Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,439,2120AC34.9% 44.9 / 11.6 43L90R (CTT→CGT) U712_07375putative MFS‑type transporter ykuC
Reads supporting (aligned to +/- strand):  ref base A (18/10);  new base C (1/14);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 3.06e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCATAATGCTCCTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCAAGGTC  >  CP006881/1439080‑1439346
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gCATAATGCTCCTTCGGCAGAATGGATTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGTAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACg                                                                                                                                   <  3:116102/138‑1 (MQ=255)
    aaTGCTCCTTCGGCAGAATGGCTTGAACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAATATACAAAACACAAGAGTAATAT‑TGCCGGTAAATAACTTCAAAAAAACCACGACAGt                                                                                                                              <  8:70916/139‑1 (MQ=255)
    aaTGCGCATTCGGCAGAATGGCTTGAACCAAACTGTTATCTGCCGGAAAGAAAAACTTTGTAACAGCGATTCTTATAAATAAGATACAAAAAACACCCGTAATAT‑TCCCTGTAAATAAAGTGAAAAAACGCACGACAGt                                                                                                                              <  6:209357/139‑1 (MQ=255)
     aTGCTCCTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACCGCGCTTCTGAAAAATAAGATACAAAAAACAAGATTAATAT‑TGCCGGAAAATAACGTGAAAAAACGCACGACAGt                                                                                                                              <  3:344790/138‑1 (MQ=255)
     aTGCGCATTCGGCTTATTGGCTTGTAACAAAGTTTTTTTTGCTTGAAATAAAAATTTTTTTACCGGCTTTCTTATAAAAAATATAAAAAAAAAATGTTTAATTT‑TGCCGTTAAAAAAAGTTAAAAAAAGCACGACAGt                                                                                                                              <  4:73413/138‑1 (MQ=255)
      tGCTCCTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGt                                                                                                                              >  2:315513/1‑137 (MQ=255)
           cTTCGGCATAATGGCTTGTACCAAACTTTTTTCTGCCTTAAATAAAAAATTTGTTACAGCGATTCTTATAAATAATATAAAAAAAACAAGAGTAATAT‑TGCGGGTAAATAAAGTTAAAAAACGCACGACAGTCAGGc                                                                                                                         <  8:231723/137‑1 (MQ=255)
           cTTCGGCATAATGGCTTGTACCAAACTGTTTTCTGCCGTAAACAAAAACTTTTTTACAGCGCTTATTAAAAATAATATACAAAAACCAAGCGCAATAT‑TGCCGGAAAATAACGTGAAAAAACGCACGACAGTCAGGc                                                                                                                         <  5:271774/137‑1 (MQ=255)
           cTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCTGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCt                                                                                                                       >  1:407560/1‑139 (MQ=255)
           cTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCt                                                                                                                       >  7:231723/1‑139 (MQ=255)
           cTTCGGCAGAATGGCTTGTACAAAACTGTTTTCTGCCGTAAAGAAAAAATTTGTTACAGCGCTTCTGATAAATAAGAAACAAAAAACAAGAGTAATAT‑TGCCGGTAAATAAAGTCAAAAAACCCACGACAGTCAGGc                                                                                                                         <  5:113540/137‑1 (MQ=255)
              cGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGctgtct                                                                                                                    >  3:67892/1‑134 (MQ=255)
                  aGACTGGCTTGTACCAAACTGTTTACAGCCGAAACCAAAAAAGTTGTTACCCCGCTTCTTATAAATAACAACCAAAAAACCAG‑CGCATATGTCCCGTAAAATAACGTCAAAAAAAGCACGACAGTCAGGCCTGCTCTGa                                                                                                                <  4:71279/139‑1 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACCGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  4:473862/1‑139 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  7:24717/1‑139 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  6:28427/1‑139 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  2:392509/1‑139 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  6:104697/1‑139 (MQ=255)
                                  aaCTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTc                                                                                                >  2:275959/1‑139 (MQ=255)
                                       ttttCTGCCGTAAATAAAATCTTTTTTACAGCGCTTATCATAAAAAAGAAAAAAAAAACACTAGTAATAT‑TCCCGGTAAATAATGTTAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGc                                                                                             <  1:315513/137‑1 (MQ=255)
                                       ttttCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAACGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCa                                                                                           >  2:209963/1‑139 (MQ=255)
                                         ttCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTACTGCCAcc                                                                                         >  3:37393/1‑139 (MQ=255)
                                          tCTGCCGTAAATAAAAAATTTGTTACCGCGCTTCTTAAAAATAAGACACAAAAACCAAGAGTAATAT‑TGCGGTAAAATAAAGTGAAAAAACGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCAcc                                                                                         <  7:221682/138‑1 (MQ=255)
                                                        aaaTTTTTTTACACCCTTTCTTATAAAAAATAAAAAAAAAAAAATCCAAACAT‑TCCCGTAAAAAAAAGTAAAAAAACCCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTACTGCCACCTTTTTTCTATCAAAc                                                                          <  4:37393/139‑1 (MQ=255)
                                                           cTTTGTTACCCCGATTCTTATAAATACCAAACAAAAAACAACAGCAAAAT‑TCCCGTAAAAAAACGGCAAAAAACGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCgg                                                                       <  6:7360/139‑1 (MQ=255)
                                                                      cgcTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCgacgac                                                            >  7:446034/1‑139 (MQ=255)
                                                                           cttaaaaataaCAAAAAAAAAACAACCGCAATAT‑TCCCGGAAAATAAAGTCAAAAACCCCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGGCCGCGACGACTccc                                                        <  6:115568/133‑1 (MQ=255)
                                                                              ataaataaGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACg                                                     <  3:186857/138‑1 (MQ=255)
                                                                                  ataaGATAAAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAg                                                <  8:34247/139‑1 (MQ=255)
                                                                                        tACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGAACAGAAATa                                           >  2:243570/1‑138 (MQ=255)
                                                                                                  aaGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCACACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTc                                >  4:357530/1‑139 (MQ=255)
                                                                                                      ggAATAT‑TGCCGGAAAATAAAGTGAAAAAAACCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGaa                            <  4:441641/139‑1 (MQ=255)
                                                                                                         ttat‑GGCCGTTAAATAACTTTAAAAAACCCACGACAGTCAGGCCTGATCTGATCCATTCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAagag                         <  8:24717/138‑1 (MQ=255)
                                                                                                            t‑tGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGaa                       >  7:418315/1‑138 (MQ=255)
                                                                                                            t‑tGCCGGAAAAAAAAGTTAAAAAACGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGGCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGaa                       <  7:101900/138‑1 (MQ=255)
                                                                                                                ccGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACa                   >  4:254663/1‑139 (MQ=255)
                                                                                                                 cGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACAt                  <  1:275959/139‑1 (MQ=255)
                                                                                                                   gTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACAt                  >  1:428359/1‑137 (MQ=255)
                                                                                                                      aataaaGTCAAAAAACGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGc              <  7:9760/138‑1 (MQ=255)
                                                                                                                           actGAAAAAACCCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGGCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGcc        <  5:104697/137‑1 (MQ=255)
                                                                                                                           aGTAAAAAAACCCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGcc        <  3:473862/139‑1 (MQ=255)
                                                                                                                            tttaaaaacaGAACGACAGTAAGGCGTGCTCTGATCAAATCAAAATTTTCTGCAACATTTTTTCTATAAAACCGGGCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCa       <  5:4403/136‑1 (MQ=255)
                                                                                                                            gtcaaaaaaaGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCa       <  1:392509/136‑1 (MQ=255)
                                                                                                                             tGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCaa      <  3:441528/139‑1 (MQ=255)
                                                                                                                             tGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAAATTTCTGCCACCTTTATTCTATCAAACCGGTCCGCGCCGACTCCCCCCATCAGAAATACAAATATGTTCGGAAGAGAATACATCAGCTCCGcc        >  1:442141/1‑137 (MQ=255)
                                                                                                                              aaaaaaaaCCACGACAGTCAGGCCTGCGCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCAAg     <  6:323193/138‑1 (MQ=255)
                                                                                                                                 aaaaaCCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCAAGGTc  <  7:25922/139‑1 (MQ=255)
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GCATAATGCTCCTTCGGCAGAATGGCTTGTACCAAACTGTTTTCTGCCGGAAAGAAAAACTTTGTTACAGCGCTTCTGATAAATAAGATACAAAAAACAAGAGGAATAT‑TGCCGGTAAATAAAGTGAAAAAAAGCACGACAGTCAGGCCTGCTCTGATCCAATCACAATTTTCTGCCACCTTTTTTCTATCAAACCGGTCCGCGACGACTCCCACGATCAGAAATACAAATATGGTCGGAAGAGAATACATCAGCTCCGCCAAGGTC  >  CP006881/1439080‑1439346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑