BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,045	T→C	intergenic (‑159/‑142)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,045	0	T	C	99.2%	491.9 / NA	133	intergenic (‑159/‑142)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (51/81); minor base A (1/0); total (52/81)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

AACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAA  >  CP006881/3722909‑3723172

aaCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                 >  3:177135/1‑139 (MQ=255)

 aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                 >  2:354834/1‑138 (MQ=255)

 aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                 >  5:24280/1‑138 (MQ=255)

  cAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGa                                                                                                                               <  3:335030/139‑1 (MQ=255)

  cAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGa                                                                                                                               <  2:136393/139‑1 (MQ=255)

     aaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAg                                                                                                                            >  2:137079/1‑139 (MQ=255)

           ccTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGtttt                                                                                                                      >  6:71411/1‑139 (MQ=255)

               aaaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGt                                                                                                                   >  5:297290/1‑138 (MQ=255)

                aaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCa                                                                                                                 <  6:24280/139‑1 (MQ=255)

                          cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttt‑t                                                                                                     <  4:80316/139‑1 (MQ=255)

                                  ggACACATCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCGACTATTTTTT‑TAGTTaaa                                                                                              <  1:30983/139‑1 (MQ=255)

                                  ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                              <  1:373102/139‑1 (MQ=255)

                                  ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                              <  7:285222/139‑1 (MQ=255)

                                   gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                              >  4:73516/1‑138 (MQ=255)

                                      acaGGGATCCTTTTTCTTTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCAGATTCCCGCTTATTGAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCTAGTATTTTTT‑TTGTTAAATAGt                                                                                          <  5:415238/139‑1 (MQ=255)

                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                          <  4:378078/139‑1 (MQ=255)

                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                          <  1:116289/139‑1 (MQ=255)

                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                          >  5:238160/1‑139 (MQ=255)

                                       caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGtt                                                                                         >  6:239672/1‑139 (MQ=255)

                                        aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                        >  1:103782/1‑139 (MQ=255)

                                        aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                        >  5:49910/1‑139 (MQ=255)

                                          cGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGa                                                                                      >  5:181573/1‑139 (MQ=255)

                                             tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                    >  8:160376/1‑138 (MQ=255)

                                                  tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                              >  2:310263/1‑137 (MQ=255)

                                                  tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                              >  1:41328/1‑137 (MQ=255)

                                                  tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                              >  3:200685/1‑137 (MQ=255)

                                                  tttCATTTACGCAAATTTTAGGTCTTGCATGCTTTACCAGTCACAATCCCGCTTATTAAGATTAAGAATACGCTTTCATCATAAATCATGATAGCTTTTTCTTCAAGA‑ATTTTTATAGTTAAATATTTTTATATAAcat                                                                              >  4:283642/1‑137 (MQ=255)

                                                    tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcatg                                                                             >  3:298664/1‑135 (MQ=255)

                                                                 ttttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCg                                                               <  1:10842/139‑1 (MQ=255)

                                                                    tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                             <  3:119039/138‑1 (MQ=255)

                                                                      ggTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaa                                                           >  7:302149/1‑138 (MQ=255)

                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                    >  3:378499/1‑137 (MQ=255)

                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                    >  5:303490/1‑137 (MQ=255)

                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                     <  1:239295/138‑2 (MQ=255)

                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                     <  3:22329/138‑2 (MQ=255)

                                                                                 cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                <  7:160376/138‑1 (MQ=255)

                                                                                 cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAGTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                >  5:212378/1‑138 (MQ=255)

                                                                                     aCCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaat                                            <  1:160159/138‑2 (MQ=255)

                                                                                      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAACATTCGTAAAAATTAATTGTGaaata                                           <  1:354834/138‑3 (MQ=255)

                                                                                      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                           <  6:406559/138‑3 (MQ=255)

                                                                                          tCACAATCCCGCTTATTCAGATTAAGAATACTCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      <  6:286548/139‑1 (MQ=255)

                                                                                          tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      <  8:276100/139‑1 (MQ=255)

                                                                                           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      <  6:210683/138‑1 (MQ=255)

                                                                                           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      <  6:334361/138‑1 (MQ=255)

                                                                                            acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                    <  2:280049/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGACTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  6:363742/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  6:414467/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  5:186329/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  6:303490/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  3:319231/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  7:184262/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  2:42555/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  4:378683/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  4:378499/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  4:298664/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  3:206882/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  7:466410/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  8:302149/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  8:35731/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  3:283642/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  3:342343/139‑1 (MQ=255)

                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGGGAAATACTTCACAata                                 <  6:15969/139‑1 (MQ=255)

                                                                                                tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                >  7:465397/1‑139 (MQ=255)

                                                                                                   cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                             >  6:94236/1‑139 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  7:44730/134‑1 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 >  8:44730/1‑134 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            <  4:268852/139‑1 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            <  6:297290/139‑1 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            <  5:94236/139‑1 (MQ=255)

                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            <  5:239672/139‑1 (MQ=255)

                                                                                                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 >  7:152029/1‑133 (MQ=255)

                                                                                                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 >  5:97195/1‑133 (MQ=255)

                                                                                                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                 <  8:152029/133‑1 (MQ=255)

                                                                                                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            <  5:89139/138‑1 (MQ=255)

                                                                                                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                            >  6:89139/1‑138 (MQ=255)

                                                                                                         ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       <  2:132587/139‑1 (MQ=255)

                                                                                                         ttCAGATTAAGAATACGCTTTCATCATAAATCAAGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTATATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       <  2:349678/139‑1 (MQ=255)

                                                                                                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTATCCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       >  1:241324/1‑138 (MQ=255)

                                                                                                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       <  3:175039/138‑1 (MQ=255)

                                                                                                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       <  6:379638/138‑1 (MQ=255)

                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCg                              <  6:41796/127‑1 (MQ=255)

                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCg                              >  5:41796/1‑127 (MQ=255)

                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                       <  8:423916/134‑1 (MQ=255)

                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                  >  5:147870/1‑139 (MQ=255)

                                                                                                               ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TATTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTTAAATAATTCACAATATAGTGCTATAatatt                  >  6:156286/4‑137 (MQ=255)

                                                                                                                  agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTc               <  6:238160/138‑1 (MQ=255)

                                                                                                                   gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaa             >  4:342401/1‑139 (MQ=255)

                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                <  4:179318/99‑1 (MQ=255)

                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      <  2:318352/109‑1 (MQ=255)

                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                      >  1:318352/1‑109 (MQ=255)

                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGATCAATCAt         >  4:258222/1‑138 (MQ=255)

                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGAAATAACATTAAGACAAAAATTCGTAAAAATTAATTGTGAAAAACTTCACAATATCCTTCTATACTATGCTAAATCAt         >  4:128279/1‑138 (MQ=255)

                                                                                                                             tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                           >  4:88107/1‑97 (MQ=255)

                                                                                                                             tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                           <  3:88107/99‑3 (MQ=255)

                                                                                                                              catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                           <  1:47493/98‑3 (MQ=255)

                                                                                                                              catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga   >  7:88632/1‑138 (MQ=255)

                                                                                                                              catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga   >  1:324490/1‑138 (MQ=255)

                                                                                                                               atcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa  <  4:209075/138‑1 (MQ=255)

                                                                                                                                   tAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                          <  7:316828/110‑1 (MQ=255)

                                                                                                                                   tAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                          >  8:316828/1‑110 (MQ=255)

AACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAA  >  CP006881/3722909‑3723172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`