Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,210,982 C→T 5.2% S310S (AGC→AGT)  U712_16525 → Spore germination protein A2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,210,9820CT 5.2% 261.8 / 2.9 77S310S (AGC→AGT) U712_16525Spore germination protein A2
Reads supporting (aligned to +/- strand):  ref base C (40/33);  new base T (2/2);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TATTTATTGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCTTTTTCATTG  >  CP006881/3210852‑3211118
                                                                                                                                  |                                                                                                                                        
tatttattGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGAt                                                                                                                                  >  1:132488/1‑139 (MQ=255)
    tattGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTAt                                                                                                                               <  6:216491/138‑1 (MQ=255)
       tGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCgg                                                                                                                            >  4:363058/1‑138 (MQ=255)
       tGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCgg                                                                                                                            >  6:363871/1‑138 (MQ=255)
       tGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCgg                                                                                                                            >  1:322643/1‑138 (MQ=255)
       tGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATACAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCgg                                                                                                                            >  4:215644/1‑138 (MQ=255)
           cGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCa                                                                                                                                         <  5:295189/121‑1 (MQ=255)
           cGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCa                                                                                                                                         >  6:295189/1‑121 (MQ=255)
           cGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATaa                                                                                                                       <  2:353115/139‑1 (MQ=255)
           cGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATaa                                                                                                                       >  2:39231/1‑139 (MQ=255)
              tttGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTAt                                                                                                                               <  7:148814/128‑1 (MQ=255)
              tttGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTAt                                                                                                                               >  8:148814/1‑128 (MQ=255)
                       tttttACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCAAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGACTTTTAtt                                                                                                           >  6:344993/1‑139 (MQ=255)
                        ttttAGTGGTGGTCTGGATTAACCAGATTTTCGCCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGTTTGGTGATTATCGGCATAACGGTCTTTTAttt                                                                                                          >  7:207522/1‑139 (MQ=255)
                        ttttACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTAttt                                                                                                          >  5:322494/1‑139 (MQ=255)
                        ttttACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGATAAAGAAGACATTTGGATTATCGACTAAAACAATTATGGTGATTATCGGCATAACTGTCTTTTAttt                                                                                                          >  7:202195/1‑139 (MQ=255)
                        ttttACTGGTGGTCTGGATTATCCAGTTTTTCACAACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTAttt                                                                                                          >  2:257199/1‑139 (MQ=255)
                                gtggtCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAATACAAGCTTGGTGATTATCGGCATAACGGTCTTTTATTTTTctctct                                                                                                  >  7:275544/1‑139 (MQ=255)
                                gtggtCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTctctct                                                                                                  >  5:356750/1‑139 (MQ=255)
                                  ggtCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTgg                                                                                                <  4:267848/139‑1 (MQ=255)
                                        gATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGa                                                                                           <  3:363058/138‑1 (MQ=255)
                                           tATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGCCATTTGGATTATAGACTAAAACAATCATGGTGATTATAGGCATAACGGTCTTTTATTTTTATATCTGTCCGGATGAc                                                                                       >  3:105694/1‑139 (MQ=255)
                                             tCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGc                                                                                     >  2:8333/1‑139 (MQ=255)
                                             tCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGc                                                                                     >  6:326667/1‑139 (MQ=255)
                                             tCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATAGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTATCTGGCCGTATGACGc                                                                                     >  5:193367/1‑139 (MQ=255)
                                                aGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCaa                                                                                  <  4:104076/139‑1 (MQ=255)
                                                  tttttCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATACCGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAAt                                                                                 >  5:138477/1‑138 (MQ=255)
                                                  tttttCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAAt                                                                                 >  1:128584/1‑138 (MQ=255)
                                                  tttttCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAAt                                                                                 >  1:269639/1‑138 (MQ=255)
                                                      tCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAg                                                                             <  8:143743/138‑1 (MQ=255)
                                                       caccacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGt                                                                            <  5:10403/138‑1 (MQ=255)
                                                       caccacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGt                                                                            >  3:57056/1‑138 (MQ=255)
                                                        accacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTc                                                                           <  5:326667/138‑1 (MQ=255)
                                                        accacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTc                                                                           <  5:363871/138‑1 (MQ=255)
                                                        accacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTc                                                                           <  8:193874/138‑1 (MQ=255)
                                                        accacATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCa                                                                          <  4:305702/139‑1 (MQ=255)
                                                          ctcaTTTGTGATTTACGGATACTTTGCCGCTATCGGGGTAAAGATGACATTTGGATTTTCGACTAAAACAAGTATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCatg                                                                        <  8:207522/137‑1 (MQ=255)
                                                          cacaTTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCatg                                                                        <  8:268153/139‑1 (MQ=255)
                                                          cacaTTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCatg                                                                        <  2:132488/139‑1 (MQ=255)
                                                          caatttttTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGTATTTTCTAATAAAACAAGTATGGTGATTATCGGCATAACGGTGTTTTATTTTTCTCTCTGGCCGGATGACGCCAAtcatgtcatg                                                                        <  8:202195/135‑1 (MQ=255)
                                                                tGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGta                                                                   <  6:356750/138‑1 (MQ=255)
                                                                tGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGta                                                                   <  7:316373/138‑1 (MQ=255)
                                                                tGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGta                                                                   <  1:39231/138‑1 (MQ=255)
                                                                    aTTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATACCGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATTGc                                                               >  2:361051/1‑138 (MQ=255)
                                                                    aTTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCg                                                              <  7:4177/139‑1 (MQ=255)
                                                                      ttACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGa                                                                                           <  2:362112/108‑1 (MQ=255)
                                                                      ttACGGATACTTTGACGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGa                                                                                           >  1:362112/1‑108 (MQ=255)
                                                                       tACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGAt                                                            >  4:218245/1‑138 (MQ=255)
                                                                           gATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCt                                                       <  3:215644/139‑1 (MQ=255)
                                                                            aTACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGCCGCCAATCAAGTCATGATGTATAGCGATTATCtt                                                      >  6:324626/1‑139 (MQ=255)
                                                                            aTACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAg                                                                             <  7:201218/116‑1 (MQ=255)
                                                                            aTACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAg                                                                             >  8:201218/1‑116 (MQ=255)
                                                                            aTACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGTGATTATCtt                                                      >  8:17006/1‑139 (MQ=255)
                                                                            aTACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCtt                                                      >  8:330431/1‑139 (MQ=255)
                                                                                  tGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCta                                                 >  2:17307/1‑138 (MQ=255)
                                                                                    ccGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGGCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCCAGTCATGATGTATAGCGATTATCTTGGCTatat                                              <  2:46620/139‑1 (MQ=255)
                                                                                          aCGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGCCGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGt                                        >  5:163692/1‑139 (MQ=255)
                                                                                          aCGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGt                                        <  1:257199/139‑1 (MQ=255)
                                                                                              gCTAAAGAAGACATTTGGATTATCGACTAAACCCAGCATGGTGAGTCTCGGCATAACGGGCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTcc                                    <  1:143486/139‑1 (MQ=255)
                                                                                              gCTAAAGAAGACATTTGGATTATAGACTACAACAAGCATGTTGATTATCGGCATAACGGTCTTGTATTTATCTCTCTGGCCGGATGACGCCAAACGATGCAAGATGTATAGCGATAATGGTGGCTCTATTTTTggtggc                                    >  2:185035/1‑134 (MQ=255)
                                                                                                      agaCATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATc                                                                                >  1:129170/1‑87 (MQ=255)
                                                                                                      agaCATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATc                                                                                <  2:129170/87‑1 (MQ=255)
                                                                                                       gaCATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTg                           >  8:103968/1‑139 (MQ=255)
                                                                                                        aCATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGc                          >  2:326883/1‑139 (MQ=255)
                                                                                                           tttGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTc                       <  2:97690/139‑1 (MQ=255)
                                                                                                                 ttATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCa                 <  6:138477/139‑1 (MQ=255)
                                                                                                                 ttATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCa                 <  2:269639/139‑1 (MQ=255)
                                                                                                                    tctaCTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATtc              >  3:18732/4‑139 (MQ=255)
                                                                                                                    tCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCAtt               <  3:218245/138‑1 (MQ=255)
                                                                                                                    tCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCAt                >  3:214307/1‑137 (MQ=255)
                                                                                                                    tCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATtc              >  3:169401/1‑139 (MQ=255)
                                                                                                                    tCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATtc              >  6:73009/1‑139 (MQ=255)
                                                                                                                    tCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATtc              >  1:100190/1‑139 (MQ=255)
                                                                                                                        cTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCtt          <  4:169401/139‑1 (MQ=255)
                                                                                                                         tAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCttt         >  8:133265/1‑139 (MQ=255)
                                                                                                                           aaaCAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCttt         <  6:322494/137‑1 (MQ=255)
                                                                                                                                aGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCTTTTTCATTg  <  4:18732/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                        
TATTTATTGAACGGTTTGAATCCTTTTTACTGGTGGTCTGGATTATCCAGTTTTTCACCACATTTGTCATTTACGGATACTTTGCCGCTAACGGGCTAAAGAAGACATTTGGATTATCGACTAAAACAAGCATGGTGATTATCGGCATAACGGTCTTTTATTTTTCTCTCTGGCCGGATGACGCCAATCAAGTCATGATGTATAGCGATTATCTTGGCTATATTTTTGTCTCCCTGTTTCTGCTTCCGTTCATTCTCTTTTTCATTG  >  CP006881/3210852‑3211118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑