Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,031	C→T	A46A (GCG→GCA)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,031	0	C	T	100.0%	114.2 / NA	33	A46A (GCG→GCA)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/25);  total (8/25)

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATA  >  CP006881/3724896‑3725152                                                                                                                                        |                                                                                                                          cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCGTTCTGGTTTTCTTGCTAATTACCAGCCATCCGCCATTCATAAAAAGAAACGAGTCCagcg                                                                                                                        >  6:107516/1‑139 (MQ=255) cGGGCCCGCGCTCTCCTGCTTGGGCGATTCCTTGACGGCTTGAAAACATCTTCTTTTATAAAGCGCGGCGGCTTGCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                        <  6:283662/139‑1 (MQ=255)   ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTTCTGCGTTTATTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                      >  1:42360/1‑139 (MQ=255)         ctctctCCTGCTTTGGAGATTCCTGGAATGCTTGCAAACATCTTCTTTTATAATGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACATCAATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAc                                                                                                                <  8:315908/139‑1 (MQ=255)              tcCTGCTTTGGCGCTTCCTTGAAGGCTTGCAAACTTCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                             <  3:22270/137‑1 (MQ=255)                cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                         <  3:357353/139‑2 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCGTTTCTTCCTCCTTAACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCccgcccccggt                                                                                                      >  7:453559/1‑133 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                  <  6:214836/138‑1 (MQ=255)                            ttCCTTGAAGGCTTGCAAACATCCTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTTGTAt                                                                                             <  5:194938/139‑1 (MQ=255)                                  gAAGGCTTGCAAACATCTTCTTTTATAATGCGGGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                        <  5:424822/138‑1 (MQ=255)                                  catggCTTGAAAACATCTTTTTTTATAAAGCGCGCCGGATTTCGCTTTCTGCTTTTATTCCTCATTCACACCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                        <  6:66428/135‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAATGCGCGGCGGCTTGCGCTTTCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                      <  2:162974/139‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                      <  8:275415/139‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                      <  3:268264/139‑1 (MQ=255)                                    aGGCTTGCAAACATCTTATTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                      <  5:420575/138‑1 (MQ=255)                                     ggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa                                                                                    <  4:193701/139‑1 (MQ=255)                                           cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTGCTGCTTTTCTACCGCAATCACAGCCATCCGCCATTCATAACCCGACACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                              <  5:298388/139‑1 (MQ=255)                                                           taccgcgcGGCGGCTTTCCCTTTCGGATTTTCTTCAACATCCACCCCCCACCGCCTTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                              <  2:463066/135‑1 (MQ=255)                                                                cgcgGCGGCTTTCGCTTTCTGCTTTTCTTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTGGTTGGGTTGTTCGCGGCGGCGGATAGGCCCCGGCcgccgccggt                                                         >  5:168702/1‑135 (MQ=255)                                                                   ggcggcTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                      <  2:42360/139‑1 (MQ=255)                                                                        cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                 <  2:24819/139‑1 (MQ=255)                                                                                     cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                       <  6:179587/135‑1 (MQ=255)                                                                                        ttcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGtt                                  >  2:272758/1‑138 (MQ=255)                                                                                             ccTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCCCCGTTTTTGTGTCCGGGGTCACCCCGTtcatca                             >  2:302820/1‑138 (MQ=255)                                                                                                   tCACAGCCATCCGCCATTCATAAAACGAAACAAGTCCTGCGGCCTGCACAGCCGCCGTTGTTTGGTATGTACGCGGAGCCGTATAGACCCCGGCAGCCGCAGGTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                      >  8:203636/1‑139 (MQ=255)                                                                                                    cacaGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                      <  7:210528/138‑1 (MQ=255)                                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt                                                  <  7:252960/105‑1 (MQ=255)                                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt                                                  >  8:252960/1‑105 (MQ=255)                                                                                                         ccATCCGCCATTCAAAAAACCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                <  3:16974/139‑1 (MQ=255)                                                                                                         ccAGCCGCCATTCATTAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGATTCGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGGCACCCAGTTCATCAAAAAGACCATCca                <  8:198209/139‑1 (MQ=255)                                                                                                           aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca              <  8:453559/139‑1 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                 >  7:373901/1‑103 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                 <  8:373901/103‑1 (MQ=255)                                                                                                                 aattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa        <  5:256051/136‑1 (MQ=255)                                                                                                                       tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa  <  5:107516/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                          CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATA  >  CP006881/3724896‑3725152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑