Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,529,094	C→T	5.8%	G35D (GGC→GAC)	U712_18080 ←	Acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,529,094	0	C	T	5.8%	230.7 / 2.6	69	G35D (GGC→GAC)	U712_18080	Acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CGACTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACTCCTTATTATGCATTT  >  CP006881/3528957‑3529219                                                                                                                                          |                                                                                                                              cGACTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGcc                                                                                                                              <  4:280626/139‑1 (MQ=255) cGACTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGcc                                                                                                                              <  3:192885/139‑1 (MQ=255) cGACTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAAATTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGcc                                                                                                                              <  5:4722/139‑1 (MQ=255)    cTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCaaa                                                                                                                           >  1:65951/1‑139 (MQ=255)    cTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCaaa                                                                                                                           >  2:102325/1‑139 (MQ=255)    cTCACGGTTTTCCAGTTAAACGGCCGACTGCTTGGGAAATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCaa                                                                                                                            >  8:229037/1‑138 (MQ=255)       ccGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATAc                                                                                                                        <  6:343994/139‑1 (MQ=255)       ccGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATAc                                                                                                                        <  4:314195/139‑1 (MQ=255)          gTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCTGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGTCAAATACAtg                                                                                                                     >  6:325064/1‑139 (MQ=255)               ccAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGTCAAATACAtgtgtg                                                                                                                 <  5:325064/138‑1 (MQ=255)               ccAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACAtgtgtg                                                                                                                 <  6:232054/138‑1 (MQ=255)                 atttAAGCGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGGCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAACTACATGAGGGaca                                                                                                              <  6:276087/137‑1 (MQ=255)                 aGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGaca                                                                                                              <  7:138133/139‑1 (MQ=255)                      aaCGGCCGTCTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTTCCGGGCAAAGATAATTTCAGTTACTTTATCTTGTAAAGAGTCAAATACCGCATCAATTTTTGCACCTGTAATGCCAAATACATGTGTGACACCttt                                                                                                         <  6:158264/139‑2 (MQ=255)                      aaCGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         <  3:153669/139‑1 (MQ=255)                      aaCGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         <  7:52456/139‑1 (MQ=255)                      aaCGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         <  4:231408/139‑1 (MQ=255)                      aaCGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         <  3:327496/139‑1 (MQ=255)                                     gggCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAAt                                                                                           >  1:170269/1‑138 (MQ=255)                                     gggCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAAt                                                                                           >  7:296482/1‑138 (MQ=255)                                      ggCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATca                                                                                         <  7:368051/139‑1 (MQ=255)                                          aTGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATcaacaa                                                                                     >  5:342697/1‑139 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         >  4:146627/1‑112 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTg                                                                                                         <  3:146627/112‑1 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATca                                                                                         >  6:357339/1‑128 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATca                                                                                         <  5:357339/128‑1 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTc                                                                              >  8:71207/1‑139 (MQ=255)                                                 ctgcGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTc                                                                              >  8:44779/1‑139 (MQ=255)                                                    cGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGACTGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCg                                                                            <  6:169640/138‑1 (MQ=255)                                                    cGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGc                                                                           >  7:22732/1‑139 (MQ=255)                                                    cGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGc                                                                           >  8:116642/1‑139 (MQ=255)                                                      ttttGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGccc                                                                         <  4:54376/139‑1 (MQ=255)                                                         tGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCct                                                                       <  5:42472/138‑1 (MQ=255)                                                                    ggCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTcaca                                                           <  5:312321/139‑1 (MQ=255)                                                                     gCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTcac                                                            >  1:37906/1‑137 (MQ=255)                                                                       aaCGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAgg                                                        <  1:102325/139‑1 (MQ=255)                                                                               tttCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTg                                                <  4:190553/139‑1 (MQ=255)                                                                                  cAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGtt                                              <  7:229037/138‑1 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCct                                                                       >  2:364100/1‑111 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCct                                                                       <  1:364100/111‑1 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCtt                                           >  7:9876/1‑139 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCtt                                           >  4:35146/1‑139 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCtt                                           >  3:253004/1‑139 (MQ=255)                                                                                    ggTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTGTTCACATGGGATTTTTGTTCtt                                           >  4:10441/1‑139 (MQ=255)                                                                                        cTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTttgt                                       >  3:80401/1‑139 (MQ=255)                                                                                         tttATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTttgtt                                      >  4:75697/1‑139 (MQ=255)                                                                                          ttATCTTGTAACGCGTCAAATACCGCATGAATTTTTGCACCTGGAATGCCAAATACATGTCTGACCCCTTGCTCCACTAAGCAATCAACAGCAAGCTCCGCCCCTCTGATTTTCACTAGGGATTTTTGTTCTTttgtt                                      >  8:353758/1‑138 (MQ=255)                                                                                           tATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTttgttg                                     <  8:22732/138‑1 (MQ=255)                                                                                             tCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCt                                   <  2:37906/138‑1 (MQ=255)                                                                                             tCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCt                                   <  2:170269/138‑1 (MQ=255)                                                                                                 ttAAACCGTAATATACTTCATTAATTTTTGCACCTTGTATGCCAAATACATGTGAGACACTTTGCACCTCTAAGCAATCTACATCAATCTCCGCACCACTGTTTTTCACAAGGTATTTTTGTTCTTTTGTTGCttt                                 <  8:372796/135‑1 (MQ=255)                                                                                                  tAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACAtgtgtg                                                                                                                 <  5:347928/55‑1 (MQ=255)                                                                                                  tAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACAtgtgtg                                                                                                                 >  6:347928/1‑55 (MQ=255)                                                                                                  tAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGt                              >  5:85475/1‑138 (MQ=255)                                                                                                  tAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGt                              >  8:176229/1‑138 (MQ=255)                                                                                                  tAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGAGTTTTGTTCTTTTGTTGCTTTTGt                              >  4:315399/1‑138 (MQ=255)                                                                                                    aaGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCaa                           <  4:253004/139‑1 (MQ=255)                                                                                                    aaGCGTCAAATACCACATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCaa                           <  8:9876/139‑1 (MQ=255)                                                                                                       cGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAacac                        >  4:283283/1‑139 (MQ=255)                                                                                                       cGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAacac                        >  1:253027/1‑139 (MQ=255)                                                                                                         tCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACAccc                      <  5:366038/139‑1 (MQ=255)                                                                                                           aaaTACCGCATCAATTTTTGCACCTGGAATGTCAAATACATGTGTGACACCTTg                                                                                                         >  4:338413/1‑54 (MQ=255)                                                                                                           aaaTACCGCATCAATTTTTGCACCTGGAATGTCAAATACATGTGTGACACCTTg                                                                                                         <  3:338413/54‑1 (MQ=255)                                                                                                              tACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACt                 <  7:176229/139‑1 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGCCACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCCACACCCTCACTCCtta            >  5:286292/1‑139 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCGCTCCtta            >  6:365742/1‑139 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCCCTCCtta            >  8:230151/1‑139 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACt                 >  8:183842/1‑134 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACt                 <  7:183842/134‑1 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACTCCtta            >  8:357031/1‑139 (MQ=255)                                                                                                                   cATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGATTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACTCCtta            >  3:231906/1‑139 (MQ=255)                                                                                                                             tGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACTCCTTATTATGCAttt  >  2:313110/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                              CGACTCCCGGTTTTCCAGTTAAACGGCCGACTGCTTGGGCCATGAATGCTGCGTTTTGTTCGTGCCGGGCAACGATAATTTCAGGTCCTTTATCTTGTAAAGCGTCAAATACCGCATCAATTTTTGCACCTGGAATGCCAAATACATGTGTGACACCTTGCTCCACTAAGCAATCAACAACAAGCTCCGCCCCTCTGTTTTTCACAAGGGATTTTTGTTCTTTTGTTGCTTTTGTCAACACCCTCACTCCTTATTATGCATTT  >  CP006881/3528957‑3529219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑