt < 2:122602/136‑1 (MQ=255) ggTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAAt < 5:231250/136‑1 (MQ=255) ggTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATaa < 1:22217/138‑1 (MQ=255) aaCGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GGTTTTTTTTTTT‑TTAAGGGGAGTGTATAAAGGAAG‑CGGAAAACAGGAAAAAAAAAAAAATTTTTTTAAAAAAAAA‑ACCTTTTTGAATTAAAATaatttt > 8:321014/1‑134 (MQ=255) aCGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTaa < 7:56085/139‑1 (MQ=255) ttCAAGTCATTGGATGCGGTGGC‑GTTTTTTTTGTCA‑TTAACGGCAATGTAAAAAGGAAG‑CTGAAAACAGGTAAAAAACAATAATTTTTATAAAAAACAC‑ACCTTTTTAAATTACAATAAGTTTAATTTCGGGGGAGttt > 8:314920/1‑139 (MQ=255) aaGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGttttt < 3:112631/138‑1 (MQ=255) gTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGa < 3:51619/139‑1 (MQ=255) tCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑ATACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGAt < 3:93439/139‑1 (MQ=255) ttGGATGCGGTGGC‑GTTTTTTTTTTGT‑TTAAGGGGCTGGGTTAAAGGGAG‑CGGAAAACAGGAAAAAAAGAAAAATTTTTTTAAAAAAAAC‑ACCGTTTTCAATTTCAATAATTGTAATTTAGTGTGTGtttttttttttt > 1:102550/1‑139 (MQ=255) ttGGATGCGGTGGC‑GTATTTTTTTTAA‑TTAAGGGCAATGAAAAAAAGAAA‑CAGAAAAAAAGAAAAAAAAAAAAATTTTTATAAAAAAAAA‑ACCTTTTTAAAATAAAAAAAATTTAATTAAATGTTTGTTTTTTTAtttt > 4:164959/1‑139 (MQ=255) gATGCGGTGGC‑GTATTTTTTGTCA‑TTTCGGGCATGGTATACAGGAAG‑CTTAAAACAGGAAAAAATGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATTAGTGTAATTACGTGTGTGTTTTTTGATTTTGTa < 7:213689/139‑1 (MQ=255) gATGCGGTGGC‑GTATTTTTTGTCA‑TTTCGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTAGGTGTGTGTTTTTTGATTTTGtt < 8:114342/139‑2 (MQ=255) gATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTa < 7:321014/139‑1 (MQ=255) gATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTa < 7:117675/139‑1 (MQ=255) ggTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGt < 8:303433/139‑1 (MQ=255) gTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAATAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGtt < 6:134374/139‑1 (MQ=255) tCA‑TTACGGTCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGCa < 7:37349/139‑1 (MQ=255) tCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACATGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGc < 2:346714/138‑1 (MQ=255) tCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGc < 7:115898/138‑1 (MQ=255) tCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGCa < 7:326396/139‑1 (MQ=255) | AAACGAATCGGGGCACAGCGAGCCGCCGCCATTTCAATGGCAGGGCCTATCCTGACGATTTTCTATGGAGCTTTATTTCTCGGTGAACGGCTCGGGCTCATTCAAGTCATTGGATGCGGTGGC‑GTATTTTTTGTCA‑TTACGGGCATGGAATACAGGAAG‑CTGAAAACAGGAAAAAAAGAATAATTTTTATAAAACACAC‑ACGTTTTTCAATTACAATAAGTGTAATTACGTGTGTGTTTTTTGATTTTGTAAAGGTTAGGTGAGGAGATGCA > CP006881/278103‑278374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |