Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 1,669,467 C→T R204* (CGA→TGA)  U712_08600 → Flagellar biosynthetic protein fliR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,669,4670CT98.8% 295.6 / ‑4.1 85R204* (CGA→TGA) U712_08600Flagellar biosynthetic protein fliR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (33/51);  total (33/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CGAATTTCGGCGATGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTCGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGATTGAAA  >  CP006881/1669333‑1669599
                                                                                                                                      |                                                                                                                                    
cGAATTTCGGCGATGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGaa                                                                                                                                   <  7:459759/138‑1 (MQ=255)
        ggCGATGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCg                                                                                                                           <  1:156341/138‑1 (MQ=255)
        ggCGATGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCg                                                                                                                           <  1:211828/138‑1 (MQ=255)
            aTGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGtt                                                                                                                      <  7:210710/139‑1 (MQ=255)
                aaaTTTTGCATATTTTATTGCTAAAAGGTTTAATGCAATGTTTATTATCGCTTTTCACATGTCAGCGCCGGTTGTGGCCATTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAAt                                                                                                                  <  8:373387/139‑1 (MQ=255)
                                aTTGCGAAAAGCTTTAATGCAATGTTTCTTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTggtcggt                                                                                                   <  8:171209/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCTAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTggtcggtc                                                                                                  <  5:108934/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTggtcggtc                                                                                                  <  1:471530/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTggtcggtc                                                                                                  <  3:187256/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTggtcggtc                                                                                                  <  5:96051/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTCGAACTGTTCCGCAGTTAAATGTATTTGTggtcggtc                                                                                                  <  1:336207/138‑1 (MQ=255)
                                 ttGCGAAAAGCTTTAATCCAATGTTTATTATCGATTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTATTTGATTTAGAATTAGGTATCGTGGCTTGAACTGTACCGCAGTTAATAGTATTTGTGGTCGGTCt                                                                                                 >  7:113391/1‑139 (MQ=255)
                                      aaaaGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCtt                                                                                            >  3:248570/1‑139 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGaa                                                                                                                                   <  6:421847/85‑1 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGaa                                                                                                                                   >  5:421847/1‑85 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGt                                                                               >  8:314943/1‑137 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGt                                                                               >  3:30019/1‑137 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGt                                                                               >  5:462409/1‑137 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGt                                                                               >  5:452000/1‑137 (MQ=255)
                                                     aTGTTTATTATCGCTTTTCAAATGTCAGCGCAGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAAATGTTCCGCAGTTAAATGTATTTGTGGTCTGTCTGCCTTTAAAGATCGCTGTCa                                                                             >  6:347617/1‑139 (MQ=255)
                                                      tGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAg                                                                            <  8:154263/139‑1 (MQ=255)
                                                      tGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAg                                                                            <  5:352775/139‑1 (MQ=255)
                                                      tGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAg                                                                            <  6:84640/139‑1 (MQ=255)
                                                           attatCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGtt                                                                                                                      >  6:383762/1‑92 (MQ=255)
                                                           attatCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGtt                                                                                                                      <  5:383762/92‑1 (MQ=255)
                                                            ttatCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCAt                                                                      <  8:84817/139‑1 (MQ=255)
                                                            ttatCGCTTTTCAAATGTCAGCGCCGCTGGTGGCCAGTATGTTTCTAGTTGAATTAGCATTAGGTATCGTGGCCTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCAt                                                                      <  2:472150/139‑1 (MQ=255)
                                                                 gCTTTTCAAATTTCAGAGACGGTTGTGGACAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATg                                                                  <  8:293070/138‑1 (MQ=255)
                                                                 gCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTTTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATg                                                                  <  8:3274/138‑1 (MQ=255)
                                                                 gCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATg                                                                  <  3:145342/138‑1 (MQ=255)
                                                                 gCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATg                                                                  <  6:452000/138‑1 (MQ=255)
                                                                  cTTTTGAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  6:349061/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTaaa                                                                                                                   >  1:201027/1‑88 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTaaa                                                                                                                   <  2:201027/88‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCTGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  7:283924/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                >  3:374623/1‑139 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  7:168111/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                >  7:11660/1‑139 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  6:462409/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                >  3:200246/1‑139 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  7:314943/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  4:402313/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                <  4:406316/139‑1 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                >  5:183235/1‑139 (MQ=255)
                                                                  cTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCt                                                                >  5:251133/1‑139 (MQ=255)
                                                                    tttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              >  2:18015/1‑139 (MQ=255)
                                                                    tttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              >  6:412104/1‑139 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  3:275784/138‑1 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  8:62774/138‑1 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  4:200246/138‑1 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  8:42289/138‑1 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  7:94247/138‑1 (MQ=255)
                                                                     ttCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  8:11660/138‑1 (MQ=255)
                                                                       cAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCAtt                                                            <  4:189507/138‑1 (MQ=255)
                                                                        aaaTGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATtgt                                                          <  4:374623/139‑1 (MQ=255)
                                                                               aGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTgtatgtat                                                    <  5:358706/138‑1 (MQ=255)
                                                                                      gTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATtgtatgta                                                     <  7:219748/130‑1 (MQ=255)
                                                                                      gTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATtgtatgta                                                     >  8:219748/1‑130 (MQ=255)
                                                                                            gCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTg                                                                                                <  3:338979/81‑1 (MQ=255)
                                                                                            gCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTg                                                                                                >  4:338979/1‑81 (MQ=255)
                                                                                               aGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTtcgtcg                                   <  6:419947/139‑1 (MQ=255)
                                                                                                 tttgtttCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGCGGTCGGTCTGCCTTTAAAGATCGCTGTCAg                                                                            <  5:336983/96‑1 (MQ=255)
                                                                                                 tttgtttCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGAGGTCGGTCTGCCTTTAAAGATCGCTGTCAg                                                                            >  6:336983/1‑96 (MQ=255)
                                                                                                     tttCTAGTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTcc                             <  3:332652/139‑1 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCAtt                                                                     <  3:21921/93‑1 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCAtt                                                                     >  4:21921/1‑93 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGa                          <  1:18015/136‑1 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGa                          <  5:412104/136‑1 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATg                       >  3:141140/1‑139 (MQ=255)
                                                                                                           gTTGATTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGACCGTTATCTTCGTCGTTGTCCGGAATg                       >  6:102682/1‑139 (MQ=255)
                                                                                                               aTTTAGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGtttt                   <  3:471256/139‑1 (MQ=255)
                                                                                                                   aGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGtt                                                                          <  6:314113/80‑1 (MQ=255)
                                                                                                                   aGCATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGtt                                                                          >  5:314113/1‑80 (MQ=255)
                                                                                                                     cATTAGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGtt              >  1:247931/1‑138 (MQ=255)
                                                                                                                         aGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              >  3:439745/1‑86 (MQ=255)
                                                                                                                         aGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCa                                                              <  4:439745/86‑1 (MQ=255)
                                                                                                                         aGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAAc          >  2:152673/1‑138 (MQ=255)
                                                                                                                         aGGTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAAc          >  3:419400/1‑138 (MQ=255)
                                                                                                                          ggTATCGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGa        >  8:271800/1‑139 (MQ=255)
                                                                                                                               cGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCAtt                                                            >  3:379354/1‑82 (MQ=255)
                                                                                                                               cGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCAtt                                                            <  4:379354/82‑1 (MQ=255)
                                                                                                                               cGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCTTTGTCCGGAATGTTTTCAGTTTAACGATTGa    >  5:62772/1‑138 (MQ=255)
                                                                                                                               cGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGATTGa    >  3:314211/1‑138 (MQ=255)
                                                                                                                               cGTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGATTGa    >  7:385299/1‑138 (MQ=255)
                                                                                                                                gTGGCTTGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGATTGaaa  <  4:141140/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CGAATTTCGGCGATGAAAAGTTTGCCTATTTTATTGCGAAAAGCTTTAATGCAATGTTTATTATCGCTTTTCAAATGTCAGCGCCGGTTGTGGCCAGTTTGTTTCTAGTTGATTTAGCATTAGGTATCGTGGCTCGAACTGTTCCGCAGTTAAATGTATTTGTGGTCGGTCTGCCTTTAAAGATCGCTGTCAGTTTCATTATGCTCATTGTATGTATGTCCGTTATCTTCGTCGTTGTCCGGAATGTTTTCAGTTTAACGATTGAAA  >  CP006881/1669333‑1669599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑