| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | 100% | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 99.6% | 106.3 / ‑7.1 | 34 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/12); minor base C (1/0); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 100.0% | 90.4 / NA | 34 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/12); total (22/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTA > CP006881/3725124‑3725362 || caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGAtttcttt > 4:36961/1‑139 (MQ=255)caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATACCTCCGCTTCTTTTTGGCAGGCAG‑C‑CATTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGatttattt > 8:27984/1‑139 (MQ=255) aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg > 4:77854/1‑139 (MQ=255) aCCATCCACACCCGAACAGATAAACGCAAACTTTTCAAACAAAACCAGCCCTCCGCTTCTTTTTGGCAGGCAG‑CGT‑TTTT‑A‑GCCGCCCGTTTCCGCCGCCGCCGCCCGAAAGAAACGACGGCCGATTTCTTTTCTCGCa < 7:243976/139‑1 (MQ=255) tCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCAG‑C‑CGTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAACAAAAGACGGACGAATTCTTATCTCGCaa > 3:33650/1‑136 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAACAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGGCCGTTTTCTCCGCCGCAGCCCGCCAGAAAAGACGGCCGATTGCTTTTCTCGCAAACTAGCGGcttgt > 2:268618/1‑136 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCCGCCGCAGCCCGCAAGATAAGACGGCCGAGTTCTTTTCTGGCAACCTAGCGGctctc > 2:138322/1‑138 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTa > 4:330537/1‑139 (MQ=255) aGATACAAGCAATTGTTTCATATAAAAAAATCCCTCCTGTGTTTTTTGTGAGTCAGAG‑T‑TTTT‑C‑GCTGCCTGGTTTCTCCCCCGCCCCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 6:254091/139‑1 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 2:12730/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGCTTTCTTTTCTCGCAACATAGCGGCTCTAGCaaaa > 5:286888/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCTCAAACTAGCGGCTCTAGCaaaa > 8:361302/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAATAGCGGCTCTAGCaaaa > 5:254091/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTATCGCAAACTAGCCGCGCTAGAAAAAc > 1:187479/1‑139 (MQ=255) gCACACGTTTCATACAAAAACATCCCTCCGCTTGTTTTTGGCTGGCAG‑C‑CTTTTT‑C‑GCAGCCGGTTTTCTCCGCCGCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 6:286888/139‑1 (MQ=255) gCAACCGTTGCAAACAACAACATCCCGCCGCTGATTTTGGGCAGGCCG‑C‑CTTTTT‑C‑GCCGCCCGTTTTCTCCGCCGCAGCCCGCAAGAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:72748/139‑1 (MQ=255) caaaccGTTTCATAGAAAAACATCCATCCGCTTCTTTTTGCCAGGAAG‑CAT‑TTTT‑A‑GCACCCCTTTTTCTCAGCCGCCCCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:361302/134‑1 (MQ=255) ccGTTCCATAAAAAACCACCCAGCCGCTTCTTTTGGCCTGCCCG‑C‑CTTTTT‑A‑GCCGCCCGTTTTCCCCGCCGCAGCCCCCAACAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 1:64467/138‑1 (MQ=255) atttttCATATAAAAACATCGCTGCGCTTTTTTTTGCCTGTCCG‑CAT‑TTTT‑A‑GCAGCCGGTTTTCGCAGCCGCACCCCCAAAAAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:69013/136‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGGTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 3:157955/139‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑CAT‑TTTT‑A‑GCAGCCCGTTTTCTAAGCCGCAGCCCGCAAGAAAAGACGGACGAATTATTTTATAGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 5:131165/1‑139 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAtcccg > 8:256491/1‑135 (MQ=255) aCGTTTAATACAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑CAT‑TTTT‑A‑GCAGCCCGTTTTCCCCGCCGCAGCCCGCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 3:330537/139‑1 (MQ=255) aCGTTAAATATAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCG‑C‑C‑TTTTAA‑GCACCCCGTTTTCTCCGCCCCACCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 3:77854/139‑1 (MQ=255) gTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCCCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActct < 8:231783/139‑1 (MQ=255) aaaCATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGAAGGCCGATTTAGTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAATCTATGATAGACCg > 4:220986/1‑139 (MQ=255) cttcttTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTGTCGCAAACTAGCGGCTCGAACAAAACTCATTACTCTATGATATAACGTGGAAAGAGAAtt > 3:33326/1‑139 (MQ=255) cttTTTGGCAGGCAG‑C‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt > 6:178733/1‑139 (MQ=255) ttGGCCGGCAG‑C‑C‑TTTT‑ATCCGGCCCTTTTGCTCAGCCGCAGCCCCCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt < 7:27984/138‑1 (MQ=255) tttt‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGa > 8:110719/1‑138 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa > 8:56151/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 1:42027/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCGTTTGTCGCAAACAACCCGCGCGAGCAAAACTCCTGCCGCTATTATAGACCGTGGAAAGAGAAGTCGTTTGCCCAATATGAGTGTAAAAACCAAc > 1:173964/1‑139 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACAACCGGCTCTAGCAAAACACATTACTCGATGATAGACCGTGGAAAGAGAATTCTTTGTCACAAAATGATTGTAAAAACCAAACAATTTTTa > 2:135032/1‑138 (MQ=255) || CAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTA > CP006881/3725124‑3725362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |